Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules, and for the isolation of cloned genetic sequences that determine these structures

ABSTRACT

A method for isolating a gene, comprising: 
     (i) isolating a cell possessing a post-translational characteristic of interest, said post-translational characteristic being the presence of a membrane-bound oligosaccharide or polysaccharide of interest on the surface of said cell, the presence of a soluble oligosaccharide or polysaccharide of interest in an extract of said cell, or the presence of a particularly glycosyltransferase activity in an extract of said cell; 
     (ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of said isolated cell; 
     (iii) transforming host cells with said genetic library; and 
     (iv) screening said transformed host cells for a host cell containing said post-translational characteristic, thereby obtaining a cell containing said gene, is disclosed. The method can be used to obtain genes encoding glycosyltransferases.

This is a Division of application Ser. No. 08/393,246 filed on Feb. 23, 1995, now U.S. Pat. No. 5,595,900, which is a Continuation of application Ser. No. 08/220,433, filed Mar. 30, 1994, abandoned, which is a Division of application Ser. No. 07/914,281, filed Jul. 20, 1992, now U.S. Pat. No. 5,324,663, which is a Continuation-in-Part of application Ser. No. 07/715,900, filed Jun. 19, 1991, abandoned, which is a Continuation-in-Part of application Ser. No. 07/627,621, filed Dec. 12, 1990, abandoned, which is a Continuation-in-Part of application Ser. No. 07/479,858, filed Feb. 14, 1990, abandoned.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to methods and products for the synthesis of oligosaccharide or polysaccharide structures, on glycoproteins, glycolipids, or as free molecules.

2. Discussion of the Background

Carbohydrates are an important class of biological compounds which are remarkable for their structural diversity. This diversity is not random but rather consists of specific sets of oligosaccharide structures that exhibit precise tissue-specific and developmental expression patterns. In cells carbohydrates function as structural components where they regulate viscosity, store energy, or are key components of cell surfaces. Numerous site specific intercellular interactions involve cell surface carbohydrates. For example, union of sperm and egg as well as the implantation of fertilized egg are both mediated by cell surface carbohydrates. Likewise, a number of proteins that function as cell adhesion molecules, including GMP-140, Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1), and lymphocyte adhesion molecules like Mel-14, exhibit structural features that mimic lectins, and are now known to bind specific cell surface carbohydrate structures (Feizi, Trends Biochem. Sci. (1991) 16:84-86). Glycosylated proteins as tumor-associated antigens are now being used to identify the presence of numerous carcinomas. Even isolated oligosaccharides have been found to exhibit biological activity on their own.

Specific galactose oligosaccharides are known to inhibit the agglutination of uropathogenic caliform bacteria with red blood cells (U.S. Pat. No. 4,521,592). Other oligosaccharides have been shown to possess potent antithrombic activity by increasing the levels of plasminogen activator (U.S. Pat. No. 4,801,583). This same biological activity has been used, by binding oligosaccharides, in conjunction with an amino glycoprotein, in medical instruments to provide medical surfaces which have anticoagulation effects (U.S. Pat. No. 4,810,784). Still other oligosaccharides have found utility as gram positive antibiotics and disinfectants (U.S. Pat. Nos. 4,851,338 and 4,665,060). Further, oligosaccharides have been used as bacteria receptor sites in the diagnosis and identification of specific bacteria (U.S. Pat. Nos. 4,657,849 and 4,762,824).

It is also well recognized that oligosaccharides have an influence on the protein or lipid to which they are conjugated (Rademacher et al, Ann. Rev. Biochem., (1988) 57:785). Specific oligosaccharides have been shown to influence proteins' stability, rate of in vivo clearance from blood stream, rate of proteolysis, thermal stability and solubility. Changes in the oligosaccharide portion of cell surface carbohydrates have been noted in cells which have become cancerous. Other oligosaccharide changes have been detected during cell differentiation (Toone et al, Tetrahedron Report (1989) 45(17):5365-5422). As such, the significance of oligosaccharides to biological function cannot be understated.

The fundamental role of these materials in molecular biology has made them the object of considerable research, in particulars considerable efforts have been made in organic synthesis to synthesize these materials. Although synthetic approaches to making carbohydrates are quite developed, this technique suffers notable difficulties which relate to the selective protection and deprotection steps required in the available synthetic pathways. These difficulties, combined with difficulties associated with isolating and purifying carbohydrates, and determining their structures, has made it essentially impossible for synthetic organic chemistry to economically produce valuable carbohydrates.

Enzyme-mediated catalytic synthesis would offer dramatic advantages over the classical synthetic organic pathways, producing very high yields of carbohydrates (e.g., oligosaccharides and/or polysaccharides) economically, under mild conditions in aqueous solution, and without generating notable amounts of undesired side products. Such enzymes, which include glycosyltransferases, are however difficult to isolate, especially from eukaryotic, e.g., mammalian sources, because these proteins are only found in low concentrations, and are membrane-bound.

As of 1987, standard molecular cloning approaches which require amino acid sequence information or anti-glycosyltransferase antibodies, had been successfully used to isolate just two eukaryotic, e.g., mammalian glycosyltransferase cDNAs, corresponding to β(1,4)galactosyltransferase (in 1986) and α(2,6)sialyltransferase (in 1987). In light of the above-noted considerable value of carbohydrates, there is accordingly a strongly felt need for an improved method for isolation of additional glycosyltransferase genes and cDNAs and for their use in carbohydrate synthesis.

SUMMARY OF THE INVENTION

Accordingly, it is an object of this invention to provide a method for readily isolating eukaryotic, e.g., mammalian glycosyltransferase genes and cDNAs.

It is another object of this invention to provide a method to modify these isolated genes and cDNAs to obtain correspondingly modified glycosyltransferases.

It is another object of this invention to provide these unmodified and modified isolated genes and cDNAs, and to use them, for example, in modifying cell surface oligosaccharide structure via gene transfer approaches or via in vitro glycosylation reactions.

The inventor has now discovered a gene transfer approach which satisfies all of the above-noted objects of this invention, and other objects which will be seen from the description of the invention given hereinbelow. The present methodology takes advantage of existing information about substrate and acceptor properties of glycosyltransferases and makes use of the numerous antibody and lectin reagents that are specific to the cell surface-expressed oligosaccharide products of these enzymes.

BRIEF DESCRIPTION OF THE FIGURES

FIGS. 1, 2, 3, 4, 5, 6, and 7 provide six DNA sequences provided by the invention, encloding glycosyltransferases.

FIG. 1 shows the DNA sequence encoding a protein capable of functioning as a GDP-Fuc: β-D-Gal(1,4/1,3)-D-GlcNac(/Glc)-α-(1,3/1,4)-fucosyltransferase (Lewis fucosyltransferase, Fuc-TIII) (SEQ ID NO:1) and the amino acid sequence of the encoded protein (Fuc-TIII)(SEQ ID NO:2).

FIG. 2 shows the DNA sequence encoding a mouse UDP-Gal:β-D-Gal-(1,4)-D-GlcNac α(1,3)-galactosyltransferase (SEQ ID NO:3) and the encoded protein (SEQ ID NO:4).

FIG. 3 shows the DNA sequence encoding a human GDP-Fuc:β-D-galactoside α(1,2)-fucosyltransferase (SEQ ID NO:5) and the amino acid sequence of the encoded protein (SEQ ID NO:6).

FIGS. 4 and 5 provide DNA sequences (SEQ ID NO:7 and SEQ ID NO:9, respectively) encoding a GDP-Fuc: β-D-Gal(1,4)!-D-GlcNacα(1,3)-fucosyltransferase (Fuc-TIV) and the encoded protein (Fuc-TIV) (SEQ ID NO:8). FIG. 5 also shows the amino acid sequence of the Lewis fucosyltransferase (Fuc-TIII) (SEQ ID NO:2).

FIG. 6 provides a DNA sequence (SEQ ID NO:10) encoding a GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAcα(1,3)-fucosyltransferase (Fuc-TV) (numbered upper nucleotide strand) and its corresponding protein sequence (Fuc TV)(SEQ ID NO:11), together with the DNA sequence of the Lewis blood group fucosyltransferase (Fuc-TIII) (unnumbered lower sequence). Amino acid differences with the Lewis fucosyltransferase are indicated by inclusion of Lewis amino acids below the Lewis DNA sequence (SEQ ID NO:12). The transmembrane domain of the fucosyltransferase is underlined.

FIG. 7 provides a DNA sequence of the coding portion of the genomic DNA insert in pCDNAl-α(1,3)Fuc-TVI (SEQ ID NO:13), and parts of the 5' and 3' regions of that gene. DNA sequence comparison between the GDP-Fuc: β-D-Gal(1,4)!-D-GlcNacα(1,3)-fucosyltransferase (Fuc-TVI) encoded by the genomic DNA fragment in pCDNAl-α(1,3)Fuc-TVI (labeled α(1,3)FT DNA) and the Lewis blood group fucosyltransferase (Fuc-TIII) (labeled Lewis DNA) (SEQ ID NO:12) is also shown in FIG. 7. Positions of DNA sequence identity are denoted by a vertical line (|) between identical nucleotides at similar positions. Positions where the sequences are out of register are denoted by (.). The derived protein sequence of Fuc-TVI, in single letter code, and labeled α(1,3)FT AA, is indicated above its DNA sequence (SEQ ID NO:14).

FIG. 8 presents flow cytometry profile histograms of COS-1 cells transfected with different α(1,3)fucosyltransferase gene expression vectors, or with control vectors, and subjected to analysis with monoclonal antibodies directed against cell surface oligosaccharide determinants. COS-1 cells were transfected either with plasmid pCDM7 (pCDM7), with plasmid pCDNAI(pCDNAI), with pCDM7 containing DNA sequences encoding the α(1,3/1,4)fucosyltransferase depicted in FIG. 1 (the Lewis fucosyltransferase, also known as Fuc-TIII, SEQ ID NO:2), or with pCDNAI containing DNA sequences encoding the α(1,3)fucosyltransferase depicted in FIG. 4 (Fuc-TIV, SEQ ID NO:8), the α(1,3)fucosyltransferase depicted in FIG. 6 (Fuc-TV, SEQ ID NO: 11), or the α(1,3)fucosyltransferase depicted in FIG. 7 (Fuc-TV, SEQ ID NO:14). Three days after transfection, the cells were harvested, stained with monoclonal antibodies (shown at the top left within the figure) directed against the H (anti-H), Lewis x (anti-Lex), sialyl Lewis x (anti-sLex), Lewis a (anti-Lea), or sialyl Lewis a (sLea) oligosaccharide determinants, and then stained with a fluorescein-conjugated second antibody. The cells were then subjected to analysis by flow cytometry. The histograms represent the mean fluorescent intensities of the antigen-positive cells in each transfectant population (approximately 25% to 30% of the cells are transfected and express the positive cell surface markers). Methods for these analyses have been described in detail in Lowe et al, J. Biol. Chem., (1991), 266:17467-17477, Weston et al, J. Biol. Chem., (1992), 267:4152-4160, Lowe et al, Cell, (1990), 63:475-484, and Ernst et al, J. Biol. Chem., (1989), 264:3436-3447.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

Generally, the present invention provides a method for isolating a gene and/or a cDNA from a cell, by using a post-translational characteristic of the cell. The cell from which this gene and/or cDNA may be isolated may be either a cell from a unicellular or a multicellular organism.

In the context of the present invention, a post-translational characteristic of a certain cell is defined by the ability of that cell to modify a protein or a lipid by an enzymatic process that covalently attaches to this protein or lipid one or more monosaccharides, or an enzymatic process that specifically removes such substituents from a protein or lipid molecule.

In one embodiment, the method comprises the following four basic steps:

(i) identifying for use as a genetic donor, a eukaryotic (e.g. mammalian) cell possessing a post-translational characteristic of interest i.e. a particular membrane-bound oligosaccharide or polysaccharide (i.e. a glycoprotein or glycolipid), soluble oligosaccharide or polysaccharide, or a particular enzymatic activity (vide infra);

(ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of the donor eukaryotic (e.g. mammalian) cell;

(iii) identifying a specific eukaryotic host suitable as a recipient for gene transfer, and transforming this eukaryotic, (e.g. mammalian), host cells with this genetic library; and

(iv) screening the transformed host cells for host cells possessing the post-translational characteristic of interest.

The host cell which now possesses this post-translational characteristic contains genetic information related to the post-translational characteristic of interest. Using the techniques set forth below this genetic information (gene) can then be retrieved from the transformed host cell and used by standard approaches i.e., Axel et al (U.S. Pat. No. 4,634,665) or Gilbert et al (U.S. Pat. No. 4,411,994) to produce large quantities of the gene product, i.e., the glycosyltransferase, responsible for the post-translational characteristic.

In step (i) above the donor eukaryotic (e.g. mammalian) cell is chosen on the basis of detecting a specific enzymatic activity in an extract of the cell or detecting a membrane-bound or soluble oligosaccharide or polysaccharide of the cell.

Thus in one embodiment the enzymatic activity which is detected in the cell extract can be enzymatic activity attributable to an animal enzyme which post-translationally modifies proteins, lipids, or oligosaccharides by glycosylation or glycosyl modification. This enzymatic activity may be detected by using a substrate specific for one of these enzymes. Such substrates are known.

In another embodiment, in step (i) above a cell is chosen on the basis of detection of a specific cellular membrane-bound oligosaccharide and/or polysaccharide.

In another embodiment, the cell in step (i) is chosen on the basis of detecting the presence of a soluble oligosacharride or polysaccharide in an extract of the cell, or released by the cell in soluble form.

The present invention provides a novel gene transfer approach designated to isolate genes from an organism without requiring that amino acid sequence information be obtained about the gene product or that an antibody specific to the gene product be available. For example, if a gene encoding a particular enzyme is sought, a series of cultured cell lines or tissues are screened by known and standard methods to identify one or more cell lines or tissues containing an expressible gene of interest by detecting in an extract of the cell or tissue specific enzymatic activity (corresponding to the enzyme of interest and thus the cell contains and/or expresses a gene for the enzyme sought). If an oligosaccharide or polysaccharide membrane component of the cell is of interest, a cell line or tissue possessing such a membrane characteristic is isolated. If a soluble oligosaccharide or polysaccharide is of interest, a cell line or tissue possessing a soluble oligosaccharide or polysaccharide detectable in an extract thereof is isolated.

Once such a cell line or tissue has been identified, a genetic library based on this isolated cell is created. This genetic library may be either cDNA or genomic DNA. In a preferred embodiment, if the isolated cell is known to be susceptible to enhancement of its post-translational characteristic of interest by being contacted with a particular reagent, this reagent is used to obtain an enhancement of the mRNA signal in this cell, and/or the gene itself, which consequently produces amplified in mRNA copies and thus ultimately cDNA copies, corresponding to that particular gene, or amplified gene segments. Both the cDNA and the genomic DNA genetic libraries may otherwise be obtained using known techniques. Once the genetic library is obtained, it is used to transform host cells using known techniques (e.g., by calcium phosphate precipitation, liposomal transfection, DEAE dextran transfection, microinjection, etc.).

Host cells useful in the present invention are preferably susceptible to lectin or antibody detection of the desired post-translational characteristic; that is, susceptible to lectin or antibody detection of membrane-bound oligosaccharide, polysaccharide, or of glycoprotein or glycolipid produced in the transformed host cell. However, screening of host cells not susceptible to such lectin or antibody detection may be achieved through screening for enzyme activity in accordance with the invention.

(A) Host (e.g. mammalian) cells should be eukaryotic cells to allow and preserve the catalytic function of the enzyme (the glycosyltransferase). (B) The host cell should not express significant levels of glycosyltransferase activity analogous to the desired one, or the cognate product. With glycosyltransferase-related genes, successful transformation of a host cell can be determined by detecting corresponding enzymatic activity in an extract of the cell. (C) In another characteristic, the host cell should be capable of synthesizing the appropriate sugar nucleotide substrate and transport it into Golgi (where glycosyltransferase catalytic domains exist and function). Virtually all wild type animal cells possess this function. (D) The host cell should possess the ability to synthesize the appropriate acceptor substrate (oligosaccharide, lipid, or protein) that the desired glycosyltransferase requires, and the cell must display the structure on the cell surface or release it into the cellular environment/media. (E) The host cell should allow or provide for expression of transfected sequences that encode or otherwise determine expression of the desired glycosyltransferase. This is inherent in eukaryotic (e.g. mammal) to eukaryotic (e.g. mammal) genomic DNA transfer, or in vector systems chosen for cDNA expression system, using known technology. (F) The host cell should allow for rescue of the transfected sequences that encode or otherwise determine expression of the relevant glycosyltransferase.

Wild-type eukaryotic (e.g. mammalian) cells possess these characteristics generally. Any particular wild-type cell of interest which does not possess criteria (B) or (D) set forth above, may be mutated, using standard techniques, to obtain a mutant cell possessing either of these criteria. If an enzyme assay-based selection method is used, then the criteria (C) and (D) set forth above are not necessary.

Once the host cells have been transformed, the population is screened for host cells containing the genetic material of interest. This is achieved by determining whether the host cell possesses the post-translational characteristic of interest, i.e., by detecting enzymatic activity in an extract of the transformed host cell, detecting membrane-bound oligosaccharide or polysaccharide on the cell, or detecting soluble oligosaccharide or polysaccharide in an extract of or secreted by the cell. The host cells which test positive are isolated, and the gene of interest can be retrieved from these transformed cells.

If the host cells are transformed by genomic DNA transfection, the gene rescue may be carried out as follows:

(a) molecular cloning by hybridization, via tagging of transfected genomic sequences by species-specific repetitive sequences; or

(b) tagging of transfected genomic sequences by in vitro or in vivo ligation to marker sequences.

If cDNA is used to transform the host cells, the gene/cDNA rescue may be carried out as follows:

(a) episomal rescue via Hirt procedure, or

(b) integrated copy rescue via plasmid tag.

Further detail with regard to gene rescue is provided in the accompanying examples.

Example of appropriate donor and host cells include the following:

(I) Human Blood Group H α(1,2)fucosyl-transferase--(Ernst et al, J. Biol. Chem. 264:3436-3447, 1989; Ralan et al, J. Biol. Chem. 264:11158-11167, 1989; Larsen et al, Proc. Natl. Acad. Sci. USA, 87:6674-6678, 1990).

A.) L cell host-mouse species.

B.) Does not express α(1,2)fucosyltransferase activity. Does not express Fucα(1,2)Gal structures on cell surface.

C.) Does synthesize GDP-fucose, the sugar nucleotide substrate of α(1,2)fucosyltransferase.

D.) Does synthesize Galβ(1,4)GlcNAc-R molecules that are acceptor substrates for the enzyme, and expresses them at cell surface.

E.) Mouse cells are known to be able to express human genes.

F.) Mouse cells do not contain DNA sequences similar to human Alu repetitive DNA sequences. These Alu sequences (species-specific repetitive sequences) were used to identify and ultimately rescue the human gene from the mouse transfectant cell line.

(II) Mouse α(1,3)galactosyltransferase--Larsen et al, Proc. Natl. Acad. Sci. USA, 86: 8227-8231, 1989.

A.) Kidney cell line expressing SV40 virus large T antigen--COS-1 cell line, monkey species.

B.) Does not express α(1,3)galactosyltransferase activity. Does not express Galα(1,3)Gal structures on cell surface.

C.) Does synthesize UDP-galactose, substrate of α(1,3)galactosyltransferase.

D.) Does synthesize Galβ(1,4)GlcNAc-R molecules that are acceptor substrates for the enzyme, and expresses them at cell surface.

E.) cDNA/COS-1 cell expression system for cDNA libraries--standard technology.

F.) cDNA/COS-1 cell expression system for cDNA libraries--standard technology.

(III) Human Lewis Blood Group α(1,3/1,4)fucosyl-transferase--J. F. Kukowska-Latallo et al, Genes and Development, vol. 4, (1990), pp. 1288-1303.

A.) Kidney cell line expressing SV40 large T antigen COS-1 cell line, monkey.

B.) Does not express significant levels of α(1,3)fucosyltransferase activity. Does not express cell surface Galβ(1,4) Fucα(1,3)!GlcNAc-R structure.

C.) Does synthesize GDP-fucose, substrate of α(1,3)fucosyltransferase.

D.) Does synthesize Galβ(1,4)GlcNAc-R molecules that are acceptor substrates for the enzyme, and expresses them at cell surface.

E.) CDM 7/COS-1 cell expression system for cDNA libraries--standard technology.

F.) CDM 7/COS-1 cell expression system for cDNA libraries--standard technology.

In the latter stages of selection for this gene, criteria C and D were not necessary because pools of cDNA clones were screened by transfecting them into COS-1 cells, and then directly assaying extracts prepared from the transfected cells for α(1,3)fucosyltransferase activity.

In one of its embodiments, the present invention provides a method for isolating a gene encoding a glycosyltransferase, and the gene thus isolated. This glycosyltransferase may be a fucosyltransferase, a sialyltransferase, a N-acetylglucosaminyltransferase, a galactosyltransferase, a N-acetylgalactosaminyltransferase, a mannosyltransferase, a sulfotransferase, a glucosyltransferase, an acetylase, or another glycosyltransferase.

Individual glycosyltransferases are known to be particularly related to different types of sugars transferred by the enzyme (Beyer et al, "Glycosyltransferases and Their Use in Assessing Oligosaccharide Structure and Structure-Function Relationships" Adv. Enzymology (1982) 52: 23-175--hereby incorporated by reference). Thus a particular kind of sugar linkage found on an oligosaccharide, glycoprotein, or glycolipid in or on a cell is associated with a particular glycosyltransferase. Methods are known for identifying such linkages (see Beyer et al, supra), and can be used in accordance with the present invention to isolate the gene encoding the corresponding glycosyltransferase.

Sialyltransferases, one of the glycosyltransferase provided by the present invention, are associated with the following sialic acid linkages: (1) Siaα2→6Gal; (2) Siaα2→3Gal; (3) Siaα2→6GalNac; (4) Siaα2→6GlcNAc; (5) Siaα2→8Sia; (6) Siaα2→4Gal; and (7) Siaα2→4GlcNAc.

Fucosyltransferases, another type of glycosyltransferases provided by the present invention, are associated with the following linkages: (1) Fucα(1→2)Galβ-; (2) Galβ(1→3) Fucα(1→4)!GlcNAcβ-; (3) Galβ(1→4) Fucα(1→3)!GlcNAcβ-; (4) Galβ(1→4)(Fucα(1→3)!Glc; (5) -GlcNAcβ(1→4) Fucα(1→6)!GlcNAcβ1→Asn; (6) -GlcNAcβ(1→4) Fucα(1→3)GlcNAcβ1→Asn; (7) Fucα(1→6)Galβ-; (8) Fucα(1→3)Galβ-; (a) Glcβ1→3Fucα1→O-Thr and Fucα1→O-Thr/Ser; (10) Fucα1→Ceramide; and (11) Fucα1→3Fuc.

N-Acetylglucosaminyltransferases, also provided by the invention, are associated with the following linkages: (1) GlcNAcβ1→4GlcNAc; (2) GlcNAcβ1→Asn; (3) GlcNAcβ1→2Man; (4) GlcNAcβ1→4Man; (5) GlcNAcβ1→6Man; (6) GlcNAcβ1→3Man; (7) GlcNAcα1→3Man; (8) GlcNAcβ1→3Gal; (9) GlcNAcβ1→4Gal; (10) GlcNAcβ1→6Gal; (11) GlcNAcα1→4Gal; (12) GlcNAcα1→4GlcNAc; (13) GlcNAcβ1→6GalNAc; (14) GlcNAcβ1→3GalNAc; (15) GlcNAcβ1→4GlcUA; (16) GlcNAcα1→4GlcUA; (17) GlcNAcα1→4IdUA.

Galactosyltransferases, also provided by the invention, are associated with the following linkages: (1) Galβ1→4Glc; (2) Galβ1→4GlcNAc; (3) Galβ1→3GlcNAc; (4) Galβ1→6GlcNAc; (5) Galβ1→3GalNAc; (6) Galβ1→6GalNAc; (7) Galα1→3GalNAc; (8) Galα1→3Gal; (9) Galα1→4Gal; (10) Galβ1→3Gal; (11) Galβ1→4Gal; (12) Galβ1→6Gal; (13) Galβ1→4xylose; (14) Galβ1→1'-sphingosine; (15) Galβ1→1'-ceramide; (16) Galβ1→3 diglyceride; (17) Galβ1→-O-hydroxylysine; and (18) Gal-S-cysteine.

N-Acetylgalactosaminyltransferases also provided by the invention are associated with the following linkages: (1) (GalNAcα1→3) (Fucα1→2)!Galβ-; (2) GalNAcα1→Ser/Thr; (3) GalNAcβ1→4Gal; (4) GalNAcβ1→3Gal; (5) GalNAcα1→3GalNAc; (6) (GalNAcβ1→4GlcUAβ1→3)_(n) ; (7) (GalNAcβ1→41dUAα1→3-)_(n) ; (8) -Manβ→GalNAc→GlcNAc→Asn.

Other glycosyltransferases, also provided by the invention, are associated with the following linkages:

To GalNAc

Galβ1-3GalNAc

Galβ1-4GalNAc

Galα1-3GalNAc

GlcNAcβ1-3GalNAc

GlcNAcβ1-6GalNAc

GalNAcα1-3GalNAc

Siaα2-3GalNAc

Siaα2-6GalNAc

To Gal

Galβ1-3Gal

Galα1-3Gal

Fucα1-2Gal

GlcNAcβ1-3Gal

GlcNAcβ1-4Gal

GlcNAcβ1-6Gal

GlcNAcα1-4Gal

GalNAcα1-3Gal

GalNAcβ1-3Gal

GalNAcβ1-4Gal

Siaα2-3Gal

Siaα2-6Gal

To Glc

Manα1-6Glc

Manα1-4Glc

To GlcNAc

Galβ1-4GlcNAc

Galβ1-3GlcNAc

Fucα1-3GlcNAc

Fucα1-4GlcNAc

Glcα1-4GlcNAc

GlcNAcα1-4GlcNAc

Siaα2-4GlcNAc

To Sia

Siaα2-8Sia

To Protein

GalNAcα1-O-Ser/Thr

Still other glycosyltransferases provided by the invention include: β1,3GlcNAc-β1,3glucuronyltransferase, glucuronic acid-β1,4-N-acetylglucosaminyltransferase, asparagine N-acetylglucasaminyltransferase, serine β-xylosyltransferase, xylose β1,4-galactosyltransferase, galactose β1,3-galactosyltransferase, galactose β1,3-glucuronyltransferase, glucuronic acid β1,4-N-acetylgalactosaminyltransferase, N-acetylgalactosamine β1,3-glucuronyltransferase, N-acetylgalactosamine-4-sulfotransferase, N-acetylgalactosamine-6-sulfotransferase, asparagine-βN-acetylglucosaminyltransferase, serine/threonine-αN-acetylgalactosaminyltransferase, N-acetylglucosamine-β1,4-galactosaminyltransferase, galactose-β1,3-N-acetylglucosaminyltransferase, N-acetylglucosamine-6-sulfotransferase, galactose-6-sulfotransferase, glucuronic acid-α1,4-N-acetylglucosaminyltransferase, N-acetylglucosamine β1,4-glucuronyltransferase, heparin-N-acetyl-glucosamine-N-acetyltransferase, galactose-1,6-N-acetylgalactosyltransferase, heparin-N-acetylglucosamine sulfotransferase, N-acetylglucosamine-α1,4-glucoronyl epimerase, N-acetylglucosamine-6-sulfotransferase, N-acetylglucosamine-N-sulfotransferase, glucuronyl-α1,4-N-acetylglucosaminyltransferase, Iduronyl-2-sulfotransferase, glucuronyl-β1,4-N-acetylgalactosaminyltransferase, and N-acetylgalactosamine-α1,3-glucuronyl epimerase.

These enzymes are associated with the following linkages and oligosaccharide structures in connective tissue polysaccharides (Roden, L. "Structure and Metabolism of Connective Tissue Proteoglycans," in The Biochemistry of Glyc2cograteoproteins and Proteoglycans, Wm. Lennarz, ed. pp. 267-371 Plenum Press, New York, incorporated herewith by reference, in particular the tables on pages 269, 270 and 271 thereof).

The above-noted glycosyltransferase genes and/or cDNAs are obtained as cloned molecules, or are transferred to host cell lines, in accordance with the invention by using the post-translational property manifest in the cognate and appropriate above-noted characteristic linkages, to isolate a cell from which the gene or cDNA library is then created, and from which the gene or cDNA encoding the glycosyltransferase is isolated.

Additional enzymes comprising members of the mannosyltransferase family include α(1,2) mannosyltransferases, α(1,3) mannosyltransferases, α(1,6) mannosyltransferases, and β(1,4) mannosyltransferases, associated with the construction of the linkages formed in asparagine-linked oligosaccharides, as exemplified below (and as reviewed in Kornfeld, F., and Kornfeld, S. (1985)) "Assembly of asparagine-linked oligosaccharides" Annu. Rev. Biochem. 54, pp. 631-664.) ##STR1##

Others include ceramide glucosyltransferase and ceramide galactosyltransferase, oligosaccharyltransferase, and O-acetylases that O-acetylate N-acetylneuraminic acid (sialic acid).

    ______________________________________     Abbreviations:     ______________________________________     Sia; sialic acid    IdUA: L-iduronic acid     Gal; D-galactose    GlcUA; D-glucuronic acid     GalNac; D-N-acetylgalactosamine                         Xyl; D-xylose     Glc; D-glucose      Ser; serine     GlcNAc; D-N-acetylglucosamine                         Thr; Threonine     Fuc; L-fucose       Asn; asparagine--.     Man; D-mannose     ______________________________________

In another embodiment, the present invention provides a method for obtaining a soluble or a solid-phase oligosaccharide, polysaccharide, lipid, or protein. This method comprises contacting an oligosaccharide or polysaccharide precursor with a fused protein. The enzyme used, provided by the present invention, is either an unglycosylated glycosyl transferase or a fused protein which comprises two moieties: as the first moiety, at least the catalytically functional domain of a glycosyltransferase (vide infra); and, as a second moiety, either a proteinaceous spacer attached to the solid support or a proteinaceous component comprising an affinity ligand. The enzyme of the invention transforms the precursor into the desired oligosaccharide, polysaccharide, glycolipid, or glycoprotein which is thereby obtained.

A notable advantage of the invention is that it may provide, in one embodiment, non-glycosylated glycosyltransferases. It is thought to be generally true that many (if not all) naturally occurring glycosyltransferases are glycoproteins. When they are used to produce oligosaccharides or polysaccharides from oligosaccharide/polysaccharide precursors, the enzymes themselves may be susceptible to glycosylation. This (undesired) activity may consume starting material and may result in premature loss of enzyme activity. The non-glycosylated glycosyltransferases of the present invention do not suffer these salient disadvantages. They may be obtained as non-glycosylated enzyme either because they are obtained as a product produced in a microorganism deficient in the relevant glycosylation mechanism, or in an animal cell in which glycosylation of the glycosyltransferase has been suppressed. Suppression of the glycosylation of the glycosyltransferase in an animal cell is obtained by mutating the isolated glycosyltransferase gene using known techniques to eliminate the glycosyation sites on the glycosyltransferase.

The non-glycosylated glycosyltransferase of the present invention can be a non-glycosylated protein corresponding at least to the catalytically functional domain of the glycosyltransferase (vide infra) and up to a non-glycosylated protein corresponding to the whole gene encoding the glycosyltransferase.

In another embodiment, the present invention provides a fused protein comprising two moieties as set forth above: as a first moiety, at least the catalytically functional domain of a glycosyltransferase; and, as a second moiety, either a proteinaceous spacer capable of being attached to a solid support or a proteinaceous component comprising an affinity ligand.

Glycosyltransferases are known to possess three domains which correspond to three different areas of the gene encoding the enzyme. The area of the gene found at the 3'-end of the gene is known to encode the catalytically functional domain (Lowe, Seminars in Cell Biology, (1991) 2:289-307 hereby incorporated by reference). The fused protein of the present invention contains at least this catalytically functional domain, but it can contain up to the whole protein sequence. The protein is produced fused to the second moiety using known techniques.

The second moiety can be used either to anchor the catalytically functional domain onto a solid support, or permit its recovery by exploiting the presence of a specific affinity ligand on the second moiety. For the second moiety, the IgG binding domain of Staph. protein A can be used. Such a fusion protein can be bound to an IgG-containing solid phase matrix, such as IgG-Sepharose. A number of other alternative proteins can be fused to the catalytically active segments of the glycosyltransferase to effect binding to a solid matrix. Such proteins, and their respective matrix-associated receptors include streptavidin-biotin, IgG heavy chain-protein A, and virtually any other known protein or peptide segment for which an antibody exists or can be made.

In another embodiment, the present invention provides a method for producing a recombinant glycoprotein, glycolipid or free oligosaccharide using, e.g., either an enzyme obtained in accordance with the present invention or a recombinant organism obtained in accordance with the present invention. For example, specific post-translational glycosylation capability can be added to a host cell by the following steps: first the desired gene or cDNA which has been isolated is introduced into a cell by using standard transformation or transfection techniques in a manner to obtain an organism capable of expression of the transfected cloned gene product; the host cell acquires the post-translational capability determined by the transfected gene, where the cell did not express this capability prior to transfection. Alternatively, the approach set forth above is performed, but instead of using a single cloned gene that determines post-translational capabilities, use is made of uncloned gene segments (high molecular weight genomic DNA, for example) or a library of cloned genomic DNA fragments or cDNA molecules. Transfected cells generated in this manner are then subjected to selection methods based upon detection of a newly-acquired desired post-translational capability, to isolate clonal cell lines expressing this capability.

In another embodiment, enzymes obtained in accordance with the present invention can be used in an in vitro reaction to modify cell-surface oligosaccharide molecules. For example, the inventor has purified the blood group A UDP-GalNAc transferase and its substrate UDP-GalNAc to convert in vitro blood group H oligosaccharide determinants on mouse cells to blood group A determinants (Ernst et al, J. Biol. Chem. (1989) 264:3436-3447). An analogous scheme can be employed using enzymes obtained in accordance with the present invention, alone, or in conjunction with other available glycosyltransferases and glycohydrolases, to modify existing cell surface oligosaccharide molecules on dead or viable, functional eukaryotic or prokaryotic cells, in such a manner as to render their cell surface oligosaccharide structures relevant for a desired characteristic.

Such a host cell possessing added specific post-translational glycosylation capability is used in accordance with known recombinant technology to obtain a glycoprotein, glycolipid, or free oligosaccharide. This cell being characterized by possessing both the post-translational glycosylation capability as well as the capability of producing the recombinant glycoprotein, glycolipid, or free oligosaccharide.

These latter embodiments can be used, for example, to add novel oligosaccharide molecules to the surface of specific kinds of mammalian cells (cells with specific or general immune function, for example) for the purpose of targeting them to particular tissues or other locations in the body, for a therapeutic or diagnostic use. In particular, such modified cells could then be targeted to tissues expressing lectin-like cell adhesion molecules that specifically recognize particular oligosaccharide structures that have been added to the surface of the modified cells.

In another embodiment, the present invention provides a method for suppression of glycosylation activity in a cell. In this embodiment, specific post-translational glycosylation capability is deleted from a host cell. This result can be achieved by introducing a specific cloned reagent into a cell by standard transformation or transfection techniques, after in vitro modifications that (i) inactivate the gene and (ii) insert with it or adjacent to it one or more genetically selectable markers. Introduction of this modified inactive gene effectively replaces the endogenous functional gene via homologous recombination, using standard techniques.

If necessary, two or more rounds of this process can be performed to inactivate both wild type genes in a diploid (or higher ploidy) organism. The end result is a cell line with (two) non-functional genes not now capable of determining the post-translational capability whose elimination was desired. Alternatively, the gene obtained in accordance with the present invention is introduced to a cell by transformation or transfection, in a state in which it is expressed in an anti-sense orientation, using standard techniques. This eliminates expression of its cognate wild type gene, via standard anti-sense expression methods. Treatment of the cell with anti-sense synthetic oligonucleotides, whose sequence(s) is (are) derived from the gene obtained in accordance with the present invention, can also be used to eliminate expression of the cognate wild type gene, again via standard methods.

Alternatively, the gene obtained in accordance with the present invention is introduced into a cell by transformation or transfection, such that the expression of a new post-translational modification prevents or eliminates expression of an undesired one. This approach turns on the observation that the actions of some glycosyltransferases on common acceptor substrates are mutually exclusive, i.e., α(1,2)fucosylation can prevent α(2,3)siaylation and vice versa, or α(1,3)galactosylation can prevent α(2,3)siaylation, and vice versa.

Addition or deletion of cellular post-translational capabilities (including glycosylation) allows, for example, the generation of host cell lines that can be used to produce lipids, proteins, or free oligosaccharides of diagnostic or therapeutic utility, whose specific post-translational modifications, including glycosylation, affect their function. For example, recombinant proteins such as tissue plasminogen activator or erythropoietin normally exists as glycoproteins. Should specific oligosaccharide structures on these glycoproteins be shown to have beneficial effects on their biosynthesis, serum half life, receptor interaction, or other function, the reagents and processes provided by the present invention can be used to construct hosts that yield recombinant proteins with the specific, and functionally optimal, oligosaccharide structures.

This embodiment can be used, for example, to delete specific oligosaccharide molecules from the surface of specific kinds of mammalian cells (cells with specific or general immune function, for example) for the purpose of preventing targeting to their normal, physiologic tissues or other locations in the body, and thus allow them to be targeted to other non-physiologic targets for therapeutic or diagnostic use. In particular, such modified cells can be shunted away from tissues where they normally act, to tissues expressing lectin-like cell adhesion molecules with specificities for other kinds of cells.

In another embodiment, the present invention provides gene products in heretofore unavailable amounts. These gene products, glycosyltransferase enzymes, can be used in enzymatic reactors to produce glycoproteins, glycolipids, oligosaccharides or polysaccharides of interest. In this embodiment, cloned glycosyltransferase gene segments can be used with standard recombinant protein expression systems to generate large amounts of the enzyme encoded by the gene. These enzymes can be used in bioreactors in in vitro, large scale, synthesis of oligosaccharides or glycolipids, or for glycosidic modification of proteins and glycoproteins.

Acceptor oligosaccharides in such a scheme can be derived from any of the following:

(a) commercially available mono-, di- or higher order saccharides prepared from natural sources, or by chemical synthesis;

(b) di- or higher order oligosaccharides produced in vitro by other recombinant enzymes generated by this process; or

(c) di- or higher order oligosaccharides produced by or purified from cell lines whose post-translational capabilities have been engineered as described above.

In this embodiment, two in vitro bioreactor-type approaches can be used. In one embodiment, an oligosaccharide acceptor and nucleotide sugar substrate are introduced into the reactor containing a solid phase matrix to which is bound catalytically active glycosyltransferase. This matrix can be generated using the fusion protein noted above which comprises a catalytically active moiety, as a soluble segment of the glycosyltransferase, fused to a protein segment that can be used to bind the fusion protein to a solid phase matrix. A specific example of such a fusion protein is a catalytically active segment of the mouse α(1,3) galactosyltransferase, fused to a segment of the IgG binding domain of Staph. protein A (Larsen et al, Proc. Natl. Acad. Sci. (USA), 86, 8227-8231, 1989).

Acceptor and nucleotide sugar substrates are incubated in such a reactor, at an appropriate temperature, pH, and other known conditions, for a length of time sufficient to yield a desired amount of oligosaccharide or polysaccharide product. The product is then harvested by using known techniques.

In the variation, the nucleotide sugar substrate and soluble glycosyltransferase catalytic domain-containing fusion protein is introduced into a reactor containing the oligosaccharide acceptor molecule covalently attached (i.e., immobilized) to a solid phase matrix. Attachment is carried out using known techniques in such a manner as to make available to the reaction medium the portion of the oligosaccharide acceptor molecule that will be enzymatically modified.

The present invention provides a method for generating animal cell lines with specific capabilities for post-translational modification of proteins produced by them, as well as a method to isolate cloned genes, cloned complementary cDNAs, and their mRNAs, that determine the expression or biosynthesis of the enzymes responsible for post-translational modification of eukaryotic (e.g. animal, such as mammalian) proteins, especially (but not limited to) those post-translational processes that construct eukaryotic (e.g. animal, such as mammalian) glycoconjugates, without the need to first isolate the protein products of these genes. This includes cloned genes, cloned complementary cDNAs, and their mRNAs, that encode eukaryotic (e.g. animal, such as mammalian) enzymes that post-translationally modify proteins by glycosylation and sulfation, as well as phosphorylation, methylation, fatty acylation, and removal of glycosyl modification (glycohydrolases).

The uses of the present invention thus include the following:

(i.) Construction of animal cell lines with specific post-translational capabilities (for the production of diagnostics and therapeutics).

This method can be used to construct animal cell lines that are suitable host cells for the production of diagnostic or therapeutic material whose usefulness or efficacy depends upon specific post-translational modifications. For example, the biological effectiveness of most therapeutic proteins or peptides, recombinant or otherwise, often depends critically upon the oligosaccharide structure(s) that are covalently attached to them. The structure of these oligosaccharides is primarily a function of the number and kind of glycosyltransferase enzymes that are found in the cell used to produce these therapeutic products.

Animal cells and yeasts are competent to perform these glycosylation reactions; however, not all glycosyltransferase enzymes are produced by every animal cell or yeast, and therefore, some oligosaccharide structures are not produced by them. The converse is also true, namely, that the producing cell may express some glycosyltransferases that create oligosaccharide structures which prevent effective bioactivity. The present invention provides for the creation or elimination of specific glycosyltransferases capabilities in producing cells, so that therapeutic effectiveness of products made by the cells may be optimized.

The old methods used for this process include an empirical approach to identify a cell line most appropriate for the production of the recombinant or natural product. This is generally not optimal since cell lines with suitable post-translation modification capabilities may not exist naturally, or may not be especially suited to high level production of an appropriately modified product. Alternatively, unwanted post-translational modifications present on a therapeutic material produced by an empirically identified animal cell line can be removed chemically or enzymatically, a process that may be costly or inefficient, or both.

The advantages of the present methods over the older methods include the ability to construct cell lines with specific post-translational modification capabilities; properly constructed, these cell lines eliminate any need for chemical or enzymatic treatment of a therapeutic or diagnostic material to remove unwanted post-translational modifications. Moreover, cell lines with inappropriate post-translational modification capabilities, but that are otherwise excellent cells for production, may be modified to achieve correct post-translational modification of the product.

This method allows the construction of animal cell lines with post-translational modification capabilities precisely tailored to the specific needs of a particular diagnostic or therapeutic product produced by animal cells.

(ii.) Isolation of reagents suitable for efficient enzymatic synthesis and production of oligosaccharides (in enzyme reactors, for example).

Oligosaccharides can have therapeutic utility as immunomodulatory reagents in the field of organ transplantation. In particular, soluble and solid-phase oligosaccharides may find use as therapeutic agents with wbich to block or ameliorate antibody-mediated organ transplant rejection in cases involving incompatibility due to differences in the major blood group antigen systems of the organ donor and the recipient. Similarly, soluble oligosaccharides can find use as therapeutic agents that function by blocking attachment of bacterial, viral, or parasitic pathogens to glycoconjugate receptors found on the surface of the animal tissues that these pathogens invade.

Moreover, glycoconjugates have been implicated in modulating adhesive events between cells and between cells and their environment during developmental and differentiation processes. These events included binding of spermatozoa to eggs, and the initial events that mediate attachment of fertilized ova to the uterine wall at the beginning of implantation. These observations show, for example, the possibility that contraceptive uses for (biologically "natural") oligosaccharide molecules exist.

Currently, oligosaccharides of defined structure are produced by chemical synthesis (a procedure that is inefficient and costly) or by isolation from natural sources (using costly and inefficient procedures that often require the processing of large quantities of animal or plant material, and the purification of the desired oligosaccharide from other contaminating oligosaccharides).

The present invention provides a mechanism for the isolation of cloned glycosyltransferase genetic sequences, which in turn can be used to economically synthesize abundant quantities of purified glycosyltransferase enzymes. These can be used to construct enzyme bioreactors (enzymes in solution or immobilized on a solid phase matrix) capable of enzymatic synthesis of these structures.

This is more efficient than approaches involving the chemical synthesis of oligosaccharides or their purification from natural sources, for a variety of reasons. One, the only chemicals necessary are the enzyme substrates; most of these are easily obtained or synthesized. Two, enzymatic synthesis produces only the desired product and the nucleotide monophosphate or nucleotide diphosphate product of substrate hydrolysis. These latter two chemicals are found as the natural by-products of these reactions in animal cells, are essentially non-toxic, and may be easily separated from the oligosaccharide synthetic product.

By contrast, chemical synthetic procedures typically generate numerous products of side reactions which must be removed, and which may be toxic as well. Similarly, purification of oligosaccharides from natural sources requires the removal of other contaminating oligosaccharides present in the natural material.

Three, enzymatic catalysis is extraordinarily efficient; virtually complete conversion of substrate to product can be achieved. By contrast, chemical synthesis of these structures is a multi-step process; yields at each step may be much much less than 100%, and the cumulative efficiency of current chemical synthesis procedures does not approach the efficiency possible with enzymatic synthesis. Similarly, purification of oligosaccharides from natural materials can entail significant losses inherent to the purification procedures required to separate the desired oligosaccharide from contaminating, irrelevant and/or undesirable oligosaccharides, with inefficient isolation of the desired oligosaccharide.

Although glycosyltransferases for synthetic use may be purified from animal tissues, these purifications are themselves inefficient, primarily because the enzymes are typically present in very low abundance. The present invention provides two mechanisms that provide for the abundant production of these enzymes.

First, this can be done through the construction and selection of animal cells that produce relatively large quantities of the enzymes. Alternatively, this invention provides a mechanism to isolate cloned cDNAs encoding these enzymes, or to construct synthetic genes that encode these enzymes via information derived from such cloned cDNAs or genes. These cloned nucleic acid sequences can then be used with standard recombinant DNA technologies to produce large quantities of glycosyltransferases.

(iii.) Isolation of reagents suitable for producing recombinant glycosyltransferases to be used directly as research reagents, or to be used to generate anti-glycosyltransferase antibodies for research applications.

The present invention provides two mechanisms for producing large quantities of these enzymes (see (ii.) above--i.e., specially constructed animal cells, or via natural or synthetic genes encoding these enzymes) which may be used as research tools with which to study the structures and functions of oligosaccharides and glycoproteins. Likewise, the enzymes produced by this method, or the nucleic acid sequence and derived protein sequence provided by this method, may be used to generate antibodies to these enzymes (via immunization with synthetic peptides whose sequences are derived from the cloned enzyme cDNAs or genes, or by direct immunization with the recombinant enzymes). These antibodies can also be used as research reagents to study the biosynthesis and processing of these enzymes, and can be used as an aid in their purification for all the uses described in this disclosure.

(iv.) Antibodies to glycosyltransferases as diagnostic reagents.

Some of these glycosyltransferases have been implicated as tumor markers in body fluids. The enzymes have typically been assayed in these fluids by activity assays, which may be subject to non-specificity due to competing glycosyltransferase activity. These assays may also be insensitive since it is possible that inactive enzymes might be useful as tumor markers but would not be detected by enzyme activity assays.

The present invention provides a mechanism for generating antibodies to these enzymes (monoclonal and polyclonal antibodies against synthetic peptides constructed from information derived from cloned glycosyltransferase cDNAs or genes, against enzymes produced by recombinant glycosyltransferases, or against enzymes produced by animal cells constructed by this method). Anti-glycosyltransferase antibodies specific for particular glycosyltransferases can be produced by this means, and can be used to detect and quantitate glycosyltransferases in body fluids with specificity and sensitivity exceeding enzyme activity assays.

(v.) Engineering of glycosyltransferase substrate specificity to generate novel glycoconjugate structures on secreted or cell-associated glycoconjugates.

The present invention provides reagents (cloned glycosyltransferase genes or cDNAs) and genetic selection methods that, when used with appropriate known mutagenesis schemes, allow the generation of mutant glycosyltransferases that generate glycosidic linkages different from that generated by the wild-type enzyme. These novel linkages may or may not be naturally occurring, and find utility as moieties that enhance bioactivity of the molecules to which they are attached. Alternatively, mutagenesis and selection approaches can be used to generate mutant enzymes that act in a dominant negative fashion. The dominant negative mutants so generated can be used to inactivate endogenous glycosyltransferase activities when the product(s) of such an enzyme are not desired.

This invention allows the isolation of glycosyltransferase genes (as well as genes that direct the synthesis of enzymes that perform the post-translational modifications) by methods designed to identify the surface-expressed product of the enzyme, and without the need to purify the enzyme as is required of standard molecular cloning procedures (i.e., without any information about the primary structure of the enzyme, and without antibodies directed against the enzyme).

A consequence of one implementation of this method is the generation of cells with specific capabilities for glycosylation. One version of the detailed implementation of this method is described in the following publications by the inventor, J. Biol. Chem. (1989) 264(6): 3436-3447 and J. Biol. Chem. (1989) 264(19): 11158-11167, both of which are herein incorporated by reference.

In outline, this version of the method entails the generation of cultured animal cell lines with specific abilities to construct desired glycoconjugate structures, by introducing exogenous genetic material into cells that do not express the desired glycosyltransferase or its product, using genetic material from cells that do express the desired enzyme. A positive selection procedure is then employed to identify transfected cells that express the enzyme product on the surface of the cell. The transfected genetic sequences responsible for this new phenotype are then isolated by standard procedures involving gene library construction and nucleic hybridization. This method allows the isolation of the genetic material determining expression of the glycosyltransferase without the need to purify the enzyme.

Although detection and isolation of these sequences by hybridization procedures involving a dispersed and repetitive human DNA sequence (Alu) is used to illustrate isolation of the gene, other methods may be used to "tag" transfected sequences, including but not limited to the ligation to the transfected DNA of DNA markers that allow identification and isolation of the desired genes by nucleic acid hybridization or genetic selection (supF or G418 resistance "Neo" sequences, for example) procedures. Three methods for the selection of transfected cells with the appropriate phenotype, flow cytometry, "resetting", and "panning", are described in Examples I, II, and III. Although an antibody specific for the enzyme product was used in the examples, other non-antibody reagents that specifically recognize surface expressed enzyme products may also be employed, including plant and animal lectins.

The enzymes provided by the present invention are imbued with certain unique characteristics, as compared to the corresponding native enzyme. Naturally-derived glycosyltransferases have been purified, with certain claims being made to the homogeneity of the product obtained. Nonetheless, such claims of homogeneity have been made based upon analyses of the preparations by SDS-polyacrylamide gel electrophoresis methods. In the older literature (i.e., pre-(1982) the homogeneous enzyme was identified in the gel by Coomassie blue staining, or other staining methods, that are notably less sensitive than contemporary silver staining approaches. It is thus almost certain that such preparations were less than homogeneous.

In more contemporary literature, three glycosyltransferases have been analyzed by silver staining methods (i.e., rat sialyl-T, GlcNAc-T-I and GlcNAc-T-II). These appear to be virtually free of contaminant proteins. Nonetheless the small amounts of final pure proteins obtained using these purification procedures were analyzed using the sensitive silver staining method, which is not sufficiently sensitive to detect levels of contaminants of roughly 5 to 10 wt. %, in the small amounts of pure protein available. Thus, prior to the present invention, glycosyltransferases having a level of purity of at least 95 wt. %, preferably at least 98 wt. % were not available. The present recombinant glycosyltransferases which are obtained using cloned glycosyltransferase DNA sequences, in large amounts, in soluble form, or fused to an affinity-purifiable protein segment, can be obtained in a truly, heretofore unavailable, homogeneous state.

The proteins provided by the present invention, as noted above, may also be distinguished from heretofore available proteins by the fact that they can be made in a non-glycosylated form. Many, if not all naturally-derived glycosyltransferases are glycoproteins, that is, they contain themselves one or more N-linked and/or O-linked oligosaccharide structures. These structures can themselves be glycosylated by the enzyme itself in an enzymatic reactor, for example, and this represents a competing acceptor substrate which could reduce the efficiency of the reaction and contribute to premature enzymatic activity loss. This "autoglycosylation" phenoma has the potential of either inactivating or reducing the catalytic efficiency of the enzyme and/or bioreactor.

Cloned glycosyltransferases provide a way to circumvent this problem. Firstly, expression of cloned glycosyltransferases in a bacteria host, such as E. coli, that is incapable of glycosylating these enzymes, will yield large amounts of non-glycosylated glycosyltransferases. These recombinant proteins can be used in a bioreactor, and since they are not themselves glycosylated, may be superior in performance to the naturally derived, glycosylated enzymes for the reasons noted above.

Alternatively, if it is necessary to express these enzymes in an eukaryotic cell host that is capable of glycosylating the recombinant enzyme, standard site-directed mutagenesis approaches can be used to eliminate from the recombinant protein the amino acid signals that allow animal cells to glycosylate the enzymes. These known signals include certain asparagine residues, falling within the N-X-T or N-X-S motif that allows asparagine-linked glycosylation, and also includes some serine and threonine residues that are substrates for O-linked glycosylation.

Standard mutagenesis methods can be used to alter the DNA sequence encoding the glycosyltransferase to either delete the codon that encodes these N, S or T residues, or change the respective codon to a codon that determines an amino acid with similar physical properties, but that is incapable of supporting N-linked or O-linked glycosylation.

The present invention also provides unique mutant recombinant glycosyltransferases. Isolation and expression of glycosyltransferase genes and cDNAs offers the opportunity to generate mutant glycosyltransferases with properties superior to the fixed properties inherent in the naturally occurring enzymes. Standard techniques based upon site-directed, or random mutagenesis, can be used to obtain mutant glycosyltransferases with some of the illustrative properties:

(1) Minimal catalytic domain: progressive deletion of amino acids from the glycosyltransferase protein can be achieved, and the resulting mutant glycosyltransferases can be tested for activity. Based upon known functions for different parts of these molecules, it can be predicted that a catalytically active mutant glycosyltransferase can be produced that is (a) soluble (lacks transmembrane segment on natural glycosyltransferases that render them insoluble and thus unsuitable for bioreactors), and (b) much smaller than the natural glycosyltransferase (which retains transmembrane segment and the "stem" region, neither of which are necessary for catalytic activity).

On a protein mass basis, small catalytically-active domains derived from mutated glycosyltransferase genes or cDNAs represent more catalytic activity than the larger, naturally occurring glycosyltransferases that carry along non-catalytically active transmembrane and/or stem region protein "baggage." Thus, the recombinant mutant-derived catalytic domain is much more efficient for use in vitro synthesis of oligosaccharides, and by a reactor for example.

Approaches to amplification of mRNA for glycosyltransferases:

The cell line used as a source of genetic material (mRNA for cDNA library construction, or genomic DNA for genomic library construction or genomic DNA transfection) for gene transfer to isolate a glycosyltransferase gene can be manipulated to optimize this process. Selection can be applied to this cell line to enhance steady state levels of the glycosyltransferase mRNA, and/or amplify its respective gene so that multiple copies of it exist in this donor cell. This can be done by subjecting the cell line (either after or without chemical, radiation, or other mutagenesis method) to a selection procedure that selects a variant of the cell that expresses higher amounts of the glycosyltransferase oligosaccharide product, at the surface of the cell, for example. This type of approach is illustrated in Example II.

Increased numbers of the oligosaccharide product molecules correlate with increased numbers of the cognate glycosyltransferase(s) enzyme molecules within the cell, and with an increase in steady state levels of the glycosyltransferase mRNA. Higher levels of this glycosyltransferase mRNA means that more copies of the respective cDNA will be present in a cDNA library prepared from the high-expression variant cell line, and thus will increase the likelihood of rescuing these glycosyltransferase cDNAs from the library. In some cases, higher levels of the specific mRNA can be associated with an increase in the number of copies of the cognate glycosyltransferase gene. Since such an amplified glycosyltransferase gene is more abundant in the cell's genome than other irrelevant genes, and more abundant than in a parental, non-selected cell line, it is easier to isolate by genomic DNA library or genomic DNA transfection approaches.

It can be shown by transfection studies that expression of some oncogenes can increase expression of some glycosyltransferases. Thus a cell line can be modified by transfection with one or more oncogenes, using standard transfection methods, and readily available vectors containing oncogenes, and resultant transfected clones can be assayed for increased giycosyltransferase levels. Such clones can also be identified or selected for by FACS or lectin selection methods outlined in Examples I, II, and III below. These clones can then be used for cDNA library preparation as noted above.

A number of chemical reagents have been shown to induce expression of, or increase expression of, glycosyltransferases, in cell lines. This may be associated with in vitro differentiation of the cell line, and such agents include retinoic acid, as well as dimethylsulphoxide-induced differentiation of human and mouse hematopoietic precursors, with concomittant increases in the expression of some glycosyltransferases. This occurs because of an increase in the steady state level of mRNA for the glycosyltransferase in question, and can be used to enhance the ability to isolate a cognate cloned cDNA using the cDNA library-mediated transfection approach (as shown in Example II, below).

An alternative approach for the isolation of genes or cloned cDNAs that encode animal glycosyltransferases (or other post-translational modification enzymes), by detecting the enzyme product at the cell surface, and without the need to purify the enzyme, is as follows: cDNA libraries are constructed in a plasmid or phage vector that will express the cloned cDNAs in a mammalian or yeast host, using mRNA prepared from cells or tissue that expressed the desired enzyme. This cDNA library is then screened for the desired cDNA by introducing the library into a host cell line that does not express significant amounts of the enzyme, nor its surface-expressed product, but that does have the necessary enzyme substrate molecules, and which is capable of displaying the enzyme's oligosaccharide product on its surface. The host cells which have taken up the cDNA library are subjected to selection for cells that contain the desired cDNA and thus express the new corresponding oligosaccharide product, using flow cytometry, resetting, or panning, and a reagent specific for the enzyme's oligosaccharide product. Cloned cDNAs that direct the expression of the desired enzyme may then be isolated from the selected cells by standard methods.

This approach may be used with the following techniques:

1. Stable transfection into animal cells, selection, followed by rescue of the desired clone cDNA by nucleic acid hybridization procedures, or by the COS cell fusion technique, depending on the vector used.

2. Transient transfection into COS or WOP cells, selection, followed by rescue of the desired clone cDNAs, by the method of Seed, Proc. Nat'l. Acad. Sci. (USA) (1987) 84:3365-3369, or similar methods that make use of cDNA cloning vectors that replicate as episomes in mammalian cells (i.e. Margolskee et al, Mol. Cell. Biol., (1988) 8:2837-2847.

3. Transformation of yeast cells, selection, followed by rescue of the desired cloned cDNA by nucleic acid hybridization procedures.

In addition, the mammalian cDNA expression libraries may also be screened by the sib selection method, using an enzyme assay to detect pools of cDNA molecules that contain cloned cDNAs encoding the relevant glycosyltransferase (or other enzyme involved in post-translational modification). Specifically, a cDNA library is constructed in a mammalian expression vector (plasmid or phage), using mRNA prepared from a cell that expresses the appropriate glycosyltransferase.

This library is then screened by first dividing it into pools of bacteria cells containing clones, each pool representing some fraction of the library, but the pools in aggregate representing the entire library. A portion of each pool is stored, and the remainder (containing sibs of the stored clones) is processed for cDNA-vector DNA molecules (i.e. plasmid or phage DNAs). The DNA prepared from each pool is separately introduced into one of the appropriate host cells described above (see 1, 2, and 3), and, after allowing for an appropriate expression time, extracts are prepared from the transfected or transformed host cells and these extracts are assayed for the appropriate glycosyltransferase activity.

The smallest pool that is thus found to contain plasmids directing the synthesis of the appropriate enzyme is retrieved from storage and subdivided. Again, a representative portion of these pools is stored, and the remainder (again containing sibs of the stored clones) of each is processed for plasmid DNA, transfection or transformation, expression, extract preparation, and enzyme assay. This process is repeated until single clones are isolated that direct expression of the relevant glycosyltransferase. Thus, this process does not rely upon surface expression of the enzyme product to isolate the appropriate cloned cDNA or gene. A version of this approach is presented in Example III.

The procedure used in the present invention need not be restricted by the genetic complement of the host; the gene transfer aspect of this invention allows expression of genes not normally expressed or even present in the recipient cell. Although the present text specifically illustrates application to glycosyltransferases, it may be applied to the enzymes and genes that control other forms of post-translational modification, including sulfation, phosphorylation, methylation, fatty acylation, and removal of glycosyl modification (glycohydrolases).

The method described to this point involves isolation of glycosyltransferase genes or cDNAs by selection for a dominant glycosylation trait. The transient expression system described for use in COS or WOP cells can also be used to identify and clone cDNAs homologous to glycosyltransferase transcripts already present in the COS or WOP host.

Specifically, cloned cDNAs transcribed in the "anti-sense" orientation may eliminate expression of the cognate glycosyltransferase in the COS or WOP host, resulting in a recessive glycosylation trait. These DNA sequences can then be isolated by selection for surface expression of the oligosaccharide linkage recognized by the glycosyltransferase whose expression was eliminated, again by the procedures described below (flow cytometry, "resetting", and "panning"), as detailed in Examples I, II, and III. Alternatively, the sib selection approach might be used to identify cloned cDNA molecules that decrease or eliminate the expression of an endogenous glycosyltransferase, as determined by enzyme assays.

The DNA sequences and corresponding glycosyltransferases of the present invention are summarized in Table

                                      TABLE 1     __________________________________________________________________________     A.       Fuc-TIII (Lewis enzyme), SEQ ID NO: 1 (DNA) and SEQ ID NO: 2 (protein)       DNA at least nucleotide positions 199 through 1158 of SEQ ID NO: 1, and           up to the whole of SEQ ID NO: 1       Protein           at least amino acid positions 43 to 361 of SEQ ID NO: 2, and up to           the whole of SEQ ID NO: 2     B.       Murine α(1,3)galactosyltransferase, SEQ ID NO: 3 (DNA) and SEQ ID       NO: 4 (protein)       DNA at least nucleotide positions 463 through 1461 of SEQ ID NO: 3, and           up to the whole of SEQ ID NO: 3       Protein           at least amino acid positions 63 to 394 of SEQ ID NO: 4, and up to           the whole of SEQ ID NO: 4     C.       Human H α(1,2)fucosyltransferase, SEQ ID NO: 5 (DNA) and SEQ ID       NO: 6 (protein)       DNA at least nucleotide positions 4782 through 5783 of SEQ ID NO: 5,           and up to the whole of SEQ ID NO: 5       Protein           at least amino acid positions 33 to 3365 of SEQ ID NO: 6, and up to           the whole of SEQ ID NO: 6     D.       Fuc-TIV, SEQ ID NO: 7 (DNA) and SEQ ID NO: 8 (protein)       DNA at least nucleotide positions 2089 through 3159 of SEQ ID NO: 7,           and up to the whole of SEQ ID NO: 7       Protein           at least amino acid positions 50 to 405 of SEQ ID NO: 8, and up to           the whole of SEQ ID NO: 8     E.       Fuc-TV, SEQ ID NO: 10 (DNA) and SEQ ID NO: 11 (protein)       DNA at least nucleotide positions 247 through 1111 of SEQ ID NO: 10,           and up to the whole of SEQ ID NO: 10       Protein           at least amino acid positions 43 to 374 of SEQ ID NO: 11, and up to           the whole of SEQ ID NO: 11     F.       Fuc-TVI, SEQ ID NO: 13 (DNA) and SEQ ID NO: 14 (protein)       DNA at least nucleotide positions 255 through 1208 of SEQ ID NO: 13,           and up to the whole of SEQ ID NO: 13       Protein           at least amino acid positions 43 to 359 of SEQ ID NO: 14, and up to           the whole of SEQ ID NO: 14     __________________________________________________________________________

SEQ ID NO:1 encodes a protein sequence termed Fuc-TIII capable of functioning as a GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNAc(/Glc) α(1,3/1,4)-fucosyltransferase. This protein is an enzyme that can be used to construct the oligosaccharide "ligand" for Endothelial Leukocyte Adhesion Molecule-1(ELAM-1) that is disclosed in Applicant's co-pending U.S. patent application Ser. No. 07/603,018, filed Oct. 25, 1990, which is hereby incorporated by reference. This ligand is the sialyl-Lewis x molecule. Also, this enzyme, when expressed by the cloned DNA sequence described here, functions within mammalian cells to generate de novo expression of specific cell surface glycoconjugate structures on those cells. These structures are recognized by antibodies against the following cell surface glycoconjugate structures (See FIG. 8 and Table 2).

                  TABLE 2     ______________________________________     SSEA-1 or Lewis x                  Galβ(1,4) Fucα(1,3)!GlcNAc     sialyl-Lewis x                  NeuAcα(2,3)Galβ(1,4) Fucα(1,3)!GlcNAc     Lewis a      Galβ(1,3) Fucα(1,4)!GlcNAc     sialyl-Lewis a                  NeuAcα(2,3)Galβ(1,3) Fucα(1,4)!GlcNAc.     ______________________________________

In the above DNA sequence; (I), the sequence corresponding from amino acid position 43 to amino acid position 361 is functional, but a larger sequence of up to the whole sequence shown can be used.

This enzyme, when expressed by the cloned DNA sequence described here, functions in the enzymatic manner indicated in its name, when assayed in extracts prepared from cells that express the DNA sequence (See Table 4). The oligosaccharide product of this enzyme represents fucose linked in alpha 1,3 configuration to neutral or α(2,3) sialylated "type II" acceptors, or fucose linked in alpha 1,4 configuration to neutral or α(2,3) sialylated "type I" acceptors as shown below:

    ______________________________________     SSEA-1 or Lewis x                  Galβ(1,4) Fucα(1,3)!GlcNAc     sialyl-Lewis x                  NeuAcα(2,3)Galβ(1,4) Fucα(1,3)!GlcNAc     Lewis y      Fucα(1,2)Galβ(1,4) Fucα(1,3)!GlcNAc     Lewis a      Galβ(1,3) Fucα(1,4)!GlcNAc     sialyl-Lewis a                  NeuAcα(2,3)Galβ(1,3) Fucα(1,4)GlcNAc     Lewis b      Fucα(1,2)Galβ(1,3) Fucα(1,4)!GlcNAc.     ______________________________________

Throughout the remainder of this text, these products will be referred to as sub-terminal α(1,3) and α(1,4) fucose residues.

The catalytic domain of this enzyme has also been localized by expression studies. The enzymatic properties of the enzyme encoded by this cDNA, and chromosomal localization studies, indicate that this cDNA is the product of the human Lewis blood group locus.

This DNA sequence and the corresponding protein have the following uses:

(i.) Construction of animal cell lines with specific capabilities with respect to post-translational modification of the oligosaccharides on cell-surface, intracellular, or secreted proteins or lipids by sub-terminal α(1,3) and α(1,4) fucose residues that represent the products of this enzyme (for the production of diagnostics and therapeutics).

Specifically, the present cloned DNA sequence can be introduced by standard technologies into a mammalian cell line that does not normally express the cognate enzyme or its product (sub-terminal α(1,3) and α(1,4) fucose residues on oligosaccharides), and transcribed in that cell in the "sense" direction, to yield a cell line capable of expressing sub-terminal α(1,3) and α(1,4) fucose residues on oligosaccharides on cell-surface, intracellular, or secreted glycoproteins or lipids.

Alternatively, this cloned DNA sequence may be introduced by standard technologies into a mammalian cell line that does express the cognate enzyme and its product (sub-terminal α(1,3) and α(1,4) fucose residues), and transcribed in that cell in the "anti-sense" direction, to yield a cell line incapable of expressing sub-terminal α(1,3) and α(1,4) fucose residues on cell-surface, intracellular, or secreted glyco-proteins or lipids.

Alternatively, the endogenous GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNAc(/Glc)α(1,3/1,4)-fucosyltransferase gene(s), in a mammalian cell expressing the cognate enzyme(s), can be inactivated with the DNA sequence described here by homologous recombination techniques, or by "anti-sense" gene expression or oligonucleotide approaches based upon the DNA sequence described herein, or by dominant negative mutant fucosyltransferase sequences that inactivate endogenous GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNAc(/Glc)α(1,3/1,4)-fucosyltransferase(s) and that may be derived via mutagenesis and genetic selection schemes, in conjunction with the sequence information in this text.

This method can be used to construct animal cell lines that are suitable host cells for the production of diagnostic or therapeutic materials whose usefulness or efficacy depends upon the specific post-translational modification determined by this cloned DNA sequence and its cognate enzyme. For example, it is known that the biological effectiveness of many therapeutic proteins or peptides, recombinant or otherwise, may depend critically upon the oligosaccharide structure(s) that are covalently attached to them. The structure of these oligosaccharides is primarily a function of the number and kind of glycosyltransferase enzymes that are found in the cell used to produce these therapeutic products.

Animal cells and yeasts are competent to perform these glycosylation reactions; however, not all glycosyltransferase enzymes are produced by every animal cell or yeast, and therefore, some oligosaccharide structures (including sub-terminal α(1,3) and α(1,4) fucose residues generated by the enzyme encoded by the DNA sequence described here) are not produced by them.

The converse is also true, namely, that producing cells may express a glycosyltransferase analagous to, or identical to, the GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNAc(/Glc)α(1,3/1,4)-Fucosyltransferase encoded by the DNA sequence described here. It is likely that sub-terminal α(1,3) and α(1,4) fucose residues alter the bioactivity (for better or for worse) of natural or recombinant therapeutic or diagnostic agents (glycoproteins or glycolipids) produced by mammalian or other eukaryotic hosts. Eukaryotic host cells that are used to produce these recombinant agents can be altered with the DNA sequence information and related information described in this invention, to add subterminal α(1,3) and α(1,4) fucose residues to the oligosaccharides on recombinant product by expressing all or part of the cloned sequences described here in the desired host. Alternatively, sub-terminal α(1,3) and α(1,4) fucose residues may be eliminated from the product produced in these host cells by the use of transfected "anti-sense" vector constructs, recombination-based gene inactivation, "anti-sense" oligonucleotide approaches, or dominant negative mutant fucosyltransferases, outlined above.

The old "methods" used for this process include an empirical approach to identify a cell line that does or does not express this particular enzyme or an enzyme that functions in a similar or identical manner, for the production of the appropriately modified recombinant or natural product. This is not always optimal since cell lines with this particular post-translation modification capabilities may not exist naturally, or may not be especially suited to high level production of an appropriately modified product. Alternatively, unwanted sub-terminal α(1,3) and α(1,4) fucose residues present on a therapeutic material produced by an empirically identified animal cell line must be removed chemically or enzymatically, a process that may be costly or inefficient.

The advantages of using the cloned, functional DNA sequence described here in conjuntion with the technologies outlined above, relative to these older methods, include the ability to construct lines that specifically lack the capability to generate sub-terminal α(1,3) and α(1,4) fucose residues on the oligosaccharides of glycoproteins and glycolipids; properly constructed, these cell lines will eliminate any need for chemical or enzymatic treatment of a therapeutic or diagnostic material to remove unwanted sub-terminal α(1,3) and α(1,4) fucose residues. Moreover, in the event that sub-terminal α(1,3) and α(1,4) fucose residues are found to be desirable for a particular diagnostic or therapeutic product produced by animal cells, cell lines may be engineered with the cloned DNA sequence described here to generate these residues.

(ii.) Isolation of reagents suitable for efficient enzymatic synthesis and production of oligosaccharides (in enzyme reactors, for example).

Oligosaccharides have therapeutic utility as immunomodulatory reagents in the field of organ transplantation. In particular, soluble and solid-phase oligosaccarides find use as therapeutic agents with which to block or ameliorate antibody-mediated organ transplant rejection in cases involving incompatibility due to differences in the major blood group antigen systems of the organ donor and the recipient, including the Lewis blood group system. Likewise, soluble oligosaccharides may find use as therapeutic agents that function by blocking attachment of bacterial, viral, or parasitic pathogens to glycoconjugate "receptors" found on the surface of the animal tissues that these pathogens invade.

For example there is evidence that portions of the Lewis blood group oligosaccharide antigen (containing sub-terminal α(1,3) and α(1,4) fucose residues) serve as "receptors" for some forms of uropathogenic bacteria. Moreover, glycoconjugates, including subterminal α(1,3) and α(1,4) fucose residues, have been implicated in modulating adhesive events between cells, like leukocyte-ELAM-1 interactions, and between cells and their environment during developmental and differentiation processes. These events include binding of spermatozoa to eggs, and the initial events that mediate attachment of fertilized ova to the uterine wall at the beginning of implantation. These observations suggest, for example, the possibility that contraceptive uses for (biologically "natural") oligosaccharide molecules might exist. Oligosaccharide molecules constructed by this enzyme can disrupt leukocyte-ELAM interactions and thus function as anti-inflammatory agents.

Currently, oligosaccharides containing subterminal α(1,3) and α(1,4) fucose residues are produced by chemical synthesis (a procedure that is inefficient and costly or both) or by isolation from natural sources (using costly and inefficient procedures that often require the processing of large quantities of animal or plant material, and the purification of the desired oligosaccharide from other contaminating oligosaccharides).

The invention described here provides a mechanism to synthesize abundant quantities of purified GDP-Fuc: β-D-Gal(1,4/1,3)3-D-GlcNAc(/Glc)α(1,3/1,4)-Fucosyltransferase, Fuc-TIII. This can be used to construct an enzyme bioreactor (enzyme in solution or immobilized on a solid phase matrix, for example via the protein-A moiety fused to the catalytic domain of the enzyme (as described in Kukowska-Latallo et al, Genes Devel., (1990) 4:1288-1303) capable of enzymatic synthesis of structures containing sub-terminal α(1,3) and α(1,4) fucose residues.

This is more efficient than approaches involving chemical synthesis of structures containing sub-terminal α(1,3) and α(1,4) fucose residues or their purification from natural sources, for a variety of reasons. One, the only chemicals necessary are the enzyme substrates and co-factors; these are easily obtained or synthesized. Two, enzymatic synthesis of such structures will produce only the desired product and the nucleotide diphosphate product of substrate hydrolysis. This latter chemical is found as the natural by-product of these reactions in animal cells, is relatively non-toxic, and may be easily separated from the oligosaccharide synthetic product. By contrast, chemical synthetic procedures typically generate numerous products of side reactions which must be removed, and which may be toxic as well. Similarly, purification of oligosaccharides from natural sources requires the removal of other contaminating oligosaccharides present in the natural material.

Three, enzymatic catalysis is extraordinarily efficient; nearly or totally complete conversion of substrate to product might be achieved. By contrast, chemical synthesis of sub-terminal α(1,3) and α(1,4) fucose residues on oligosaccharides is a multi-step process; yields at each step may be much less than 100%, and the cumulative efficiency of current chemical synthesis procedures does not approach the efficiency possible with enzymatic synthesis. Similarly, purification of oligosaccharides with sub-terminal α(1,3) and α(1,4) fucose residues from natural materials can entail significant losses inherent to the purification procedures required to separate the desired oligosaccharide from contaminating, irrelevant oligosaccharides, with inefficient isolation of the desired oligosaccharide.

Although the GDP-Fuc: β-D-Gal(1,4/1,3) !-D-GlcNAC(/Glc) α(1,3/1,4)-fucosyltransferase encoded by the DNA sequence described here may be isolated from animal tissues for synthetic use, these purifications are themselves inefficient, primarily because the enzyme is typically present in very low abundance.

This invention provides two mechanisms that provide for the abundant production of this enzyme. First, this may be done through the construction and selection of animal cells that produce relatively large quantities of the enzymes. Alternatively, this cloned nucleic acid sequence may be used with standard recombinant DNA technologies to produce large quantities of the fucosyltransferase in yeasts or in prokaryotic hosts. Furthermore, the sequence encoding this enzyme may be modified via standard molecular cloning schemes or mutagenesis to yield a recombinant fucosyltransferase with novel properties that make it more desirable than the wild-type enzyme.

For example, modifications can be made to the enzyme that make it more stable, or more suitable for immobilization in a bioreactor.

(iii.) Isolation of reagents suitable for producing recombinant GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-fucosyltransferase to be used directly as a research reagent, or to be used to generate antibodies against the GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNAc(/Glc) α(1,3/1,4)-fucosyltransferase, for research applications.

This invention provides two mechanisms for producing large quantities of this enzyme (see ii. above--i.e., specially constructed animal cells, or via natural or synthetic genes encoding these enzymes) which may be used as a research tool with which to study the structures and functions of oligosaccharides and glycoproteins. Likewise, the enzyme produced by this method, or the nucleic acid sequence and derived protein sequence provided by this method, may be used to generate antibodies to this enzyme (via immunization with synthetic peptides whose sequences are derived from the cloned gene(s) or cDNA(s), or by immunization with the recombinant enzyme itself). These antibodies might also be used as research reagents to study the biosynthesis and processing of these enzymes, and can be used as an aid in their purification for all the uses described in this text.

(iv.) Antibodies to glycosyltransferases as diagnostic reagents.

Aberrant expression of GDP-Fuc:(β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-fucosyltransferase has been associated with malignancy in humans, suggesting that this enzyme might serve as a tumor marker for early detection of malignancy involving a number of human tissues. Enzyme tumor markers have typically been assayed in body fluids by activity assays, which may be subject to non-specificity due to competing or similar glycosyltransferase activity. These assays may also be insensitive since it is possible that inactive enzymes might be useful as tumor markers but would not be detected by enzyme activity assays.

This invention provides a mechanism for generating antibodies to this enzyme (monoclonal and polyclonal antibodies against synthetic peptides constructed from information derived from cloned DNA sequence encoding GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-fucosyltransferase, or against the recombinant enzyme produced by eukaryotic or prokaryotic hosts). Antibodies specific for this GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-fucosyltransferase produced could be used to detect and quantitate this glycosyltransferase in body fluids, with specificity and sensitivity exceeding enzyme activity assays, and with the possibility of serving as a tumor marker for early detection of malignancy.

(v.) Recombinant enzyme for use in screening natural and synthetic compounds for fucosyltransferase inhibitors or inactivators.

A number of studies have noted an association between increased numbers of cell surface sub-terminal α(1,3) and α(1,4) fucose residues on oligosaccharides of a cell and the ability of that cell to metastasize in a malignant fashion. If there is a causal relationship, then drugs that inhibit the enzyme encoded by the sequence in this text could be active as anti-tumor agents. Likewise, numerous recent studies have implicated sialylated and neutral oligosaccharides containing subterminal α(1,3) and α(1,4) fucose linkages in mediating adhesion of leukocytes to the selectin adhesion molecules (ELAM-1; GMP-140; Mel14/LAM-1) during inflammation. These studies suggest that molecules capable of preventing synthesis of α(1,3) and α(1,4) fucose linkages on leukocytes may thus function to diminish or even eliminate the ability of leukocytes to synthesize and display subterminal α(1,3) and α(1,4) fucose linkages, and would thus represent anti-inflammatory pharmaceutical agents. The reagents described in this text are useful for screening to isolate compounds or identify compounds that exhibit antifucosyltransferase activity, since the cloned sequence may be used with standard techniques to produce relatively large amounts of pure fucosyltransferase. This further aids in screening since the effects of potential inhibitors will be tested on a pure enzyme, without the confounding effects that may occur in whole cell extracts or with partially purified enzyme.

(vi.) Engineering of glycosyltransferase substrate specificity to generate novel glycoconjugate structures on secreted or cell-associated glycoconjugates.

This invention provides a reagent a) cloned GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-Fucosyltransferase cDNA) and the genetic selection method used to isolate it, that, when used with appropriate mutagenesis schemes, may allow the generation of mutant GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-Fucosyltransferases that generate glycosidic linkages different from that generated by the wild-type enzyme. These novel linkages may or may not be naturally occurring, and might find utility as moieties that enhance bioactivity of the molecules to which they are attached.

Alternatively, mutagenesis and selection approaches may be used to generate mutant GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-Fucosyltransferases that act in a dominant negative fashion. The dominant negative mutants so generated can be used to inactivate endogenous glycosyltransferase activities when the product(s) of such an enzyme are not desired. Mutant GDP-Fuc: β-D-Gal(1,4/1,3)!-D-GlcNac(/Glc) α(1,3/1,4)-Fucosyltransferases can also be generated, for example, that function as fucosidases that hydrolyze various sugar linkages (fucose, mannose, or others) from oligosaccharides in vitro and in vivo.

(vii.) Genotyping individuals for the Lewis locus.

DNA sequence polymorphisms within or linked to the gene corresponding to this cloned cDNA may be used to genotype individuals for the Lewis locus. This can find utility with respect to organ transplantation procedures, or as a measure of susceptibility to infections caused by pathogens that may use blood group structures as receptors for invasion (as in urinary tract infections, for example).

SEQ ID NO:3 encodes a mouse UDP-Gal:β-D-Gal(1,4)-D-GlcNAc α(1,3)-galactosyltransferase. SEQ ID NO:5 encodes a human GDP-Fuc:β-D-galactoside α(1,2)-fucosyltransferase. The uses for each of these proteins are generally the same as those discussed herein for the enzyme of SEQ ID NO:1.

Specific application of the enzyme encoded by SEQ ID NO:5 include enzymatic fucosylation of chain-terminating galactose residues on lactoseamine or neolacto type β-D-galactoside to α-2-L-fucose residues. Such modification can be performed in vitro using the purified α(1,2)FT, or its derivatives and its substrate GDP-fucose, using asialoglycans terminating with β-D-galactoside residues. Such asialoglycans exist naturally, and can also be constructed from glycans with terminal galactose moieties substituted with sialic acid by in vitro digestion with neuraminidase. Likewise, such fucosylation can be expected to occur when glycans are expressed in mammalian cells that have been transfected with the α(1,2)FT cDNA or gene segment. Such α(1,2) fucosylated glycans may have increased solubility properties, may have prolonged plasma half lifes (by virtue of the fact that the terminal galactose residues that normally mediate glycoprotein clearance by the asialoglycoprotein receptor of the liver are now covered up by fucose residues), differentiate from natural glycoforms, and may enhance bioactivity.

Molecular mechanisms used by cells to regulate the precise tissue-specific and developmental expression patterns of oligosaccharide structures are poorly understood. Such patterns however are probably determined largely by the coordinate regulation of expression of cognate glycosyltransferases. Since many of these enzymes recognize identical nucleotide sugar substrates or oligosaccharide acceptor substrates, it can be expected that they exhibit substantial primary protein and nucleic acid sequence similarities that would facilitate isolation of related glycosyltransferase genes by cross hybridization strategies.

Molecular cloning efforts by the inventor have allowed the isolation of several cloned glycosyltransferase cDNAs discussed above. Comparisons of the primary sequences of these enzymes reveals that they maintain virtually identical predicted structural topologies.

With the exception of one pair of distinct glycosyltransferases, however, there appear to be no substantial primary sequence similarities between these enzymes, even though many of them exhibit nucleotide sugar substrate or oligosaccharide acceptor substrate requirements that are virtually identical. The exceptional pair, a murine α1,3 galactosyltransferase sequence, or its human pseudogene homologue, and a human α1,3N-acetylgalactosaminide transferase share substantial primary protein and nucleic acid sequence similarity, even though these enzymes use different nucleotide sugar substrates and exhibit distinct oligosaccharide acceptor substrate requirements.

Taken together, these observations indicate that some glycosyltransferases may be structurally related, but such relationships cannot necessarily be predicted from knowledge of nucleotide sugar or oligosaccharide acceptor substrate requirements.

As noted above, the gene transfer procedure of the invention has been used by the inventor to isolate a cloned cDNA that encodes the human Lewis blood group fucosyltransferase. This enzyme is an exceptional glycosyltransferase in that it can catalyze two distinct transglycosylation reactions. Sequence comparisons of this enzyme with the blood group H α(1,2)fucosyltransferase indicates that these two fucosyltransferases maintain distinct primary sequences despite the fact that they use the identical nucleotide sugar substrate GDP-fucose, and can each utilize oligosaccharide acceptor molecules that terminate with unsubstituted type I or type II disaccharide moieties.

Biochemical and genetic data indicate that the human genome contains one or more structural genes that encode fucosyltransferases competent to construct surface localized SSEA-1 determinants. These enzymes are thought to be polypeptides distinct from the Lewis fucosyltransferase because they exhibit different acceptor substrate specificities and differential sensitivities to divalent cation and N-ethylmaleimide inactivation. Moreover, their expression is determined by loci distinct from the Lewis blood group fucosyltransferase locus, and they display tissue specific patterns that are distinct from the Lewis locus patterns.

Because these enzymes exhibit properties that are very similar to the Lewis blood group fucosyltransferase, the inventor recognized that it was possible that these enzymes and their corresponding genes might be sufficiently related at the primary sequence level to be able to isolate them by crosshybridization approaches. In another embodiment, the invention therefore provides a method for isolating a gene encoding a glycosyltransferase by cross-hybridization. The cross-hybridization techniques which can be used in accordance with the invention are generally known. See, e.g., Lauer et al. Cell (1980) 20:119-130, Fritsch et al, Cell (1980) 19:959-972, Haynes et al, J. Biol. Chem. (1980) 255:6355-6367, and Proudfoot et al, Proc. Nat. Acad. Sci. (USA) (1979) 76:5425-5439, all of which are hereby incorporated by reference.

As noted above, oligosaccharides constructed by animal cells are remarkable for their structural diversity. This diversity is not random but rather consists of specific sets of oligosaccharide structures that exhibit precise tissue-specific and developmental expression patterns. Molecular mechanisms used by cells to regulate these expression patterns are poorly understood. It can be expected, however, that such patterns are determined largely by the coordinate regulation of expression of the glycosyltransferases that determine these patterns. Recent molecular cloning efforts have allowed the isolation of several cloned glycosyltransferase cDNAs. Comparisons of the primary sequences of these enzymes have revealed that they maintain virtually identical predicted structural topologies. With the exception of one pair of distinct glycosyltransferases, however, there appear to be no substantial primary sequence similarities between these enzymes, even though many of them exhibit nucleotide sugar substrate or oligosaccharide acceptor substrate requirements that are virtually identical. The exceptional pair, a murine α1,3galactosyltransferase sequence, or its human pseudogene homologue, and a human α1,3N-acetylgalactosaminide transferase share substantial primary protein and nucleic acid sequence similarity, even though these enzymes use different nucleotide sugar substrates and exhibit distinct oligosaccharide acceptor substrate requirements. Taken together, these observations suggest that some glycosyltransferases may be structurally related, but such relationships cannot necessarily be predicted from knowledge of nucleotide sugar or oaigosaccharide acceptor substrate requirements. The inventor has recently used a mammalian gene transfer procedure to isolate a cloned cDNA that encodes the human Lewis blood group fucosyltransferase (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990). This enzyme is an exceptional glycosyltransferase in that it can catalyze two distinct transglycosylation reactions. Sequence comparisons of this enzyme with the blood group H α(1,2)fucosyltransferase (Larsen et al, Proc. Natl. Acad. Sci. USA, 87:6674-6678, 1990) indicates that these two fucosyltransferases maintain distinct primary sequences despite the fact that they use the identical nucleotide sugar substrate GDP-fucose, and can each utilize oiigosaccharide acceptor molecules that terminate with unsubstituted type I or type II disaccharide moieties (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990; Larsen et al, Proc. Natl. Acad. Sci. USA, 87:6674-6678, 1990). Biochemical and genetic data indicate that the human genome contains one or more structural genes that encode fucosyltransferases competent to construct surface localized SSEA-1 determinants (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990; Potvin et al, J. Biol. Chem., 265:1615-1622, 1990). These enzymes are thought to be polypeptides distinct from the Lewis fucosyltransferase because they exhibit different acceptor substrate specificities and differential sensitivities to divalent cation and N-ethylmaleimide inactivation (Potvin et al, J. Biol. Chem., 265:1615-1622, 1990). Moreover, their expression is determined by loci distinct from the Lewis blood group fucosyltransferase locus, and they display tissue specific patterns that are distinct from the Lewis locus patterns (Watkins, Adv. Hum. Genet., 10:1-116, 1980). Because these enzymes exhibit properties that are very similar to the Lewis blood group fucosyltransferase, the inventor considered it possible that enzymes and their corresponding genes might be sufficiently related at the primary sequence level to be able to isolate them by cross-hybridization approaches. The inventor has now achieved the isolation of several such cross-hybridizing human genes, an analysis of their structures, their expression in COS-1 cells after DEAE-dextran-mediated transfection, and analysis of their acceptor substrate properties.

Thus, in another embodiment, the invention provides DNA sequence SEQ ID NO:7 (set forth in FIG. 4) that encodes a protein sequence capable of functioning as a GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase, Fuc-TIV. This enzyme, when expressed by the cloned DNA sequence SEQ ID NO:7 functions within mammalian cells to generate de novo expression of specific cell surface glycoconjugate structures of those cells.

Present DNA sequence SEQ ID NO:9 (set forth in FIG. 5 wherein it is indentified as pFT-3 DNA) is comprised within DNA sequence SEQ ID NO:7. Namely, sequence SEQ ID NO:9 is found beginning at nucleotide position 1942 (the codon at nucleotide position 1942-1944) in sequence SEQ ID NO:7.

The DNA sequence and corresponding peptide provided in this embodiment of the invention must correspond at least to the segment from nucleotide position 2089 to 3159, preferably positions 1942 to 3156, of Sequence SEQ ID NO;7 set forth in FIG. 4. These DNA sequences, can have further DNA sequence attached optionally to each end. These pendent DNA sequences can be of any length and up to a length corresponding to that set forth in FIG. 4.

In a preferred embodiment, this embodiment of the invention provides DNA sequences, and their corresponding proteins, corresponding at least to the sequence between nucleotide positions 2089 to 3159, preferably positions 1942 to 3156, SEQ ID NO:7 and having attached to each end, optionally, further DNA sequences corresponding to those set forth in FIG. 4. In this case, the pendent DNA sequences and corresponding proteins, can be of any length and up to the length set forth in FIG. 4.

These glycoconjugate structures, constructed in part by this enzyme, are recognized by an antibody against the stage specific embryonic antigen I (SSEA-1 or Lewis x; structure Galβ(1,4) Fuc α(1,3)!GlcNAc), and by an antibody against the VIM-2 determinant NeuAcα(2,3)Galβ(1,4)GlcNAcβ-(1,3)Galβ(1,4) Fucα(1,3)!GlcNAc. This enzyme when expressed by DNA SEQ ID NO:7 functions in the enzymatic manner implied in its name, when assayed in extracts prepared from cells that express the DNA sequence, as illustrated in FIG. 8 and Table 2.

The oligosaccharide products of this enzyme represents fucose linked in alpha 1,3 configuration to the GlcNac residue of a "type II", lactos amine! acceptor. Throughout the remainder of this disclosure, these products will be referred to as subterminal α(1,3) fucose residues.

The isolation of three specific such cross-hybridizing human genes (SEQ ID NO:7, SEQ ID NO:10, SEQ ID NO:13), an analysis of their structure, their expression in COS-1 cells after DEAE-dextran-mediated transfection, and analysis of their acceptor substrate properties is described in the examples below, (Examples IV, V, and VI), and summarized in FIGS. 8 and Table 2. SEQ ID NO:10 (Fuc-TV) encodes a specific protein sequence capable of functioning as a GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)fucosyltransferase. This enzyme, when expressed by the cloned DNA sequence described, functions within mammalian cells to generate de novo expression of specific cell surface glycoconjugate structures on those cells. These structures are recognized by an antibody against the stage specific embryonic antigen I (SSEA-1 or Lewis x; structure Galβ(1,4)(Fucα(1,3)!GlcNAc), and by an antibody against the sialyl-Lewis x determinant NeuAcα(2,3)Galβ(1,4) Fucα(1,3)!GlcNAc. This enzyme, when expressed by the cloned DNA sequence described, also functions in the enzymatic manner implied in its name, when assayed in extracts prepared from cells that express the DNA sequence. The oligosaccharide products of this enzyme represent fucose linked in alpha 1,3 configuration to the GlcNac residue of a "type II" lactos amine! acceptor. Throughout the remainder of this disclosure, these products will be referred to as subterminal α(1,3) fucose residues. The location of the catalytic domain of this enzyme has been shown experimentally to encompass amino acids 43 to 374 of SEQ: ID NO:11.

The DNA and encoded protein of SEQ ID NO:13 and SEQ ID NO:14 (Fuc-TVI) may be used as follows:

i. Construction of animal cell lines with specific capabilities with respect to post-translational modification of the oligosaccharides on cell-surface, intracellular, or secreted proteins or lipids by sub-terminal α-(1,3) fucose residues that represent the products of this enzyme (for the production of diagnostics and therapeutics by the biotechnology industry).

Specifically, the cloned DNA sequence described here may be introduced by standard technologies into a mammalian cell line that does not normally express the cognate enzyme or its product (sub-terminal α(1,3)fucose residues on oligosaccharides), and transcribed in that cell in the "sense" direction, to yield a cell line capable of expressing sub-terminal α(1,3) fucose residues residues on oligosaccharides on cell-surface, intracellular, or secreted proteins or lipids. Altematively, this cloned DNA sequence may be introduced by standard technologies into a mammalian cell line that does express the cognate enzyme and its product (subterminal α(1,3) fucose residues), and transcribed in that cell in the "antisense" direction, to yield a cell line incapable of expressing sub-terminal α(1,3) and fucose residues on cell-surface, intracellular, or secreted proteins or lipids. Alternatively, the endogenous GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase gene(s), in a mammalian cell expressing the cognate enzyme(s), might be inactivated with the DNA sequence described here by homologous recombination techniques, or by "antisense" oligonucleotide approaches based upon the DNA sequence described herein, or by dominant negative mutant fucosyltransferase sequences that inactivate endogenous GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase(s) and that may be derived via mutagenesis and genetic selection schemes, in conjunction with the sequence information in this Disclosure.

This method could be used to construct animal cell lines that will be suitable host cells for the production of diagnostic or therapeutic materials whose usefulness or efficacy depends upon the specific post-translational modification determined by this cloned DNA sequence and its cognate enzyme. For example, it is known that that the biological effectiveness of many therapeutic proteins or peptides, recombinant or otherwise, may depend critically upon the oligosaccharide structure(s) that are covalently attached to them. The structure of these oligosaccharides is primarily a function of the number and kind of glycosyltransferase enzymes that are found in the cell used to produce these therapeutic products. Animal cells and yeasts are competent to perform these glycosylation reactions; however, not all glycosyltransferase enzymes are produced by every animal cell or yeast, and therefore, some oligosaccharide structures (including sub-terminal α(1,3) fucose residues generated by the enzyme encoded by the DNA sequence described here) are not produced by them. The converse is also true, namely, that producing cells may express a glycosyltransferase analogous to, or identical to, the GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase encoded by the DNA sequence described here. It is likely that sub-terminal α(1,3) fucose residues may alter the bioactivity (for better or for worse) of natural or recombinant therapeutic or diagnostic agents (glycoproteins or glycolipids) produced by mammalian or other eukaryotic hosts. Eukaryotic host cells that the biotechnology industry uses to produce tthese recombinant agents may be altered with the DNA sequence information and related information described in this invention, to add sub-terminal α(1,3) fucose residues to the oligosaccharides on recombinant product by expressing all or part of the cloned sequences described here in the desired host. Alternatively, sub-terminal α(1,3) fucose residues may be eliminated from the product produced in these host cells by the use of transfected "anti-sense" vector constructs, recombination-based gene inactivation, `anti-sense` oligonucleotide approaches, or dominant negative mutant fucosyltransferases, outlined above.

The old `methods` used for this process include an empirical approach to identify a cell line that does or does not express this particular enzyme or an enzyme that functions in a similar or identical manner, for the production of the appropriately modified recombinant or natural product. This is not always optimal since cell lines with this particular post-translation modification capabilities may not exist naturally, or may not be especially suited to high level production of an appropriately modified product. Alternatively, unwanted sub-terminal α(1,3)fucose residues present on a therapeutic material produced by an empirically identified animal cell line must be removed chemically or enzymatically, a process that may be costly or inefficient. The advantages of using the cloned, functional DNA sequence described here in conjunction with the technologies outlined above, relative to these older methods, include the ability to construct lines that specifically lack the capability to generate sub-terminal α(1,3)fucose residues on the oligosaccharides of glycoproteins and glycolipids; properly constructed, these cell lines will eliminate any need for chemical or enzymatic treatment of a therapeutic or diagnostic material to remove unwanted sub-terminal α(1,3)fucose residues. Moreover, in the event that sub-terminal α(1,3)fucose residues residues are found to be desirable for a particular diagnostic or therapeutic product produced by animal cells, cell lines may be engineered with the cloned DNA sequence described here to generate these residues.

ii. Isolation of reagents suitable for efficient enzymatic synthesis and production of oligosaccharides (in enzyme reactors, for example).

Oligosaccharides may have therapeutic utility as immunomodulatory reagents in the field of organ transplantation. In particular, soluble and solid-phase oligosaccharides may find use as therapeutic agents with which to block or ameliorate antibody-mediated organ transplant rejection in cases involving incompatibility due to differences in the major blood group antigen systems of the organ donor and the recipient, including the Lews blood group system. Likewise, soluble oligosaccharides may find use as therapeutic agents that function by blocking attachment of bacterial, viral, or parasitic pathogens to glycoconjugate "receptors" found on the surface of the animal tissues that these pathogens invade. For example there is evidence that portions of the Lewis blood group oligosaccharide antigen (containing sub-terminal α(1,3)fucose residues) serve as "receptors" for some forms of uropathogenic bacteria. Moreover, glycoconjugates, including sub-terminal α(1,3)fucose residues, have been implicated in modulating adhesive events between cells, and between cells and their environment during developmental and differentiation processes. These events included binding of spermatozoa to eggs, and the initial events that mediate attachment of fertilized ova to the uterine wall at the beginning of implanatation. These observations suggest, for example, the possibility that contraceptive uses for (biologically "natural") oligosaccharide molecules might exist. In addition, specific glycoconjugates containing sub-terminal α(1,3)fucose residues have been implicated as ligands for the LECCAM/Selectin family of adhesion molecules, that play important roles in mediating adhesion between cells of the immune system, and some tumor cells, and the surfaces of the endothelial cells that line the vascular tree. Thus, the cloned fucosyltransferase sequence described here may be used to construct oligosaccharide-type molecules, with pharmaceutical properties possessing anti-inflammatory and anti-tumor metastatic functions.

Currently, oligosaccharides containing sub-terminal α(1,3) fucose residues are produced by chemical synthesis (a procedure that is inefficient and costly) or by isolation from natural sources (using costly and inefficient procedures that often require the processing of large quantities of animal or plant material, and the purification of the desired oligosaccharide from other contaminating oligosaccharides). The invention described here provides a mechanism to synthesize abundant quantities of purified GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase. This could be used to construct an enzyme bioreactor (enzyme in solution or immobilized on a solid phase matrix, for example via the protein-A moiety fused to the catalytic domain of the enzyme, as described in previous manuscripts published by the Inventor John Lowe) capable of enzymatic synthesis of structures containing sub-terminal α(1,3) fucose residues. This may be more efficient than approaches involving chemical synthesis of structures containing sub-terminal α(1,3) fucose residues or their purification from natural sources, for a variety of reasons. One, the only chemicals necessary would be the enzyme substrates; these are easily obtained or synthesized. Two, enzymatic synthesis of such structures will produce only the desired product and the nucleotide diphosphate product of substrate hydrolysis. This latter chemical is found as the natural byproducts of these reactions in animal cells, is relatively non-toxic, and may be easily separated from the oligosaccharide synthetic product. By contrast, chemical synthetic procedures typically generate numerous products of side reactions which must be removed, and which may be toxic as well. Similarly, purification of oligosaccharides from natural sources requires the removal of other contaminating oligosaccharides present in the natural material. Three, enzymatic catalysis is extraordinarily efficient; essentially complete conversion of substrate to product might be achieved. By contrast, chemical synthesis of sub-terminal α(1,3) fucose residues on oligosaccharides is a multi-step process; yields at each step may be much less than 100%, and the cumulative efficiency of current chemical synthesis procedures does not approach the efficiency possible with enzymatic synthesis. Similarly, purification of oligosaccharides with subterminal α(1,3) fucose residues from natural materials can entail significant losses inherent to the purification procedures required to separate the desired oligosaccharide from contaminating, irrelevant oligosaccharides, with inefficient isolation of the desired oligosaccharide. Although the GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase encoded by the DNA sequence described here may be partially purified from animal tissues for synthetic use, these purifications are themselves inefficient, primarily because the enzyme is typically present in very low abundance. This invention provides two mechanisms that may provide for the abundant production of this enzyme. First, this may be done through the construction and selection of animal cells that produce relatively large quantities of the enzymes. Alternatively, this cloned nucleic acid sequence may then be used with standard recombinant DNA technologies to produce large quantities of glycosyltransferases in yeasts or in prokaryotic hosts. Furthermore, the sequence encoding this enzyme may be modified via standard molecular cloning schemes or mutagenesis to yield a recombinant fucosyltransferase with novel properties that make it more desireable than the wild-type enzyme. For example, the modifications might be made to the enzyme that make it more stable, or more suitable for immobilization in a bioreactor.

iii. Isolation of reagents suitable for producing recombinant GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase to be used directly as a research reagent, or to be used to generate antibodies against the GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase, for research applications.

This invention provides two mechanisms for producing large quantities of this enzyme (see ii. above--i.e. specially constructed animal cells, or via natural or synthetic genes encoding these enzymes) which may be used as a research tool with which to study the structures and functions of oligosaccharides and glycoproteins. Likewise, the enzyme produced by this method, or the nucleic acid sequence and derived protein sequence provided by this method, may be used to generate antibodies to this enzyme (via synthetic peptides). These antibodies might also be used as research reagents to study the biosynthesis and processing of these enzymes, and might be used as an aid in their purification for all the uses described in this disclosure.

iv. Antibodies to glycosyltransferases as diagnostic reagents.

Aberrant expression of GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase has been associated with malignancy in humans, suggesting that this enzyme might serve as a tumor marker for early detection of malignancy involving a number of human tissues. Enzyme tumor markers have typically been assayed in body fluids by activity assays, which may be subject to non-specificity due to competing glycosyltransferase activity. These assays may also be insensitive since it is possible that inactive enzymes might be useful as tumor markers but would not be detected by enzyme activity assays. This invention provides a mechanism for generating antibodies to this enzyme (monoclonal and polyclonal antibodies against synthetic peptides constructed from information derived from cloned DNA sequence encoding GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase, or against the recombinant enzyme produced by eukaryotic or prokaryotic hosts). Antibodies specific for this GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase so produced could be used to detect and quantitate this glycosyltransferase in body fluids, with specificity and sensitivity exceeding enzyme activity assays, and with the possibility of serving as a tumor marker for early detection of malignancy.

v. Recombinant enzyme for use in screening natural and synthetic compounds for fucosyltransferase inhibitors or inactivators.

A number of studies have noted an association between increased numbers of cell surface sub-terminal α(1,3) fucose residues on oligosaccharides of a cell and the ability of that cell to metastasize in a malignant fashion. If there is a causal relationship here, then it may be possible that drugs that inhibit the enzyme encoded by the sequence in this disclosure might be active as anti-tumor agents. Likewise, numerous recent studies have implicated sialylated and neutral oligosaccharides containing subterminal α(1,3) and α(1,4) fucose linkages in mediating adhesion of leukocytes to the selectin adhesion molecules (ELAM-1; GMP-140; Mel14/LAM-1) during inflammation. These studies suggest that molecules capable of preventing synthesis of α(1,3) and α(1,4) fucose linkages on leukocytes may thus function to diminish or even eliminate the ability of leukocytes to synthesize and display subterminal α(1,3) and α(1,4) fucose linkages, and would thus represent anti-inflammatory pharmaceutical agents. The reagents described in this disclosure may prove useful for screening to isolate or identify compounds that exhibit anti-fucosyltransferase activity, since the cloned sequence may be used with standard techniques to produce relatively large amounts of pure fucosyltransferase. This will aid in screening since the effects of potential inhibitors will be tested on a pure enzyme, without the confounding effects that may occur in whole cell extracts or with partially purified enzyme.

vi. Engineering of glycosyltransferase substrate specificity to generate novel glycoconjugate structures on secreted or cell-associated glycoconjugates.

This invention provides a reagent (a cloned GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferase gene segment), that, when used with appropriate mutagenesis and genetic selection schemes, may allow the generation of mutant GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferases that generate glycosidic linkages different from that generated by the wild-type enzyme. These novel linkages may or may not be naturally occuring, and could find utility as moieties that enhance the bioactivity of the molecules to which they are attached. Directed mutagenesis procedure may also be considered since this enzyme maintains primary sequence similarity to other α(1,3)-Fucosyltransferases, yet exhibits a distinct set of acceptor substrate utilization properties. Alternatively, mutagenesis and selection approaches may be used to generate mutant GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferases that act in a dominant negative fashion. The dominant negative mutants so generated might be used to inactivate endogenous glycosyltransferase activities when the product(s) of such an enzyme are not desired. Mutant GDP-Fuc: β-D-Gal(1,4)!-D-GlcNAc α(1,3)-Fucosyltransferases might also be generated, for example, that function as fucosidases that hydrolyze various sugar linkages (fucose, mannose, or others) from oligosaccharides in vitro and in vivo.

vii. Genotyping individuals at this fucosyltransferase locus.

Absence of a fucosyltransferase similar or identical to the one encoded by the DNA sequence detailed here has been found in several families. Should such absence be associated with a detrimental phenotype, DNA sequence polymorphisms within or linked to the gene corresponding to this cloned gene segment may be used to genotype individuals at this locus, for the purpose of genetic counseling. Likewise, the molecular basis for any such detrimental phenotypes might be elucidated via the study of the gene segment described here, should it be causally-related to such phenotypes.

Other features of this invention will become apparent in the course of the following description of exemplary embodiments which are given for illustration of the invention and are not intended to be limiting thereof.

EXAMPLES Example I. Cloning and Expression of a DNA Sequence Encoding α(1,2)Fucosyltransferase (DNA SEQ ID NO:5, protein SEQ ID NO:6

Mouse L Cells as a Host for Human (α-1,2)Fucosyltransferase Gene Transfer--Mouse L cells were tested as a host for gene transfer. These cells have been widely used for this purpose. Genomic DNA may be introduced into L cells with high efficiency, and these cells allow the use of several metabolic and antibiotic resistance schemes for selecting stable incorporation of exogenous DNA sequences.

L cells were examined for surface expression of the H Fuc α(1,2)Gal linkage, using a monoclonal antibody that recognizes type II H structures and fluorescence-activated cell sorting. Cells stained with this anti-H antibody exhibit a FACS profile virtually identical to the profile generated by cells stained with a control mouse IgM monoclonal antibody, and to profiles generated by cells stained only with FITC-conjugated second antibody. These results indicate that L cells do not express surface-localized Fuc α(1,2)Gal linkages that are detectable with the anti-H-antibody.

The inventor assayed L cell extracts to confirm that this absence of surface-expressed H determinants was due to a deficiency of (α-1,2)fucosyltransferase activity. Phenyl-β-D-galactoside was used as the acceptor for assay of (α-1,2)fucosyltransferase. This compound is a specific acceptor for (α-1,2)fucosyltransferases; it is used efficiently by these enzymes, yet does not function as an acceptor for fucosyltransferases that generate (α-1,3), (α-1,4), or (α-1,6) linkages. L cell extracts contained no detectable (α-1,2)fucosyltransferase activity, even in assays that contained increased amounts of extract, or that were subjected to prolonged incubation. Mixing experiments with A431 cell extracts showed that inhibitors were not responsible for an apparent lack of detectable enzyme activity.

The inventor also examined L cells for surface expression of glyconjugates possessing N-acetyllactosamine (Galβ(1,4)GlcNAc) end groups. These would represent potential acceptor molecules for a complementing human (α-1,2)fucosyltransferase activity and would allow the resulting surface-expressed Fucα(1,2)Gal linkages to be detected with anti-H antibody. The agglutinin from Erythrina cristagalli (ECA) was used for this analysis. This lectin exhibits high affinity for oligosaccharides possessing one or more unsubstituted N-acetyllactosamine end groups. L cells were stained with purified, FITC-labeled ECA, or with FITC-labeled ECA that had been preincubated with the hapten N-acetyllactosamine and were subjected to FACS analysis. The results indicated that significant amounts of ECA bind to these cells, and that the binding is effectively inhibited by the hapten N-acetyllactosamine. These results are consistent with the expectation that L cells synthesize oligosaccharides containing N-acetyllactosamine moieties and suggest that some of these glycoconjugates remain unmodified and are expressed at the cell surface.

The inventor also tested L cells for the ability to synthesize the fucosyltransferase substrate GDP-fucose. These analyses identified both GDP- ³ H!fucose and GDP- ³ H!mannose in aqueous extracts prepared from cells labeled with 2-³ H!mannose. The subcellular location of GDP- ³ H!fucose in these cells cannot be determined from these experiments. Effective Golgi fucosyltransferase activity would presumably require the presence of substrate concentrations of GDP-fucose within the lumen of the Golgi. Since these cells have not been selected to the defective in fucose metabolism, it seemed likely that they would be competent to transport this cytoplasmically synthesized compound into the Golgi lumen. This was confirmed by demonstrating that these cells are able to incorporate radio-labeled fucose into membrane glycoconjugates; most of this may represent fucose in α(1,6) linkage to the asparagine-linked N-acetylglucosamine of some N-linked oligosaccharides.

Taken together, these studies show that L cells are competent to display surface-localized H Fucα(1,2)Gal linkages, after introduction and expression of human DNA sequences determining (α-1,2)fucosyltransferase activity.

Human A431 Cells as a Donor for (α-1,2)Fucosyltransferase DNA Sequences--The human A431 cell line was investigated as a source of DNA for gene transfer since these cells express type I and II blood group H structures. Extracts prepared from A431 cells are found to contain (α-1,2)fucosyltransferase activity when assayed using phenyl-β-D-galactoside. The radiolabeled product elaborated by A431 extracts cochromatographed with authentic ¹⁴ C! fucosylated phenyl-β-D-galactoside produced by human serum H (α-1,2)fucosyltransferase. Digestion of the A431 product with α-L-fucosidase generated L-fucose in quantitative yield. These results indicate that A431 cells contain one or more functional (α-1,2)fucosyltransferase genes and thus represent an appropriate source of human DNA for gene transfer.

Isolation of a Mouse Transfectant That Expresses Surface Molecules Recognized by a Monoclonal Anti-H Antibody--To isolate mouse cells containing DNA sequences that determine expression of a human (α-1,2)fucosyltransferase, monolayer cultures of L cells were cotransfected with 30:1 ratio of high molecular weight genomic DNA prepared from A431 cells and pSV2-neo plasmid DNA. Cotransfection with pSV2-neo followed by growth of the transfected cells in media containing G418 allows selection of transfectants that have stably incorporated exogenous DNA sequences. With this procedure the inventor generated a population of cells representing approximately 60,000 independent G418-resistant transfectants. This method typically incorporates approximately 1000 kb of transfected sequences into the genome of a recipient cell. Since the size of the human genome is approximately 3×10⁶ kb, the inventor estimated that approximately 20 copies of the haploid human genome were represented within this "library" of primary transfectants.

Transfectants were selected for H antigen expression by a combination of panning and sterile cell sorting. A pool of cells representing the entire population of transfected cells was reacted with a mouse IgM monoclonal antibody that recognizes type II H structures. Transfectants with bound anti-H antibody were subjected to an initial selection by panning on sterile dishes coated with goat anti-mouse IgM. At this stage, the inventor found this procedure to be more effective than selection by flow cytometry because it allowed larger numbers of transfectants to be rapidly and easily processed. Transfectants selected by panning were returned to culture to be amplified for subsequent rounds of selection. The FACS profile of cells present after this first selection revealed no obvious peak of cells that had bound the anti-H antibody. However, analysis of the FACS histogram indicated that approximately 0.13% of the cells stained more brightly than cells stained with the control antibody. Cells representing the brightest 3-5% of the total population were aseptically collected, returned to culture for 14 days, and then reselected by the same procedure. After three selections, FACS analysis revealed the presence of a distinct population of cells that were brightly stained with the anti-H antibody. These cells were collected and returned to culture. For heuristic reasons, transfectants were also subjected to selection by panning, in parallel with the FACS selections. The inventor found that the panning procedure more efficiently enriched for populations of cells that bound the anti-H antibody. This is perhaps because the IgM anti-H antibody induced agglutination of H-positive transfectants and interfered with selection by FACS.

Therefore, all subsequent selections were performed by the panning procedure. After three additional rounds of panning (representing a total of seven rounds of selection), more than 90% of the cells within the selected population stained brightly with anti-H antibody. Clonal isolates from this population were generated, and individual subclones were analyzed for H antigen expression by FACS. Most clones gave rise to phenotypically mixed populations of cells consisting of H-expressing and non-expressing transfectants. The reasons for this apparent phenotypic instability are not known. One clone that exhibited a stable, bright H antigen-positive phenotype was selected for further analysis (clone mH1-12). The phenotype of clone mH1-12 has remained stable for more than 9 months in the absence of selection of H expression.

The inventor wished to rule out the possibility that a murine (α-1,2)fucosyltransferase gene might be active in rare variants in the L cell population or that the transfection procedure itself might activate this gene and that the selection process might enrich for these undesired events. Therefore, in a parallel control experiment, L cells were transfected with high molecular weight genomic DNA prepared from L cells, using pSV2-neo as the selectable marker. These transfectants were then subjected to selection for H antigen expression, exactly as described above. The inventor was unable to detect or isolate H-expressing cells from a population of independent transfectants (at least 40,000) that together has integrated the equivalent of more than 15 copies of the haploid murine genome.

The Primary Transfectant Expresses Cell Surface Type II Blood Group H Antigen and (α-1,2)Fucosyltransferase Activity--Clone mH1-12 was selected with a monoclonal anti-H antibody that recognizes type II blood group H structures (Fucα(1,2)Galβ(1,4)GlcNAc-R). Binding of this antibody to mH1-12 cells is blocked when the antibody is preincubated with the type II H hapten 2'-fucosyllactose (Fucα(1,2)Galβ(1,4)Glc). By contrast, preincubation of the anti-H antibody with L-fucose, or with N-acetyllactosamine or lactose, at identical concentrations, does not inhibit binding of the antibody to mH1-12 cells. When a different monoclonal anti-H antibody (BE2) previously shown to be specific for type II H structures was used in these experiments, the inventor also observed inhibition of binding with 2'-fucosyllactose, but not with the other haptens. These studies indicate that mH1-12 cells express cell surface glycoconjugates with terminal Fucα(1,2)Gal linkages.

Additional evidence for the presence of this linkage was obtained by using the linkage-specific blood group A (α-1,3)GalNAc transferase purified from human plasma. This glycosyltransferase has an absolute requirement for blood group H acceptors containing fucosyl α(1,2)galactoside as the terminal nonreducing group. It catalyzes the addition of N-acetylgalactosamine in α-1,3 linkage to the galactose moiety of this structure to construct blood group A-reactive molecules of the form GalNAα(1,3) Fucα(1,2)!Gal. Generation of blood group A-reactive determinants on the surface of mH1-12 cells by the action of blood group A glycosyltransferase would provide confirmation of the presence of terminal Fucα(1,2)Gal linkages inferred by the results of the type II H hapten inhibition study.

Formalin-fixed mH1-12 cells were incubated with preparations of the blood group A (α-1,3)GalNAc transferase and its nucleotide sugar substrate (UDPGalNAc, 1 mM, approximately 20-fold above K_(m) for UDP-GalNAc) in a buffer supporting activity of this enzyme. The cells were then probed for the presence of newly synthesized, surface-localized blood group A determinants by indirect immunofluorescence using a monoclonal anti-A antibody.

After a 4-h incubation with the A (α-1,3)GalNAc transferase and its substrate, blood group A determinants were detectable on the surface of the cells. No staining with anti-A antibody was observed in control reactions done in the absence of either UDP-GalNAc or group A transferase. L cells showed no binding of anti-H or anti-A under any of these conditions.

The inventor also stained A enzyme-treated cells with anti-H antibody to test for loss of surface expressed H structures. These should be "masked" with N-acetylgalactosamine molecules that are attached by the A enzyme to the galactose of the Fucα(1,2)Gal linkage. After a 4-h incubation with both A enzyme and its substrate, the staining generated by the anti-H antibody was only slightly diminished. However, after the conversion reaction was allowed to proceed for 24 h, essentially complete elimination of cell surface H reactivity was seen. This is coincident with continued expression of strong A reactivity. Cells treated for 24 h with a control reaction mixture containing the A enzyme but without substrate exhibited strong anti-H staining. This indicates that loss of H reactivity seen after the 24-h reaction was not due to destruction of H structures by glycohydrolase or protease activities contaminating the group A enzyme preparation. Loss of H reactivity after prolonged incubation thus represents "masking" of H structures by the A enzyme-catalyzed attachment of α-1,3-linked N-acetylgalactosamine. These data indicate that mH1-12 cells express cell surface glycoconjugates terminating with authentic H Fucα(1,2)Gal linkages.

Assays of extracts prepared from mH1-12 cells confirmed that these cells express α-(1,2)fucosyltransferase activity. α-Fucosidase digestion of the fucosylated product of these reactions confirmed the α anomeric linkage of the attached fucose.

Analysis of the Human DNA Sequences in the Primary Transfectant--Southern blot analysis was used to determine if the mH1-12 cell line contains human DNA sequences. The BLUR8 Alu sequence was used to detect human sequences. With the hybridization and washing conditions used, the human Alu probe did not cross-hybridize with mouse sequences, but was able to detect the equivalent of a few copies of an Alu sequence that had been added to mouse L cell DNA (10 μg). By comparison, the A431 DNA sample (3 ng) displayed a diffuse yet relatively intense hybridization signal expected for the highly repetitive interspersed Alu sequences. Under these conditions, the inventor was able to detect significant amounts of human sequences in the genome of mH1-12 cells (500 ng). This analysis indicates that, as expected, the genome of mH1-12 cells contains roughly 1000 kb of human DNA.

Isolation of Multiple Secondary Transfectants That Express Cell Surface Type II H Antigen and (α-1,2)Fucosyltransferase Activity--The inventor wished to be able to identify within the large number of human sequences in the genome of mH1-12 cells specific human sequences that mediate expression of its H-positive phenotype. To reduce the amount of extraneous human DNA, the inventor used DNA prepared from the mH1-12 cell line to generate "secondary" transfectant "libraries," and screened these libraries for transfectants that expressed the H structure. The inventor expected that H-expressing secondary transfectants so identified would each have a small number of human DNA restriction fragments identifiable within their genomes. The inventor sought to isolate several independent secondary transfectants since it was anticipated that human sequences linked to H-determining gene(s) should be a subset of these human fragments, identifiable as characteristic restriction fragments of identical sizes in each independently derived H-expressing secondary transfectant.

Genomic DNA prepared from mH1-12 cells was contransfected with pSV2-neo into L cells. Four different secondary libraries were generated in this way (Table 3).

                  TABLE 3     ______________________________________     Estimated frequencies of H antigen-positive     transfectants in six independent libraries     ______________________________________     Frequencies are expressed as one independent H-expressing     transfectant isolated/number of plates of transfectants     screened. For libraries screened by cell sorting or panning,     this is a minimum estimate since these immunoselection     procedures do not allow discrimination between H-expressing     sibs and independently derived H-positive transfectants; at     least one independent H-expressing transfectant was present in     each of these libraries. For the primary library mH1, and     secondary libraries mHs1, mHs3, mHs4, and mHs5, each plate     contained approximately 2000 independent transfectants, as     determined from transfection efficiency estimates (see     "Experimental Procedures"). For the mHs2 secondary library,     inspection of the plates prior to screening indicated that     approximately 50 colonies were present on each plate. Clones     s2-1 and s2-2 were isolated with the rosette procedure from     two separate plates. Clone s2-3 was isolated by panning a     population of cells representing approximately 650 independent     transfectant colonies.                                      Fraction of                 Source of  Added     transfectants                 transfected                            selection expressing H     Library name                 DNA        plasmid   determinants     ______________________________________     mH1 (primary)                 A431 Cells pSV2-neo  ≧1/30 dishes     mHs1 (secondary)                 mH1-12     pSV2-neo  ≧1/30 dishes     mHs3 (secondary)                 mH1-12     pSV2-neo  ≧1/10 dishes     mHs4 (secondary)                 mH1-12     pSV2-neo  ≧1/10 dishes     mHs5 (secondary)                 mH1-12     pSV2-neo  ≧1/10 dishes     mHs2 (secondary)                 mH1-12     None      ≧1/.sup.- 650 colonies                                      ≧1/.sup.- 50 colonies                                      (rosette s2-1)                                      ≧1/.sup.- 50 colonies                                      (rosette s2-2)     ______________________________________

Each library was independently screened for H-expressing transfectants using the panning procedure. Prior to the third round of panning, FACS analysis indicated that each of these libraries contained H antigen-positive cells (1-60% of the cells bound anti-H antibody). Sequential selection by panning was continued until 50-90% of the cells exhibited the H-positive phenotype. Clonal cell lines (S1-11 and S3-6) were then established from populations derived from the mHsl and mHs2 libraries; more than 95% of the cells in these lines exhibited bright staining with anti-H antibody. Libraries mHs4 and mHs5 were not subjected to the cell cloning procedure but were instead subjected to additional selections by panning. After a total of 11 rounds of selection, approximately 95% (selected from mHs4 library) and 50% (selected from mHs5 library) of these cells exhibited H antigen expression.

The calcium phosphate transfection procedure the inventor used for constructing the mH1-12 cell line occasionally results in physical linkage of selectable plasmid sequences with the transfected genomic DNA sequences that determine the desired phenotype. Linkage of pSV2-neo sequences to human DNA sequences determining the H-positive phenotype in the mH1-12 primary transfectant would simplify identification of H-expressing secondary transfectants and would facilitate isolation of the relevant transfected sequences by molecular cloning procedures. As a test of such linkage, the inventor generated a secondary library, mHs2, by transfecting L cells with DNA prepared from the mH1-12 primary transfectant. The transfection was done without the addition of exogenous pSV2-neo DNA. This procedure yielded approximately 50 independent G418 transfectants on each of fifteen 10-cm dishes. This represents a 40-fold reduction in the number of G418-resistant colonies obtained, relative to the numbers generated when secondary libraries were generated with the addition of pSV2-neo DNA (˜2000 G418 resistant cells/dish).

This mHs2 library was initially screened with an in situ rosette procedure for rapid identification of transfectant colonies that bound anti-H antibody (see "Experimental Procedures" below). Culture dishes containing approximately 50 colonies each were screened with this method 16 days after transfection and prior to any other manipulations. A single rosette-positive colony was identified on two of the 15 dishes tested. These two independent H-positive colonies were isolated with cloning rings and an H-expressing cell line (s2-1 and s2-2) was established from each. An additional, independent, clonal H-expressing transfectant (s2-3) was isolated by harvesting the colonies on the other 13 dishes and subjecting these cells to selection by panning and then cell cloning.

Cotransfection of unlinked single-copy markers occurs at a frequency less than 1%. The frequencies of coexpression of G418 resistance and the H phenotype the inventor observed in the mHs2 library (Table 3) are consistent with the possibility that the two markers are linked in the primary transfectant. Alternatively, these frequencies could be explained by cotransfection of unlinked markers present in multiple copies in the primary transfectant. In any event, the frequencies of H-expressing transfectants observed in the primary and secondary libraries (Table 3) indicate that the H-positive phenotype expressed by these transfectants is determined by a single transfected locus.

The anti-H reactive surface molecules on a representative H-expressing secondary transfectant (clone s2-2) were shown to be authentic H Fucα(1,2)Gal linkages, using analyses identical to those used for the H antigen-positive primary transfectant mH1-12. Extracts prepared from s2-2 cells were found to contain (α-1,2)fucosyltransferase activity. (α-1,2)Fucosyltransferase activity was also found in extracts prepared from each of the other H-expressing secondary transfectants.

Independent H-Expressing Secondary Transfectants Have Common Restriction Fragments Containing Human DNA Sequences--The inventor anticipated that the genome of each H-positive secondary transfectant would contain a relatively small amount of human DNA and that this DNA would include human sequences controlling expression of the (α-1,2)fucosyltransferase found in each. In principle, one or more characteristic restriction fragment(s) generated by these sequences should be identifiable in every transfectant. Conversely, irrelevant human sequences should exhibit a random restriction pattern in the secondary transfectants. The inventor therefore isolated genomic DNA from each transfectant, digested these DNAs with various restriction enzymes, and subjected these digests to Southern blot analysis. Restriction fragments containing human DNA sequences were detected with the BLUR8 Alu probe. A number of DNA restriction fragments are present in each clonal secondary transfectant; the aggregate amount of human genomic DNA present in these cells is estimated to be between 25 and 55 kb. The genome of each clonal secondary transfectant contains a characteristic pair of human DNA EcoRI restriction fragments with sizes of 2.7 and 3.4 kb. These fragments are also evident in pools of cells selected by panning from libraries mHs4 and mHs5. Similar analyses indicate that the genome of each H-expressing secondary transfectant contains common 1.5 and 1.9 kb PstI fragments and a common 2.8-kb PvuII human DNA restriction fragment. These observations imply that DNA sequences within or linked to these characteristic human restriction fragments are associated with expression of the cell surface H Fucα(1,2)Gal linkages used to select these transfectants, and are thus implicated in the expression of the (α-1,2)fucosyltransferase found in these cells.

To further confirm that the common human DNA sequences in the transfectants direct (α1,2)fucosyltransferase expression in these cells, molecular cloning procedures were used to isolate these fragments and then test their function in a mammalian transient expression system. The two human DNA EcoRI fragments previously found to be associated with expression of the H phenotype in the H-expressing secondary transfectants were isolated from mini genomic libraries prepared in a lambda phage vector, using the secondary transfectant s2-2 (see "Experimental Procedures"). To determine if these fragments contained sufficient genetic information to direct synthesis of (a1,2)fucosyltransferase, these were first individually subcloned into the mammalian expression cosmid vector pWE15. This vector contains the SV40 origin of replication, enabling it to replicate efficiently as an episome in COS-1 cells. The resulting plasmids contained either the 3.4 kb EcoRI (plasmid pH3.4) or the 2.7 kb EcoRI fragment (plasmid pH2.7). These plasmids were then individually introduced into COS-1 cells by DEAE-dextran transfection (see "Experimental Procedures") and the transfected cells were subsequently assayed for (α1,2)fucosyltransferase activity. The inventor found no detectable (α1,2)fucosyltransferase activity in mock transfected COS-1 cells or in COS-1 cells transfect with pH2.7. However, COS-1 cells transfected with plasmid pH3.4 expressed significant amounts of (α1,2)fucosyltransferase activity. α-Fucosidase digestion of the fucosylated phenyl-β-D galactoside product generated by this extract confirmed the alpha anomeric configuration of the attached fucose (see "Experimental Procedures").

The pH-activity profile of this (α1,2)fucosyltransferase mirrors the profiles determined for the (α1,2)fucosyltransferases found in fractionated human serum, in A431 cells, and in the H-expressing mouse transfectants. Likewise, the apparent Michaelis constants exhibited by the recombinant enzyme expressed in COS-1 cells (GDP-fucose K_(m) =17.5 μM; phenyl-β-D-galactoside K_(m) =4.4 mM) are essentially the same as those determined by the inventor for the (α1,2)fucosyltransferases in fractionated human serum, and in each of the cell lines he analyzed. Considered together, these results are consistent with the proposal that human DNA sequences within the 3.4 kb EcoRI fragment encode an (α1,2)fucosyltransferase, and that these sequences encompass part or all of the human blood group H (α1,2)fucosyltransferase gene. The 3.4 kb EcoRI fragment in pH3.4, the 2.7 kb EcoRI fragment in pH2.7, and DNA sequence adjacent to the 3.4 kb fragment, were sequenced to provide SEQ ID NO:5.

To characterize the nature of these cloned human genomic DNA sequences, the inventor first isolated various restriction fragments from the insert in plasmid pH3.4 and tested these for their ability to identify transcripts in the H-expressing, stable transfectants, and in a human cell line (A431) that also expresses H determinants and a cognate (α1,2)fucosyltransferase. He found that a 1.2 kb HinfI restriction fragment from the insert in pH3.4 identifies a single, relatively non-abundant 3.6 kb transcript in A431 cells. This probe also detects transcripts in the H-expressing mouse L cell transfectants, but not in the non-transfected parental L cells.

A cloned cDNA that directs expression of cell surface H structures and an (α1,2)fucosyltransferase. The inventor used the 1.2 kb HinfI fragment and colony hybridization to isolate two hybridization positive cDNA clones from an A431 cell cDNA library. To test the cloned cDNAs for their ability to determine expression of surface-localized H antigen and a cognate (α1,2)fucosyltransferase activity, a plasmid was constructed (pCDM7-α(1,2)FT, see "Experimental Procedures") that consisted of the largest cDNA insert cloned into the mammalian expression vector pCDM7, in the sense orientation with respect to the vector's enhancer-promoter sequences. Flow cytometry analysis of COS-1 cells transfected with PCDM7-α(1,2)FT indicates that this cDNA determines expression of cell surface H molecules. Moreover, CoS-1 cells transfected with pCDM7-α-(1,2)FT, but not cells transfected with pCDM7, express substantial quantities of an (α1,2)fucosyltransferase activity. The inventer determined the apparent Michaelis constant exhibited by this (α1,2)fucosyltransferase for an artificial acceptor (phenyl-β-D-galactoside) that is specific for this enzyme and that can discriminate between the human H and Se (α1,2)fucosyltransferases. This apparent Km (2.4 mM) is nearly identical to the apparent Km the inventor (3.1 mM,) and others (4.6 mM, 6.4 mM, 1.4 mM) have determined for the blood group H (α1,2)fucosyltransferase. Moreover, this apparent K_(m) is also very similar to the one exhibited by the (α1,2)fucosyltransferase in extracts prepared from COS-1 cells transfected with pH3.4 (4.4 mM). This apparent Km is distinct from the one exhibited by an (α1,2)fucosyltransferase found in human milk enzyme (15.1 mM), that is though to represent the (α1,2)fucosyltransferase encoded by the Se locus. These data demonstrate that the cDNA in plasmid pCDM7-α(1,2)FT determines expression of an (α1,2)fucosyltransferase whose kinetic properties reflect those exhibited by the human H blood group (α1,2)fucosyltransferase.

The cDNA sequence predicts a Type II transmembrane glycoprotein. The cDNA insert in pCDM7-α(1,2)FT is 3373 bases pairs long. Its corresponding transcript is 3.6 kb in length, suggesting that this cDNA is virtually full-length. Two potential initiator codons are found within its first 175 nucleotides. Only the second of these, however, is embedded within a sequence contest associated with mammalian translation initiation. The methionine codon initiates a long open reading frame that predicts a protein of 365 amino acids (SEQ ID NO:6), with a calculated Mr of 41,249 Da. This open reading frame is colinear with the open reading frame found in the 3.4 kb EcoRI fragment in pH3.4. Hydropathy analysis of the predicted protein sequence indicates that it is a Type II transmembrane protein, as noted for several other cloned glycosyltransferases. This topology predicts an 8 residue NH₂ -terminal cytosolic domain, a 17 residue hydrophobic transmembrane domain flanked by basic amino acids, and a 340 amino acid COOH-terminal domain that is presumably Golgi-resident and catalytically functional. Two potential N-glycosylation sites are found in this latter domain suggesting that this sequence, like other glycosyltransferases, may exist as a glycoprotein. No significant similarities were found between this sequence and other sequences in protein or DNA databases (Protein Identification Resource, Release 21.0, and Genbank, Release 60.0), with the exception of a 642 bp sequence within the 3'-untranslated segment of the cDNA that is similar to the human Alu consensus sequence. Moreover, the inventor identified no significant sequence similarities between this cDNA sequence or its predicted protein sequence, and those of other cloned glycosyltransferase cDNAs.

The protein encoded by the cDNA is an (α1.2)fucosyltransferase. The results of the expression experiments present above, when considered together with the domain structure predicted by the cDNA sequence, are consistent with the presumption that it encodes an (α1,2)fucosyltransferase. Nonetheless, the inventor wished to directly confirm this, and thus exclude the possibility that it instead encodes a molecule that trans-determines this enzyme activity. The inventor therefore fused the putative catalytic domain of the predicted protein to a secreted form of the IgG-binding domain of Staph. aureus protein A (see Experimental Procedures) in the mammalian expression vector pPROTA, to yield the vector pPROTA-α(1,2)FT_(c). By analogy to similar constructs the inventor has prepared with other cloned glycosyltransferases (detailed infra), he expected that if the cDNA sequence actually encodes an (α1,2)fucosyltransferase, then plasmid pPROTA-α(1,2)FT_(c) would generate a secreted, soluble, and affinity purifiable (α1,2)fucosyltransferase. Indeed, conditioned media prepared from a plate of COS-1 cells transfected with pPROTA-α(1,2)FT_(c) contained a total of 5,790 units of (α1,2)fucosyltransferase activity, whereas a total of 1,485 units were found to be cell-associated. Moreover, virtually 100% of the released (α1,2)fucosyltransferase activity was specifically retained by IgG-Sepharose, and most could be recovered after exhaustive washing of this matrix. By contrast, the inventor found that most of the activity in COS-1 cells transfected with pCDM7-α(1,2)FT was cell-associated (3450 units), with only trace amounts of activity in the conditioned media prepared from these cells (˜80 units). Virtually none of this latter activity bound to either matrix. Extracts prepared from COS-1 cells transfected with vector pCDM7 or vector PPROTA did not contain any detectable cell-associated or released (α1,2)fucosyltransferase activity. These data demonstrate that the cDNA insert in pCDM7-α(1,2)FT encodes an (α1,2)fucosyltransferase, and that information sufficient to generate a catalytically active (α1,2)fucosyltransferase is encompassed with the 333 amino acids distal to the putative transmembrane segment.

Experimental Procedures for Example I, "Cloning and Expression of a DNA Sequence Encoding (α1,2)Fucosyltransferase"

The term "L cells" used throughout the text refers to the mouse Laprt⁻ tk⁻ cell line.

Lactose, N-acetyllactosamine, 2'-fucosyllactose (Fucα(1,2) Galβ(1,4)Glc), UDP-GalNAc, phenyl-β-D-galactoside, and Ficoll 400 were obtained from Sigma. L-Fucose was from Pfanstiehl Labs (Waukegan, Ill.). UDP 1-³ H!N-acetylgalactosamine (8.7 Ci/mmol) and D- U-¹⁴ C!mannose (239 mCi/mmol) were from DuPont-New England Nuclear. D- 2-³ H!mannose (16.3 Ci/mmol), L- 6-³ H!fucose (72 Ci/mmol), L- 1-¹⁴ C!fucose (58.7 mCi/mmol), GDP U-¹⁴ C!-β-L-fucose (268 mCi/mmol), and α-³² P dCTP (3000 Ci/mmol) were from Amersham Corp. Nonradioactive GDP-fucose was kindly provided by Dr. Eric Holmes (Seattle). FITC-ECA was obtained from E-Y Labs (San Mateo, Calif.). Plasmid pSV2-neo was obtained from Dr. David Chaplin (Washington University, St. Louis). Restriction enzymes (New England Biolabs or Boehringer Mannheim) were used according to the manufacturer's instructions.

Antisera

Monoclonal anti-H, anti-A, and anti-B antibodies (mouse IgM) were purchased from Chembiomed, Ltd. (Alberta, Canada). Monoclonal anti-H antibody BE2 was prepared from BE2 hybridoma cell culture supernatants (see below). Anti-mouse IgM and FITC-labeled antimouse IgM (both antigen-affinity purified, goat) were from Sigma.

Cell Lines and Culture

Mouse Laprt⁻ tk⁻ cells were obtained from Dr. David Chaplin. Human A431 cells were from Dr. Brian Whiteley and Dr. Luis Glaser (Washington University, St. Louis). BE2 cells were obtained from the American Type Culture Collection. Cells were grown in Dulbecco's modified Eagle's medium (GIBCO) supplemented with 10% fetal calf serum (Hyclone, Logan, Utah). Transfected cells were grown in media containing G418 (GIBCO) at 400 μg/ml (active drug).

Preparation of Genomic DNA

High molecular weight genomic DNA was prepared from cultured cells by standard methods. Samples of genomic DNA were electrophoresed through 0.3% agarose gels buffered in Tris-acetate-EDTA to confirm their integrity and to estimate the average size of the molecules in the preparations.

Transfections

The calcium phosphate precipitation method was used to transfect mouse L cells with human genomic DNA. Cells (5×10⁵ /100-mm dish) were incubated overnight with DNA precipitates 20-30 μg of genomic DNA and 1 μg of pSV2-neo). No exogenous pSV2-neo DNA was included in transfections that generated the mHs2 secondary library. The cells were fed fresh media the following day and were placed under G418 selection the next day. Transfection efficiencies were estimated by harvesting transfected cells 1 day after addition of DNA and plating duplicate serial dilutions of the cell suspensions. One set of dilutions was grown under G418 selection, and the other was grown in the absence of antibiotic to allow the derived transfection efficiencies to be corrected for plating efficiency. After 2 weeks of growth, colonies were counted after staining with 0.2% methylene blue in 50% methanol. Approximately 2000 independent transfectants were typically obtained by transfecting 5×10⁵ cells on a 100-mm dish.

Immunologic Selection of H-expressing Transfectants

Transfectants were removed from culture dishes by incubating them with PBS containing 3 mM EDTA. Detached cells were washed and resuspended in staining medium (10 mM Hepes, pH 7.4, 0.1% sodium azide, 2% fetal calf serum in Dulbecco's modified Eagle's medium). The cells were kept at 4° C. throughout the panning or cell sorting procedures. Cell cloning was done by plating cells at low density, allowing individual cells to form colonies, and isolating individual colonies with cloning cylinders.

Panning--Bacteriological culture dishes (Falcon 1007, 60 mm) were prepared for panning. Goat antimouse IgM was coupled to the dishes by incubating them overnight at 4° C. with 4 ml of antibody solution diluted to 10 μg/ml in 50 mM Tris, pH 9.5. The antibody solution was aspirated, and the dishes were washed twice with PBS. The dishes were then blocked by incubating them at room temperature for at least 1 h with PBS containing 1 mg/ml bovine serum albumin. Dishes were then used immediately or were stored indefinitely at 4° C. The dishes were washed three times with PBS prior to use.

Cells to be panned were resuspended at a concentration of 10⁷ /ml, in staining media containing anti-H antibody at 10 μg/ml. The cells were incubated for 30 min at 4° C., and unbound antibody was removed by pelleting the cells through 10 ml of PBS containing 1 mM EDTA, 0.1% sodium azide, and 2% Ficoll 400. After centrifugation, the supernatant was carefully aspirated, and the cells were resuspended at 10⁶ /ml, in staining media. Three-ml aliquots of this cell suspension were applied to 60-mm panning dishes coated with goat anti-mouse IgM. The dishes were then incubated for 1 h at 4° C., and were then rinsed 5 times with serum-free Dulbecco's modified Eagle's medium to remove nonadherent cells. Fresh, serum-replete media was added to the dishes, and they were returned to the tissue culture incubator. The next day, adherent cells were removed with trypsin-EDTA and were replated on standard tissue culture dishes. These cells were grown for 10-18 days prior to the subsequent selection.

Cell Sorting--Transfectants were prepared for FACS analysis by incubating them for 30 min at 4° C. with monoclonal IgM anti-H antibody (10 μg/ml in staining media) or with a control monoclonal IgM anti-B antibody (10 μg/ml) in staining media). The cells were then washed in ice-cold staining media, and incubated for 30 min at 4° C. in staining media containing fluorescein-conjugated goat antimouse IgM at 40 μg/ml. The cells were washed, resuspended in staining media, and subjected to analysis by the FACS (Coulter Electronics model Epics C). Samples were gated on forward and 90° light scatter to eliminate dead cells from analysis. H-expressing cells were collected aseptically into staining media and then returned to culture for 10-18 days before additional selections.

Rosette Procedure--A resetting method was used to identify colonies of transfectants that bound anti-H antibody. This was done on 100-mm dishes containing isolated colonies comprised of approximately 100-300 cells. Plates of colonies were first rinsed twice with PBS and were then incubated for 1 h at 4° C. with 4 ml of mouse IgM monoclonal anti-H antibody at 10 μg/ml in PBS, 2% fetal calf serum, 0.1% sodium azide. The plates were then rinsed three times with PBS, and were incubated for 30 min at 4° C. with 4 ml of human erythrocytes conjugated with goat anti-mouse IgM. (Goat anti-mouse IgM was coupled to human blood group O red cells with chromic chloride. After conjugation, the red cells were washed with PBS, diluted to a 0.2% v/v suspension in PBS, 2% fetal calf serum, 0.1% sodium azide, and were used immediately.) Afterwards, the suspension of erythrocytes was carefully aspirated, and the plates were gently rinsed with PBS and examined on a light box. Colonies that had bound anti-H antibody were macroscopically visible as "rosettes" consisting of red cells adherent to the colonies.

Purification of Blood Group A (α-1.3)GalNAc Transferase

Group A transferase was isolated from human blood group A plasma by affinity chromatography on Sepharose 4B (Sigma lot no. 104F0334). Column fractions containing the peak of enzyme activity were pooled, mouse serum albumin (Behring Diagnostics >98%) was added to a 1% concentration, and aliquots were stored at -80° C. until use. The activity of the final preparation was determined by a standard radiochemical method. One enzyme unit is defined as one nmol of GalNAc transferred to 2'-fucosyllactose acceptor/h.

Paper Chromatography

Descending paper chromatography was performed using Whatman No. 3 mm or Whatmann No. 1 in the following solvent systems: Solvent A, ethyl acetate/pyridine/water (10:4:3); Solvent B pyridine/water/ethyl acetate (10:11:5:36), upper phase. The dried chromatograms were cut into 1-cm strips and radiolabeled compounds were eluted with water. Radioactivity in an aliquot of each eluate was determined by scintillation counting.

Analysis of L Cell GDP-Fucose Content

To identify GDP-fucose in mouse L cells, cells (2.5×10⁶) were labeled for 3 days with 250 μCi of D- 2-³ H!mannose in 30 ml of complete media. Cells were harvested and extracted with 60% ethanol for 5 min in a boiling water bath. The aqueous extract containing nucleotide sugars was concentrated under vacuum and resuspended in a small volume of water. Unlabeled GDP-mannose (270 nmol) and GDP-fucose (130 nmol) were added as internal standards, as the mixture was subjected to gel filtration chromatography on a Sephadex G-25 column (0.9×42-cm) equilibrated in 50 mM ammonium acetate. The eluate was monitored at 268 nm; fractions containing GDP-fucose and GDP-mannose were pooled, concentrated under vacuum, and resuspended in 200 μl of water. This was subjected to fractionation by HPLC on a weak anion exchange column (AX300, 4 mm×24 cm, Pierce Chemical Co.). The sample was applied to the HPLC column equilibrated in 100 mM triethylamine acetate, pH 7.0, and was eluted with a linear gradient from 100 to 300 mM triethylamine acetate, pH 7.0 in 50 min at a flow rate of 2 ml/min. The eluant was monitored at 268 nm, and fractions (0.5 ml) were collected for scintillation counting and subsequent analysis. The unlabeled nucleotide sugar internal standards were identified by their characteristic elution times (GDP-fucose, 35.5 min. approximately 800 cpms; GDP-mannose, 33.0 min. approximately 1000 cpms). The radioactive peaks corresponding to the fractions coeluting with unlabeled GDP-fucose and GDP-mannose were subjected to hydrolysis with 0.1 N HCl for 45 min at 100° C. These were then fractionated by descending paper chromatography on Whatman No. 3MM in solvent B for 20 h, in parallel with L- ¹⁴ C!fucose and D- ¹⁴ C! mannose standards. In each case, approximately 30% of the counts hydrolyzed were recovered as the appropriate monosaccharide.

Analysis of Fucose-Labeled Glycopeptides

Radiolabeled glycopeptides were prepared and analyzed. Mouse L cells (2×10⁶) were labeled for 3 days with 200 μCi of L- 6-³ H!fucose in 20 ml of complete media. Cells were harvested and extracted with chloroform, and then water, and the pellet remaining after the final extraction was subjected to exhaustive digestion with Pronase (Behring Diagnostics). This was then desalted by gel filtration chromatography on Sephadex G-25-80. This material was concentrated under vacuum, and an aliquot was hydrolyzed in 0.1 N HCl at 100° C. for 45 min. The hydrolysate was then subjected to descending paper chromatography on Whatman No. 3MM in solvent B for 20 h, in parallel with an L- ¹⁴ C!fucose standard. Approximately 26% of the counts present in the macromolecular material were released and cochromatographed with the radiolabelled fucose standard.

Assay of GDP-L-Fucose:β-D-Galactoside 2-α-L-Fucosyltransferase

Cultured cells were washed with PBS, pelleted by centrifugation, and resuspended in a small volume of 25 mM sodium phosphate, pH 6.1, containing 0.5% Triton X-100. Volumes were adjusted to achieve a protein concentration of approximately 5 mg/ml (BCA method, Pierce Chemical Co.). Extracts were typically assayed immediately after preparation. The standard assay contained 5-20 μl of enzyme solution (typically 30-100 μg of cell extract protein or 15 μl of serum) in 40 μl of 25 mM potassium phosphate, 0.1% Triton X-100, 3 μM GDP- ¹⁴ C!fucose, 25 mM phenyl-β-D-galactoside, and 5 mM ATP. The pH of the reaction mixture was adjusted to a final measured pH of 6.1. Assays were terminated after an appropriate period of time by the addition of 20 μl of ethanol. The mixture was then centrifuged at 15,000×g for 5 min. The supernatant was collected, spotted on Whatman No. 1, and subjected to fractionation by descending paper chromatography for 4 h in Solvent A. Radioactivity was then determined as described above. In all cases, parallel reactions were done in the absence of added phenyl-β-D-galactoside acceptor to allow correction for endogenous acceptor molecules. No products of endogenous acceptor molecules cochromatographing with fucosylated phenyl-β-D-galactoside were identified in any samples.

α-Fucosidase Digestion

¹⁴ C!Fucosylphenyl-β-B-galactoside (approximately 10,000 cpm) was isolated from fucosyltransferase assays by paper chromatography, concentrated from the water eluate under vacuum, and resuspended in 5 μl of water. This was digested with 0.025 units of bovine kidney α-L-fucosidase (EC 3.2.1.51) in a final volume of 20 μl containing 5 mM sodium citrate, pH 6.0, at 37° C. for 1 h. This mixture was then fractionated by descending paper chromatography on Whatman No. 1 in Solvent A for 4 h. Products of the digestions were identified by comparison to parallel separations of authentic L- ¹⁴ C!fucose, and purified ¹⁴ C! fucosylphenyl-β-D-galactoside synthesized by human plasma H (α-1,2)fucosyltransferase.

Indirect Immunofluorescence

Immunofluoroscence was performed on cells plated on 8-well tissue culture chamber slides (Lab-Tek). Cells were plated at a density of 5×10⁴ /well 24 h prior to analysis. Anti-H and anti-A primary antibodies were diluted in PBS containing bovine serum albumin at 2 mg/ml, to a final concentration of 10 μg/ml. Cell-bound primary antibodies were detected with FITC-conjugated goat anti-mouse IgM, diluted to 40 μg/ml in PBS containing 2 mg/ml bovine serum albumin.

Hapten Inhibition--Plated cells were washed twice with PBS, and were incubated for 30 min at 4° C. with 100 μl of diluted anti-H antibody, or with 100 μl of diluted antibody containing different oligosaccharide haptens, each at a concentration of 20 mM. The chambers were then washed twice, and 100 μl of FITC-conjugated goat anti-mouse IgM was added. After 30 min at 4° C., the chambers were washed three times with PBS, and the cells were fixed at room temperature for 10 min in 3.7% formaldehyde in PBS. The cells were washed twice with PBS, the chambers were removed, and the slide was mounted in PBS containing 25% glycerol. Cells were examined by fluorescence microscopy using a Zeiss Axiophot photomicroscope equipped with fluorescence epiillumination.

Labeling of Intact Cells with Human Blood Group A (α-1,3)GalNac Transferase--Cells plated in culture slide chambers were washed twice with 150 mM NaCl and were then fixed for 10 min at room temperature in 3.7% formaldehyde in 150 mM NaCl. The cells were washed three times with 150 mM NaCl and were then incubated with 100 μl of complete transferase reaction mixture or a control mixture. The complete transferase mixture consisted of 150 mM NaCl, 15 mM MnCl₂, 50 mM sodium cacodylate, pH 6.8, 0.2% bovine serum albumin, 1 mM UDP-GalNAc, and 1.14 units of human blood group A (α-1,3)GalNAc transferase. Control mixtures consisted of identical components except for the omission of either UDP-GalNAc or blood group A (α-1,3)GalNAc transferase. Incubations were performed at 37° C. and were terminated after either 4 or 24 h by washing the chambers twice with PBS. The cells were then analyzed by indirect immunofluorescence using mouse monoclonal anti-A or anti-H antibodies, as described above for the hapten inhibition studies.

Southern Blotting

Genomic DNA was digested to completion with restriction enzymes (8 units/μg DNA, overnight digestion). The restriction fragments were fractionated by electrophoresis through 0.6% agarose gels buffered in Tris-borate-EDTA. The DNA fragments were then transferred to nylon membranes (Hybond-N, Amersham Corp.), according to the standard Southern blotting method. Blots were prehybridized at 39° C. for at least 2 h in 50% formamide, 5 SSC (1×SSC is 150 mM NaCl, 15 mM sodium citrate, pH 7.0, 1×PE is 50 mM Tris, pH 7.5, 0.1% sodium pyrophosphate, 1% sodium dodecyl sulfate, 0.2% polyvinylpyrrolidone (M_(r) 40,000), 0.2% Ficoll (M_(r) 400,000), and 5 mM EDTA), and 150 μg/ml denatured salmon sperm DNA. Hybridizations were done in the same solution at 39° C. for at least 16 h. Blots were washed four times at room temperature in 2×SSC, 0.1% sodium dodecyl sulfate, and then once for 30 min at 65° C. in 0.75×SSC, 0.5% sodium dodecyl sulfate. The BLUR8 probe consisted of a 300-base pair BamHI segment isolated from the BLUR8 plasmid. This fragment was subjected to two cycles of gel purification prior to labeling to ensure that it was free from contaminating plasmid sequences. Probes were labeled with α³² p!dCTP to a specific activity of at least 5×10⁸ cpm/μg using the random priming method.

Paper Chromatography

Descending paper chromatography was performed using Whatman No. 40 in ethyl acetate/pyridine/water (10:4:3; Solvent A) or using Whatman No. 3MM in 95% ethanol/1M ammonium acetate (7:3; Solvent B). ¹⁴ C-Labelled compounds were located by autoradiography of dried chromatograms. Alternatively, the dried chromatograms were cut into 1 cm strips and the radiolabelled compounds were eluted with water. An aliquot of each eluate was mixed with scintillation cocktail and radioactivity was determined in a scintillation counter.

Preparation of Radiolabelled Standards

¹⁴ C!Fucose-1-phosphate was prepared by enzymatic cleavage of GDP- ¹⁴ C!fucose (1 nmol) with snake venom phosphodiesterase (EC 3.1.4.1, 1 μl, 0.003 units, Boehringer-Mannheim) in 20 μl of 100 mM Tris-HCI, pH 8 at 37° C. for 1 h. The reaction was then fractionated by descending paper chromatography on Whatman No. 3MM using Solvent B for 20 h in parallel with GDP- ¹⁴ C!fucose and ¹⁴ C!fucose. ¹⁴ C Fucose-1-phosphate (R_(fucose) =0.45) was then eluted from the chromatogram with water and concentrated under vacuum. ¹⁴ C!Fucosylphenyl-β-D-galactoside was generated from GDP- ¹⁴ C!fucose (3 μM) and phenyl-β-D-galactoside (25 mM) by the action of (α1,2)fucosyltransferase activity in human serum, using the reaction conditions described below for assay of α(1,2)fucosyltransferase. The products of this reaction were fractionated by descending paper chromatography on Whatman No. 40 for 4 h in Solvent A. ¹⁴ C! Fucosylphenyl-β-D-galactoside was identified by autoradiography of the dried chromatogram and was then eluted from the paper with water and concentrated under vacuum. Alternatively, ¹⁴ C! fucosylphenyl-β-D-galactoside was isolated from fucosyltransferase assay mixtures using the Sep-Pak procedure described below.

Cell Lines and Cell Culture

COS-1 cells were obtained from the American Type Culture Collection and were grown in Dulbecco's modified Eagle's medium containing 10% fetal calf serum.

Protein Determinations

Protein concentrations were determined by the BCA method (Pierce Chemical Co.) according to the manufacturer's instructions. Bovine serum albumin was used as the standard.

Preparation of Cell Extracts

Cells were washed with PBS, removed from culture dishes with a rubber policeman, and pelleted by centrifugation. Cell pellets were resuspended in 2 volumes of cold 1% Triton X-100 (Surfactamps X-100, Pierce Chemical Co.) and sonicated for 15 seconds using a Branson sonicator equipped with a micro tip at 50% power. These extracts were either assayed immediately, or were stored at -20° C. until use. Under these conditions, enzyme activity in the frozen extracts was stable for several weeks, but deteriorated rapidly on repeated freezethawing. Tissues from C3H mice were isolated, minced with a razor blade, suspended in 2 volumes of 1% Triton X-100 and sonicated as described above, centrifuged at 1500×g for 5 min and the supernatants were collected. Mouse intestinal mucosa extracts were prepared by everting the small intestine onto a thin polypropylene rod, scraping the mucosa cells into phosphate buffered saline, and collecting the cells by centrifugation at 1500×g. Extracts were then prepared as described above.

Partial Purification of Mouse Intestinal (α1,2)Fucosyltransferase

Preliminary experiments indicated that extracts prepared from mouse intestinal mucosa contained large amounts of an activity that hydrolyzed GDP-fucose in an acceptor-independent manner. Since substrate hydrolysis interfered with accurate determination of (α1,2)fucosyltransferase activity, intestinal extracts were fractionated by anion exchange chromatography to separate GDP-fucose hydrolysis activity from (α1,2)fucosyltransferase activity. All procedures were performed at 40° C. Two ml of the Triton-solubilized extract was made 10 mM in Tris-HCl, pH 7.6, and treated for 5 min with 2 ml (bed volume) of DEAE-cellulose (DE52, Whatman) previously equilibrated with 10 mM Tris-HCl, pH 7.6. The enzyme solution was filtered, made 10 mM in sodium phosphate buffer and the pH was adjusted to 6.1. Approximately 47% of the enzyme activity present in the initial extracts was recovered after the DEAE-cellulose fractionation procedure. A second treatment with DEAE-cellulose resulted in substantial loss of enzyme activity with no significant reduction in GDP-fucose hydrolysis activity (data not shown). When used under standard fucosyltransferase assay conditions, but in the absence of added acceptor, this preparation hydrolyzed less than 2% of the GDP-fucose initially present in the reaction.

Ammonium Sulfate Fractionation of Serum

Serum was prepared from freshly drawn blood obtained from a non-Secretor individual. The blood was clotted in a glass tube at 37° C. for 1 h and was immediately fractionated by ammonium sulfate precipitation exactly as described. The 20%-40% ammonium sulfate fraction was dialyzed against 2 changes of 4 liters of water (8 h each) at 4° C. Assay of (α1,2)fucosyltransferase was done immediately. Alternatively, the fractionated serum was aliquoted and stored at -20° C. until use.

Ion Exchange Chromatography of Human Milk (α1,2)Fucosyltransferase

(α1,2)Fucosyltransferase was isolated from human milk by published procedures. Briefly, 300 ml of milk from a Se-positive donor was defatted by centrifugation and was extensively dialyzed against 20 mM sodium cacodylate, pH 6.0. This was applied to a column (2.6×115 cm) of sulfopropyl-Sephadex equilibrated in 20 mM cacodylate, pH 6.0. The column was then washed with 1 liter of 20 mM cacodylate, pH 6.0, and was eluted with a linear gradient made from 1.5 liter each of 20 mM cacodylate, pH 6.0, and 500 mM NaCl in 20 mM cacodylate, pH 6.0. Fractions (13 ml) were collected and assayed for (α1,2)fucosyltransferase activity using phenyl-β-D-galactoside acceptor as described below. Fractions 130-144 contained (α1,2)fucosyltransferase activity. These were combined, concentrated to 1 ml by ultrafiltration in an Amicon stirred cell fitted with a YM5 membrane (MW cutoff=5,000), and were then equilibrated against cold deionized water. The concentrated enzyme was aliquoted and stored at -80° C.

Synthesis and Characterization of GDP-β-L-fucose and GDP-α-L-fucose

GDP-β-L-fucose was synthesized and purified by modifications of the method of Nunez et al. (Nunez et al, Can. J. Chem., 59, 2086-2095, 1981). The modified method described here eliminates the need to separate the anomeric 1-fucopyranosyl phosphates by differential crystallization prior to subsequent synthetic steps. Separation of anomeric products is effected by HPLC subsequent to the last synthetic procedure.

Pyridine and tetrahydrofuran (Aldrich) used in the synthesis were boiled under reflux over calcium hydride, distilled and stored over 4 Å molecular sieves. All evaporations were performed under reduced pressure on a rotary evaporator, with a bath temperature below 35° C. L-Fucose was acetylated exactly as described in Nunez et al (Can. J. Chem., 59, 2086-2095, 1981). The resulting mixture of 2,3,4-tri-O-acetyl-α- and β-L-fucopyranoses (2.7 g, 9.27 mmol) was phosphorylated using o-phenylene phosphochloridate. The crude reaction product containing a mixture of anomeric 1-fucopyranosyl phosphates was fractionated on a Dowex-1 column (HCO₃ ⁻, 20-50 mesh, 1.5×23 cm) preequilibrated in water. After application of the anomeric mixture, the column was washed with water (500 ml) and eluted with 400 mM triethylammonium bicarbonate buffer, pH 7.5 (250 ml). The triethylammonium bicarbonate eluant was evaporated to a thick syrup under reduced pressure. The syrup was dissolved in water and evaporated to dryness. This partially purified anomeric mixture of 1-fucopyranosyl phosphates (bis-triethylammonium salt, crude yield 70%)_was used for the synthesis of GDP-β-L-fucose. The anomeric 1-fucopyranosyl phosphates (200 mg) were first repeatedly dissolved in dry pyridine and then evaporated to dryness in vacuo. Guanosine 5'-phosphomorpholidate (400 mg) was then added to the dried anomeric 1-fucopyranosyl phosphates. This mixture was subjected to repeated resuspension in dry pyridine and evaporation to dryness in vacuo. The reaction mixture was then suspended in dry pyridine (15 ml) and was incubated at room temperature. The formation of GDP-β-L-fucose was monitored daily by high performance liquid chromatography (HPLC) on a weak anion exchange column (AX 300, 4.6 mm×22 cm, Pierce Chemical Co.). An aliquot (10 μl) from the reaction mixture was evaporated to dryness, dissolved in water and mixed with GDP- ¹⁴ C!fucose (2000 cpm). The sample was then applied to the HPLC column equilibrated in water, and was eluted with a linear gradient from 100% water to 250 mM triethylammonium acetate, pH 7.0, in 60 min at a flow rate of 2 ml/min. The eluant was monitored at 268 nm, and 0.5 ml fractions were collected for scintillation counting to identify the co-injected GDP- ¹⁴ C!fucose. Three peaks absorbing at 268 nm eluted at approximately 180 mM triethylammonium acetate. The retention time of the first peak (38.5 min) was identical to the retention time of GDP-α-L-fucose (see below). The second peak eluted at 40.6 min and coeluted with the GDP-β-L- ¹⁴ C!fucose standard. A third small peak eluting at 42.4 min was not identified. The reaction was judged to be essentially complete after 5 days. The proportion of α to β anomer in the final reaction mixture was found to be approximately 2:3. The reaction was then evaporated to dryness and dissolved in water. GDP-β-L-fucose was purified from this solution on a preparative Hydropore AX column (21.4 mm×25 cm, Rainin Instruments Co.). Aliquots of the aqueous solution were applied to the column equilibrated in water, and the sample was eluted with a linear gradient of 100% water to 200 mM triethylammonium acetate, pH 7.0, in 45 min, at a flow rate of 10 ml/min. The eluant was monitored at 268 nm. GDP-α-L-fucose eluted at 36.2 min. GDP-β-L-fucose eluted at 38.5 min. The GDP-β-L-fucose peak was collected and evaporated to dryness under reduced pressure. Ammonium acetate was removed by repeated co-evaporation with water. The compound that co-eluted with GDP- ¹⁴ C!fucose, and that we tentatively identified as GDP-β-L-fucose, was subjected to negative ion fast atom bombardment mass spectrum analysis (xenon), using a VG mass spectrometer (model 7B-250S). The results (m/z M-H!⁻ 588) were consistent with this identification. The compound was then analyzed by proton decoupled ¹³ C NMR spectroscopy to confirm the anomeric configuration of the fucose, and to further establish the identity of the compound as GDP-β-L-fucose. Proton decoupled ¹³ C NMR spectra were obtained on a Bruker WM 360, operating at 909.5 MHz and a sweep width of 221 ppm with 32K data points. Probe temperature was 38±° C. Resonances are reported in ppm relative to tetramethylsilane. This analysis yielded the following spectral data: ¹³ C NMR (40 μM in D₂ O, pH 7.0); guanine: δ140.27 (C1 and C8), 156.46 (C2), 154.38 (C4), 118.85 (C5), 161.46 (C6); ribosyl moiety: 89.36 (C1), 76.06 (C2), 73.6 (C3), 86.4 (C4), 67.8 (C5); fucosyl moiety: 100.9 (C1), 75.09 (C2), 73.94 (C3), 73.71 (C4), 73.02 (C5), 17.35 (C6). The distinction between anomeric forms of GDP-L-fucose is based upon the chemical shift of anomeric C(1) of the fucose ring. The C(1) resonance of the fucose ring in the β-anomer is shifted downfield (100.9 ppm) relative to the resonance of the anomeric C(1) in the a anomer (98.31 ppm, see below). The values obtained for this compound are essentially identical to those reported by Nunez et al. for GDP-β-L-fucose. The resonances attributable to the C atoms of the guanine, ribose and fucose ring are also in agreement with the literature values.

GDP-α-L-fucose was synthesized in a manner similar to that described for the preparation of GDP-β-L-fucose, except that the dicyclohexylammonium salt of (α-L-(-)fucose-1-phosphate (Sigma) was used instead of the anomeric mixture of 1-fucopyranosyl phosphates. Analysis and purification of GDP-α-L-fucose was performed by HPLC using the same conditions described for GDP-β-L-fucose. The purified compound, tentatively identified as GDP-α-L-fucose, was subjected to analysis by negative ion fast atom bombardment mass spectroscopy. The results (m/z M-H!⁻ 588) were consistent with this assignment. The subsequent analysis of the compound by ¹³ C NMR spectroscopy yielded the following spectral data: ¹³ C NMR (50 μM in D₂ O, pH 6.97); guanine: δ140.27 (C1 & C8), 156.46 (C2), 154.41 (C4), 118.84 (C5), 161.46 (C6); ribosyl moiety: 89.35 (C1), 76.04 (C2), 71.98 (C3), 86.4 (C4), 67.8 (C5); fucosyl moiety: 98.31 (C1), 70.36 (C2), 72.99 (C3), 74.3 (C4), 70.36 (C5), 17.88 (C6). The C(1) resonance of the fucose ring in this compound (98.31 ppm) is consistent with an α anomeric configuration, as reported by Nunez et al. The resonances attributable to the C atoms of the guanine and the ribose ring are also in agreement with the literature values reported for these atoms in GDP-β-L-fucose. GDP-β-L-fucose was found to be inactive as a substrate for (α1,2)fucosyltransferase.

Assay of GDP-L-fucose:β-D-galactoside:2-α-L-fucosyltransferase

Fucosyltransferase assays were performed by a modification of the procedure reported by Chester et al. (Chester et al, Eur. J. Biochem., 69: 583-593, 1976). The standard assay contained GDP- ¹⁴ C!fucose (3 μM), phenyl-β-D-galactoside (25 mM), ATP (5 mM) and the enzyme solution (1-10 μl) in 20 μl of 25 mM sodium phosphate buffer, pH 6.1. Based upon preliminary assays, amounts of added enzyme activities were adjusted to ensure that reactions were linear throughout the period of incubation (4 h for fractionated serum, 2 h for each of the other enzyme preparations). Under these conditions, less than 15% of the substrate was consumed during the incubation period. For the determination of pH optima, assays were buffered with 25 mM sodium acetate, sodium phosphate, or Tris-HCl, using concentrated solutions of these buffers previously adjusted to various pH values. The final pH value of each reaction was determined with a micro pH probe. In assays to determine the apparent Km values for GDP-fucose, GDP- ¹⁴ C!fucose was diluted with unlabeled GDP-fucose to a final specific activity of 26.3 mCi/mmol. The concentration of GDP-fucose in this stock solution was calculated from the UV absorbance at 254 nm of an aliquot diluted in water. The molar extinction coefficient of GDP (ε=13800 at 254 nm, pH 7.0) was used for this calculation since the extinction coefficient of GDP-fucose is not known. This stock was then used to yield variable GDP-fucose concentrations (3-300 μM) in the assays. The concentration of phenyl-β-D-galactoside in these assays was 25 mM, for all but the milk-derived enzyme. This was assayed in the presence of 75 mM phenyl-β-D-galactoside since the apparent Km exhibited by this enzyme for this acceptor is 15.1 mM. In assays to determine apparent Km values for phenyl-β-D-galactoside, the concentration of the acceptor was varied from 0.5 to 100 mM. GDP- ¹⁴ C!fucose was present in these assays at a concentration of 3 μM. The relatively low specific activity of commercially available GDP- ¹⁴ C! fucose, and its cost, necessitated the use of a substrate concentration well below its K_(m) in these experiments. All assays were performed at 37° C. Assays were terminated after an appropriate period of time by addition of 20 μl of ethanol, followed by dilution with 1 ml of water. The mixture was then centrifuged at 15,000×g for 5 min. The supernatant contained virtually 100% of the radiolabelled product; this was collected, and an aliquot was used for separation and quantitation of the fucosylated product using the hydrophobic interaction chromatography method described below. All assays were performed in duplicate or triplicate and included parallel incubations done in the absence of acceptor. The values obtained in the absence of exogenous acceptor were subtracted to correct for the presence of endogenous acceptor molecules. This background acceptor "activity" was always less than 3% of the radioactivity incorporated into the phenyl-β-D-galactoside acceptor. Apparent Michaelis constants were derived from Lineweaver-Burke plots of acceptor concentration-rate determinations. Intercepts were calculated by the least squares estimation method.

A separation procedure based upon hydrophobic interaction chromatography was developed for rapid processing of large numbers of samples. This procedure effects separation of ¹⁴ C!fucosylphenyl-β-D-galactoside product from GDP- ¹⁴ C!fucose on disposable C-18 Sep-Pak cartridges (Waters-Millipore). The bottom of the Sep-Pak cartridge is mounted in one hole of a two-holed rubber stopper, and a 5 ml syringe is attached to the top of the cartridge. The other hole in the stopper is attached to a vacuum source (approximately 350 mm of Hg). The mounted Sep-Pak cartridge is prepared by washing with 5 ml of acetonitrile, followed by 5 ml of water. This is done by separately pipetting each wash solution into the attached syringe barrel and drawing it through the cartridge under the vacuum created when the rubber stopper (with attached vacuum line) is pressed onto the top of a 20 ml plastic scintillation vial. The contents of the syringe are aspirated through the cartridge into the vial. The fucosyltransferase reaction sample is then loaded into the syringe and aspirated through the cartridge into a fresh scintillation vial. The ¹⁴ C!fucosylphenyl-β-D-galactoside is retained on the Sep-Pak cartridge. The cartridge is then washed sequentially by aspiration with three 2 ml portions of water, collecting each wash into a new scintillation vial. The ¹⁴ C!fucosylphenyl-β-D-galactoside product is then eluted by aspiration with three 2 ml portions of 50% acetonitrile. Scintillation cocktail (10 ml, Biosafe II, Research Products International) is added to each vial to obtain a clear solution and the radioactivity in each is determined by scintillation counting. Radioactivity eluting in fractions 1-4 represents GDP- ¹⁴ C!fucose, fucose-1-phosphate, and ¹⁴ C!fucose present either as a contaminant in the substrate as obtained from the manufacturer (approximately 1%), or that is formed by substrate or product hydrolysis. Fractions 5-7 represent ¹⁴ C!fucosylphenyl-β-D-galactoside. The recovery of radioactivity with this procedure exceeds 97%. Reconstruction experiments showed that recovery of pure ¹⁴ C!fucosylphenyl-β-D-galactoside is essentially 100%. Separation and recovery are independent of pH and enzyme source, and are tolerant of detergents (Triton X-100 and Lubrol-PX) at concentrations up to 2%. Product separation by this method requires approximately one minute per sample. Sep-Pak cartridges have been reused indefinitely with no deterioration in performance.

The Sep-Pak method does not separate GDP- ¹⁴ C!fucose from ¹⁴ C!fucose-1-phosphate and thus cannot detect nucleotide pyrophosphatase activity that may consume GDP-fucose while generating fucose-1-phosphate and/or fucose. ATP (5 mM) was therefore included in all assays to inhibit nucleotide pyrophosphatase activity. Effective inhibition of pyrophosphatase activity was confirmed by descending paper chromatography analysis of mock fucosyltransferase assays using each enzyme preparation. Each enzyme source was incubated for 1 to 4 h at 37° C. in 20 μl of standard assay cocktail, but in the absence of phenyl-β-D-galactoside. After the reactions were terminated, an aliquot of each was spotted on Whatman No. 3MM, fractionated by descending paper chromatography for 20 h in solvent B, and radioactivity was determined as described above. Authentic GDP- ¹⁴ C!fucose, ¹⁴ C!fucose-1-phosphate, and L- ¹⁴ C!fucose standards were chromatographed in parallel. This system separates GDP-fucose, fucose-1-phosphate, and fucose. It therefore allows quantitation of the amount of GDP- ¹⁴ C!fucose remaining at the end of the incubation, and thus provides an estimate of pyrophosphatase activity. Under the standard assay conditions, more than 97% of the GDP- ¹⁴ C!fucose initially present in each mock reaction remained unhydrolyzed and available for transglycosylation.

Each enzyme preparation was also tested for α-fucosidase activity that could hydrolyze the ¹⁴ C!fucosylphenyl-β-D-galactoside product and prevent accurate determination of (α1,2)fucosyltransferase activity. Enzyme preparations were incubated with purified ¹⁴ C!fucosylphenyl-β-D-galactoside (7000 cpm, 10 pmol) in fucosyltransferase assay buffer, but in the absence of added GDP- ¹⁴ C!fucose or phenyl-β-D-galactoside. These reactions were incubated at 37° C. for various times, and were then fractionated by the Sep-Pak method to determine the amount of remaining ¹⁴ C!fucosylphenyl-β-D-galactoside. Fractionated human serum and mouse intestinal mucosa extracts each contained significant α-fucosidase activity. However, inclusion of 10 mM L-fucose in the reaction cocktail effectively inhibited fucosidase activity in these enzyme preparations. Therefore 10 mM L-fucose was included when assaying these enzyme sources. Significant amounts of a-fucosidase activity were not detected in any of the other cell extracts; product hydrolysis never exceeded 1%/h under the standard assay conditions. Reconstruction experiments with these extracts showed that L-fucose at a concentration of 10 mM does not alter the activity of (α1,2)fucosyltransferase.

In aggregate, these experiments showed that, under the conditions used to assay fractionated human serum, human milk, and mouse intestinal mucosa, or crude cell extracts, the quantity of ¹⁴ C!fucosylphenyl-β-D-galactoside product determined reflected true enzymatic activity, because both product hydrolysis and acceptor-independent hydrolysis of the GDP-fucose substrate were negligible.

Digestion of ¹⁴ C!Fucosylphenyl-β-D-galactoside with α-Fucosidase

¹⁴ C!Fucosylphenyl-β-D-galactoside (approximately 10,000 cpm) was digested with 0.025 units of bovine kidney α-L-fucosidase (EC 3.2.1.51, Sigma) in a final volume of 20 μl containing 5 mM sodium citrate pH 6.0, at 37° C. for 1 h. This mixture was then fractionated by descending paper chromatography on Whatman No. 40 in Solvent A for 4 h. The products of the digestion were identified by comparison to parallel separations of L- ¹⁴ C!fucose and ¹⁴ C!fucosylphenyl-β-D-galactoside standards.

Isolation of Human DNA Restriction Fragments from Transfectant Clone s2-2

High molecular weight genomic DNA was isolated from the H-expressing secondary transfectant s2-2, digested to completion with EcoRI, and fractionated through a 1% agarose gel buffered in tris-borate-EDTA. The region of the gel containing the 2.7 kb and the 3.4 kb human EcoRl fragments was divided into 3 mm slices and the DNA in these was isolated by electroelution. Aliquots of the size-fractionated DNA were analyzed by Southern blotting with a radiolabelled Alu probe (BLUR8), using hybridization and wash conditions described above. Fractions containing either the 2.7 kb or the 3.4 kb fragment were used separately to prepare phage libraries in lambda gtll. These libraries were screened with a radiolabelled BLUR8 probe. Positive phages isolated from a tertiary screen were used to prepared phage DNA, and phages containing either the 2.7 kb EcoRI fragment or the 3.4 kb EcoRI fragment were identified by Southern blotting. The 3.4 kb or the 2.7 kb inserts were released from the phage arms by EcoRI digestion, purified by agarose gel electrophoresis and electroelution, and individually subcloned between the EcoRI sites in pWE15.

COS-1 Cell Transfection

Plasmid DNAs were transfected into COS-1 cells by the DEAE-dextran procedure. Seventy two hours after transfection, cells were harvested, extracts were prepared as described above, and extracts were subjected to assays for (α1,2)fucosyltransferase activity, for GDP-fucose hydrolysis activity, and for α-fucosidase activity.

Isolation of Human α(1.2)FT cDNA Clones

1.8×10⁶ recombinant clones from an A431 cell cDNA mammalian expression library were screened by colony hybridization, using a ³² P-labeled 1.2 kb HinfI fragment of pH3.4 as a probe. Filters were hybridized for 18 hours at 42° C. in a hybridization solution described above, washed, and subjected to autoradiography. Two hybridization-positive colonies were obtained and isolated via two additional rounds of hybridization and colony purification. Preliminary sequence analysis of the inserts in both hybridization-positive cDNA clones indicated that they each were in the anti-sense orientation with respect to the pCDM7 expression vector promoter sequences. The largest insert was therefore re-cloned into pCDM7 in the sense orientation for expression studies, and the resulting plasmid was designated pCDM7-α(1,2)FT.

Flow Cytometry Analysis

COS-1 cells were transfected with plasmid DNAs using the DEAE-dextran procedure described above. Transfected cells were harvested after a 72 hour expression period and stained either with mouse IgM anti-H monoclonal antibody (Chembiomed; 10 μg/ml) or with a mouse IgM anti-Lewisa monoclonal antibody (Chembiomed; 10 μg/ml). Cells were then stained with fluorescein-conjugated goat anti-mouse IgM antibody (Sigma; 40 μg/ml) and subjected to analysis by flow cytometry.

Northern and Southern Blotting

A431 poly(A)-plus RNA (10 μg/lane) was subjected to Northern blot analysis. Genomic DNA (10 μg/lane) was subjected to Southern blot analysis. Blots were probed with ³² P-labeled 1.2 kb HinfI fragment of pH3.4.

DNA Sequence Analysis

The insert in pCDM7-α(1,2)FT was sequenced by the method of Sanger using T7 DNA polymerase (Pharmacia) and 20-mer oligonucleotide primers synthesized according to the sequence of the cDNA insert. Sequence analyses and data base searches were performed using the Microgenie Package (Beckman) and the Sequence Analysis Software Package of the University of Wisconsin Genetics Computer Group.

Assay of α(1.2)fucosyltransferase Activity

Cell extracts, conditioned medium from transfected COS-1 cells, and IgG-Sepharose-bound enzyme were prepared and assayed for α(1,2)fucosyltransferase activity by the methods described above. One unit of α(1,2)fucosyltransferase activity is defined as 1 pmol product formed per hour. The apparent Michaelis constant for the acceptor phenyl-β-D-galactoside was determined exactly as described above.

Construction and Analysis of a Protein A-α(1,2)FT Fusion Vector

A 3196 bp StuI/XhoI segment of the cDNA insert containing the putative catalytic domain and 3'-untranslated sequences was isolated from pCDM7-α(1,2)FT. This fragment was blunt-ended using Klenow enzyme and ligated to phosphorylated and annealed oligonucleotides (CGGAATTCCCCACATGGCCTAGG, CCTAGGCCATGTGGGGAATTCCG) designed to reconstruct the coding sequence between the putative transmembrane segment proximal to the StuI site, corresponding to amino acids 33 through 365 of SEQ ID NO:6. The ligated fragment was gel purified, digested with EcoRI and then gel purified again. This EcoRI linkered fragment was ligated into the unique EcoRI site of pPROTA. One plasmid, designated pPROTA-α(1,2)FT,, containing a single insert in the correct orientation, was analyzed by DNA sequencing to confirm the sequence across the vector, linker and insert junctions. Plasmids pPROTA-α(1,2)FTC, PPROTA, pCDM7-α(1,2)FT, or pCDM7 were transfected into COS-1 cells. Following a 72 hour expression period, α(1,2)FT activities in the media, associated with cells, bound to a Sepharose IgG matrix, or to a control Sepharose matrix, were quantitated.

Example II

Cloning and expression of a DNA Sequence Encoding a UDP-Gal:β-D-Gal(1.4)-D-GlcNAc α(1.3)galactosyltransferase (DNA SEQ ID NO:3, Protein SEQ ID NO:4)

A Gene Transfer Approach to Isolate Cloned, Functional, β-D-galactosyl-1,4-N-acetyl-D-glucosaminide α-1,3-galactosyltransferase cDNAs. Tissue- and cell-specific expression of surface-localized terminal Gal(α1-3)Gal linkages is associated with expression of cognate (α1-3)GTs that catalyze a transglycosylation reaction between UDP-Gal and N-acetyllactosamine. COS-1 cells construct surface-expressed polylactosamine molecules that can function as an acceptor substrate for (α1-3)GT but do not express this enzyme or its surface-localized product (see below). The inventor therefore expected that cloned cDNAs encoding an (α1-3)GT would, if expressed in COS-1 cells, generate the surface-localized oligosaccharide product of that enzyme terminal Gal(α1-3)Gal linkages!. Moreover, these particular transfectants could be isolated by virtue of adherence to plates coated with a lectin (GS I-B₄) that specifically binds terminal Gal(α1-3)Gal linkages. A standard transient expression system was used for this approach since it provides for the rescue of transfected cDNAs that determine the expression of cell surface molecules on COS-1 cells and allows the facile construction of large cDNA libraries in a mammalian expression vector.

Isolation of a Cloned cDNA That Determines Expression of GS I-B₄ Binding Activity in Transfected COS-1 Cells. Mouse F9 teratocarcinoma cells express an (α1-3)GT, and this enzyme activity increases concomitant with retinoic acid-induced differentiation of these cells. The inventor therefore prepared a cDNA expression library from retinoic acid-differentiated F9 cells and screened this library for cDNAs that determine expression of GS I-B₄ binding activity in transfected COS-1 cells. One plasmid (pCDM7-αGT) was isolated that, when transfected into COS-1 cells, determined expression of surface molecules that directed specific adherence of cells to culture dishes coated with GS I-B₄. Fluorescence-activated cell sorting analysis confirmed these observations. COS-1 cells transfected with pCDM7-αGT, but not cells transfected with pCDM7, stained brightly with fluorescein isothiocyanate-conjugated GS I-B₄. This staining could be inhibited with raffinose, a hapten for this lectin. These observations indicate that pCDM7-αGT determines de novo expression of surface-localized molecules recognized by GS I-B₄ and thus expression of terminal Gal(α1-3)Gal linkages on cell surface oligosaccharides.

cDNA Sequence Analysis Predicts a Protein with a Transmembrane Topology. The cDNA insert in pCDM7-αGT (SEQ ID NO:3), FIG. 2, is 1500 base pairs long and contains a single long open reading frame in the sense orientation with respect to pCDM7 promoter sequences. Three methionine codons are found within the first 15 codons of this reading frame; the inventor assigned the most proximal of these as the initiator codon, based on Kozak's rules for mammalian translation initiation. This reading frame predicts a protein of 394 amino acids in length (SEQ ID NO:4), FIG. 2, with a molecular mass of 46,472 Da. Hydropathy analysis indicates that this protein has features of a type II transmembrane molecule that is topologically identical to that predicted for two other mammalian glycosyltransferases. This topology predicts a 41-amino-acid, cytoplasmically oriented, NH₂ -terminal segment; a single transmembrane domain consisting of a 19-amino-acid hydrophobic segment flanked by basic residues; and a large (presumably catalytic) COOH-terminal domain that would ultimately be targeted to the lumen of the Golgi. Two potential N-glycosylation sites are present, indicating that this protein, like other glycosyltransferases, may be synthesized as a glycoprotein. This cDNA sequence contains a long 5' untranslated region, with ATG codons at -90 and -251, suggesting that translational control mechanisms may participate in the regulation of expression of this sequence. This is reminiscent of another mammalian glycosyltransferase whose transcript also contains upstream ATG codons. The putative NH₂ -terminal end of this protein lacks a characteristic cleavable signal sequence that may exist in one form of a murine β-1,4-galactosyltransferase.

Searches of the currently available protein and nucleic acid data bases (Protein Identification Resource, Release 21.0 and GenBank, Release 60.0) identified no sequences with significant similarity to the (α1-3)GT DNA sequence, including the sequences of a murine β-1,4-galactosyltransferase and a rat α-2,6-sialyltransferase.

Expression of a Catalytically-Active, Secreted Protein A-(α1-3)GT Fusion Protein. The inventor wished to confirm that this cDNA encodes an (α1-3)GT and to simultaneously exclude the formal possibility that it instead encodes a trans-acting molecule that induces (α1-3)GT activity by interaction with an endogenous gene, transcript, or protein. Therefore, sequences corresponding to the putative catalytic domain of this protein (residues 63-394 of SEQ ID NO:4) were fused in-frame to a secretable form of the IgG binding domain of Staphylococcus aureus protein A in the mammalian expression vector pPROTA yielding the vector pPROTA-αGT_(c). This vector was then tested for its ability to express a catalytically active, secreted and soluble protein A-(α1-3)GT fusion protein.

COS-1 cells transfected with the pCDM7 vector or with the pPROTA vector generated no detectable cell-associated or released (α1-3)GT activity. By contrast, extracts prepared from COS-1 cells transfected with pCDM7-αGT or with the pPROTA-αGT, vector contained 4574 and 20,500 total units, respectively, of (α1-3)GT activity. Moreover, conditioned media prepared from cells transfected with pCDM7-αGT or pPROTA-αGT, contained soluble (α1-3)GT activity amounting to 4,155 units or 50,438 units, respectively. Importantly, the released activity generated by pPROTA-αGT, could be specifically bound to a IgG-Sepharose matrix, whereas the released activity generated by pCDM7-αGT did not interact with this affinity adsorbent. These results indicate that this cloned cDNA encodes an (α1-3)GT, show that information sufficient to generate a catalytically active (α1-3)GT resides within the 332 amino acids distal to the putative transmembrane segment, and show that the catalytic domain can be affinity purified in an enzymatically active state as a portion of a bipartite fusion protein.

Determination of the Structure of the Trisaccharide Product of the (α1-3)GT. Exoglycosidase digestion was used to confirm the α-anomeric linkage predicted for the oligosaccharide product generated by the recombinant enzyme. Radiolabeled trisaccharide product was prepared from UDP- ¹⁴ C!Gal and N-acetyllactosamine by using the IgG-Sepharose-bound enzyme activity generated by pPROTA-αGT_(c). Digestion of the HPLC-purified trisaccharide product with a galactosidase resulted in quantitative release of ¹⁴ C!Gal, whereas the trisaccharide was completely resistant to β-galactosidase digestion.

To confirm that carbon 3 of the galactose in the N-acetyllactosamine acceptor is involved in the glycosidic linkage formed by the recombinant enzyme, the inventor prepared a ³ H!Gal-labeled N-acetyllactosamine acceptor and incubated it with IgG-Sepharose-bound protein A-(α1-3)GT activity and 1 mM UDP-Gal under the standard (α1-3)GT reaction conditions. The trisaccharide product of this reaction was purified and subjected to methylation analysis. Radioactive 2,4,6-trimethylgalactose was identified. Together, these results indicate that the recombinant enzyme can utilize UDP-Gal and N-acetyllactosamine as substrates to construct a trisaccharide product with the structure Gal(α1-3)Gal(β1-4)-GlcNAc.

Northern Blot Analysis. The (α1-3)GT cDNA hybridizes to a single 3.6-kilobase transcript in F9 teratocarcinoma cells. The inventor's DNA sequence analysis of another cloned (α1-3)GT cDNA isolated by colony hybridization indicates that the insert in pCDM7-αGT represents the 5' end of this transcript. The remaining 2.1 kilobases of this transcript consist of 3' untranslated sequence not rescued by the expression cloning procedure.

The specific activity of (α1-3)GT in retinoic acid-differentiated F9 teratocarcinoma cells is approximately 4-fold higher than that in untreated F9 cells. Northern blot analysis indicates that steady-state levels of the (α1-3)GT transcript also increase concomitant with retinoic acid-induced differentiation of F9 teratocarcinoma cells. These results are similar to those reported in F9 cells with β-1,4-galactosyltransferase and suggest that the dynamic changes in cell surface oligosaccharide structures known to accompany in vitro differentiation of this cell line are associated with significant changes in glycosyltransferase gene expression.

Experimental Procedures for Example II, "Cloning and expression of a DNA sequence encoding a UDP-Gal:β-D-Gal(1,4)-D-GlcNAc α(1,3)galactosyltransferase".

Construction of an F9 Cell cDNA Library. A cDNA library was prepared from poly(A)⁺ RNA isolated from retinoic acid-differentiated mouse F9 teratocarcinoma cells by using the procedure of Seed and Arruffo, and the mammalian expression vector pCDM7. pCDM7 is a progenitor of the vector pCDM8; pCDM7 lacks the polyoma sequences present in pCDM8, but is otherwise virtually identical. The library contained 3×10⁶ independent recombinants.

Isolation of a Mouse (α1-3)GT cDNA Clone. Plasmid DNA was prepared from an amplified portion of the library and was transfected in to COS-1 cells by using the DEAE-dextran procedure. Forty samples of 5×10⁵ COS-1 cells (in 100-mm dishes) were transfected with 50 μg of plasmid DNA each. After a 72-hr expression period, the transfected COS-1 cell monolayers were harvested and panned on dishes coated with Griffonia simplicifolia isolectin I B₄ (GS I-B₄). Lectin panning dishes were prepared by using 10 μg of GS I-B4 per ml in phosphate-buffered saline (PBS) containing 0.1 mM Ca²⁺ and 0.1 mM Mn²⁺. Plasmid DNA molecules were rescued from adherent cells and were transformed into the Escherichia coli host MC1061/P3. Plasmid DNA was prepared from these transformants and was subjected to an additional screening by the same procedure. Sib selection was subsequently used to screen for plasmids that determined expression of GS I-B₄ binding activity in COS-1 cells. E. coli transformants containing plasmid molecules rescued from the second screening were plated to yield 16 pools containing between 100 and 5000 colonies each. Plasmid DNAs were prepared from replica plates and were transfected separately into COS-1 cells, and the transfectants were screened by panning on GS I-B₄ -coated dishes. These experiments indicated that approximately 1 out of 1000 colonies contained cloned cDNAs determining the GS I-B₄ -binding phenotype. One "active" ˜1000-colony pool was subdivided into several smaller pools, and these were each tested for GS I-B₄ -binding activity. Three subsequent rounds of sib selection with sequentially smaller, active pools identified a single plasmid (pCDM7-αGT) that directed expression of GS I-B₄ -binding activity in COS-1 cells.

Flow Cytometry

COS-1 cells transfected with plasmid DNAs were harvested 48-72-hr after transfection. These were stained with either fluorescein isothiocyanate-conjugated GS I-B₄ at 10 μg per ml in staining media or with fluorescein isothiocyanate- conjugated GS I-B₄ that had been previously incubated with 50 mM raffinose. Cells were then subjected to analysis by fluorescence- activated cell sorting as described above.

Northern Blotting and DNA Sequence Analysis

Northern blots were hybridized with radiolabeled pCDM7-αGT cDMA insert at 42° C. in hybridization solution. DNA sequencing was performed with the chain termination method by using oligodeoxynucleotides synthesized according to the sequence within the cDNA insert. Sequence data base searches and analyses were performed with the Sequence Analysis Software Package published by the University of Wisconsin Genetics Computer Group.

Assay of (α1-3)GT and Product Characterization

Extracts were prepared from transfected COS-1 cells. Cell extracts, conditioned medium from transfected cells, or IgG-Sepharose-bound enzyme was assayed for (α1-3)GT. One unit of (α1-3)GT activity is defined as 1 pmol of Gal transferred to N-acetyllactosamine acceptor per hour.

HPLC-purified, radiolabeled oligosaccharide reaction products were subjected to digestion with either α-galactosidase (Sigma, 20 mU) or β-galactosidase (Sigma, 1 mU) for 1 hr at 37° C. in buffers recommended by the manufacturer. Reaction products were then fractionated by HPLC. Methylation analysis of reaction product(s) was carried out according to standard procedures.

Construction and Analysis of the Protein A-(α1-3)GT Fusion Vector

A 1050-base-pair segment of the (α1-3)GT cDNA containing the putative catalytic domain was excised from pCDM7-αGT by digestion with EcoRI. This was cloned into the EcoRI site of pPROTA by using a double-stranded linker (5'-ACGGAATTCCGT-3') to maintain the correct reading frame, yielding plasmid pPROTA-αGT_(c).

Plasmids pPROTA-αGT_(c), pCDM7-αGT, and PPROTA were separately transfected into COS-1 cells. After a 72-hr expression period, the media were harvested and subjected to low-speed (300×g for 8 min) and high-speed (100,000×g for 1 hr) centrifugations. Supernatants were then adjusted to 0.05% Tween 20 and were incubated batchwise with 100 μl of preequilibrated IgG-Sepharose or Sepharose 6B overnight at 4° C. The matrices were then thoroughly washed and used directly in (α1-3)GT assays.

Example III

Isolation of a cloned human cDNA that encodes a GDP-Fuc:β-D-Gal (1.4/1.3)-D-GlcNac(/Glc)-α(1.3/1,4)-fucosyltransferase. (DNA SEQ ID NO:1, Protein SEQ ID NO:2)

In one embodiment, the present invention provides a gene transfer system that allows isolating cloned cDNAs encoding functional α(1,3)fucosyltransferase α(1,3)FT! or α(1,4) fucosyltransferase α(1,4)FT! molecules or that otherwise determine α(1,3)FT or α(1,4)FT expression, without the need to first purify the enzyme. This system instead exploits existing reagents that allow detection of the cell surface-expressed oligosaccharide product of these enzymes, and that provide for specific assay of their enzymatic activity.

This approach requires a recipient host cell with specific properties that allow selection of the appropriate cloned cDNA molecules. The host must not express α(1,3)FTs, nor cognate surface Gal β(1,4) Fucα(1,3)!GlcNAc linkages (SSEA-1 structures). However, this host must synthesize the appropriate substrates for surface display of SSEA-1 molecules. These substrates include the nucleotide sugar GDP-fucose, and surface-expressed glycoconjugate molecules that may serve as oligosaccharide acceptors for the transglycosylation reaction. Each of these properties are fulfilled by COS-1 cells.

Fluorescence-activated cell sorter (FACS) analysis indicated that COS-1 cells do not express surface-localized SSEA-1 determinants. Enzyme analyses performed with COS-1 extracts confirmed that absence of SSEA-1 expression was due to a deficiency of α(1,3)FT activity. The inventor expected that COS-1 cells would contain substrate levels of GDP-fucose within their Golgi, since with the exception of certain lectin-resistant mutant cells lines, virtually all mammalian cells synthesize GDP-fucose and translocate it into the Golgi lumen. COS-1 cells also construct surface-expressed glycoconjugates containing unsubstituted polylactosamine moieties that represent potential oligosaccharide acceptors for α(1,3)FT activity determined by a transfected cDNA. The inventor confirmed that these substrates are available for use in the construction of surface-expressed, terminal fucose linkages by demonstrating expression of a different terminally-linked fucose linkage (H Fucα(1,2)Gal) on COS-1 cells after transfection with a cloned human gene fragment that the inventor had previously shown to determine expression of an α(1,2)FT. The inventor therefore observed that COS-1 cells could construct surface-expressed SSEA-1 molecules following transfection with α(1,3)FT-determining cDNAs.

Isolation of a Cloned cDNA That Determines Expression of an α(1,3)FT and Surface-Localized SSEA-1 Structures

The human A431 cell line has been shown to express cell surface Lewis blood group a and b structures that represent the products of an α(1,4)FT. Enzyme assays performed with A431 extracts confirmed that cells also express a corresponding α(1,3)FT activity. A cDNA library was therefore constructed with A431 cell MRNA in the mammalian expression vector pCDM7 and was transfected into COS-1 cells. The transfected cells were screened by the procedure of Seed using a monoclonal antibody specific for SSEA-1 determinants.

In order to follow enrichment for an α(1,3)FT-determining cDNA during the selection procedure, an assay was employed in which 2'-fucosyllactose was used as an acceptor substrate. This acceptor can discriminate between the Lewis α(1,3/1,4)FT and nonallelic human α(1,3)FTs, since it is used efficiently by the former enzyme but not by the latter. With this assay, the inventor was unable to detect any enzyme activity in COS-1 cells transfected with the A431 cDNA library, or in cells transfected with amplified plasmid DNA isolated from the initial selection. However, amplified plasmid DNA obtained from the second selection was found to direct a low level of enzyme activity when transfected into COS-1 cells. A modest increment in enzyme activity was obtained after a third selection by panning. At this stage, "sib selection" was employed to identify and isolate a cloned α(1,3)FT cDNA. Pools of clones isolated from the third panning selection were tested for their ability to generate α(1,3)FT activity in transfected COS-1 cells. From these experiments, it was estimated that approximately one in 500 clones represented a plasmid that determined α(1,3)FT expression. One "active" pool of approximately 400 clones was further subdivided and the resulting pools were tested for their ability to generate enzyme activity in transfected cells. One clone (pCDM7-α(1,3/1,4)FT) in an active 16 clone pool was found to direct very high level expression of the α(1,3)FT. FACS analysis was used to confirm that this plasmid also directs surface expression of SSEA-1 (Lewis x) determinants (FIG. 8). COS-1 cells transfected with this plasmid stain brightly with anti-SSEA-1 antibody, but not with a control IgM anti-H antibody, whereas cells transfected with pCDM7 vector alone exhibit background staining with both antibodies. Identical results were obtained in experiments where the transfected cells were stained with an anti-Lewis a antibody (FIG. 8).

Deduced Protein Sequence of the Cloned cDNA Predicts a Transmembrane Topology

The cDNA insert in pCDM7-α(1,3/1,4)FT (SEQ ID NO:1) is 2022 nucleotides in length, and consists of a 72 bp 5' untranslated region, a continuous open reading frame of 1083 bp, and a 3' untranslated region of 867 bp that is terminated by a poly(A) tail.

This cloned cDNA hybridizes to a single prominent 2.3 kb transcript in A431 cells showing that this insert is essentially full-length. The nature of an additional faint 7.5 kb transcript is at present undefined. The initiation codon at the beginning of the long open reading frame is embedded within a sequence similar to the Kozak consensus initiation sequence and is preceded by two in-frame stop codons. There is also a single additional ATG upstream from the assigned initiator. This ATG also fits the Kozak consensus sequence, but initiates a very short in-frame sequence. The long open reading frame predicts a 361 amino acid protein (SEQ ID NO:2) of Mr 42,069 Da. Sequence comparisons with the latest DNA and protein sequence databases (Protein Identification Resource, Release 21.0, and GenBank, Release 60.0) identified no sequences with significant similarity to this sequence, except for a segment within the 3' untranslated region that is similar to human Alu sequences. The 3' untranslated region also contains 20 degenerate copies of a 16 nucleotide sequence of unknown functional significance.

Comparisons between the sequence predicted by the insert in pCDM7-α(1,3/1,4)FT and four different cloned mammalian glycosyltransferases revealed no obvious primary sequence similarities. While these latter enzymes also share no extensive primary sequence similarities, they exhibit an analogous overall structural organization. Specifically, these enzymes are representative of Type II transmembrane proteins, each composed of a short, NH₂ -terminal cytoplasmic domain, a single transmembrane segment, and a larger, COOH-terminal catalytic domain that ultimately inhabits the Golgi lumen. Inspection and hydropathy analysis of the predicted protein sequence suggested that this protein maintains a similar structural organization. There is a single hydrophobic segment near the amino terminus that is comprised of 19 amino acids and is flanked by basic residues. This putative signal-anchor sequence would place 327 amino acids within the Golgi lumen, while leaving 15 residues within the cytosolic compartment.

The Protein Encoded bv pCDM7-α(1,3/1,4)FT is a Fucosyltransferase

Expression data and the predicted topological similarity of this sequence to other glycosyltransferases, show that this cDNA encodes an α(1,3)FT. Nonetheless, these data are also formally consistent with the possibility that this cDNA sequence instead encodes a molecule that trans-determines α(1,3)FT activity by interaction with an endogenous gene, transcript, or protein. To demonstrate that enzymatic activity is directly associated with this molecule, the putative catalytic domain of the predicted polypeptide (residues 43-361 of SEQ ID NO:2) was fused to a secreted form of the IgG binding domain of Staphulococcus aureus protein A in the mammalian expression vector pPROTA, to generate the vector pPROTA-α(1,3/l,4-Ft),. Since this fusion protein would lack the putative transmembrane anchoring segment of the fucosyltransferase, the inventor expected it would be synthesized as a secreted molecule that could be affinity-purified on an IgG-containing matrix and subsequently tested for α(1,3)FT activity. COS-1 cells transfected with the control vectors pCDM7 or PROTA generated no detectable cell-associated or released enzyme activity. However, conditioned media prepared from COS-1 cells transfected with pPROTA-α(1,3/1,4)FT_(c) or with pCDM7-α(1,3/1,4)FT, contained significant quantities of α(1,3)FT activity. Significantly, virtually 100% of the released activity generated by pPROTA-α(1,3/1,4)FT_(c) was specifically retained by the IgG-Sepharose matrix, and approximately 24% of this activity could be recovered from the matrix after exhaustive washing. By contrast, the released activity generated by pCDM7-α(1,3/1,4)FT did not interact with the affinity adsorbent. These results indicate that the protein encoded by this cloned cDNA encodes a fucosyltransferase, demonstrate that information sufficient to generate α(1,3)FT activity resides within the enzyme's COOH-terminal 319 amino acids, and show that this approach can be used to affinity purify the catalytic domain in an enzymatically active state as a portion of a bipartite fusion protein.

The Fucosyltransferase is a Glvcosylated Transmembrane Protein

In order to confirm the transmembrane topology predicted for the enzyme, fucosyltransferase cRNA was prepared and was subjected to analyses by a series of in vitro translation experiments. The ³⁵ S-methionine-labelled primary translation product generated in these experiments migrated with a molecular weight of approximately 37,500 Da. The discrepancy between this observed molecular weight and the predicted one (42,069 DA) may be reconciled by the observation that membrane-spanning proteins often migrate in an anomalously rapid manner through SDS-polyacrylamide gels, relative to soluble protein molecular weight markers. When this radiolabelled protein was generated by in vitro translation in the presence of canine pancreatic microsomes, it migrated with an Mr of approximately 42,000 Da. The ˜6,000 Da increase in molecular mass observed when the translations were performed in the presence of microsomes suggests that two core glycosylation structures are added by microsomal oligosaccharyltransferase to the two potential asparagine-linked glycosylation sites during cotranslational translocation across the microsomal membrane. This product also co-sedimented with the microsomes, suggesting that the protein had become cotranslationally inserted within, or translocated across, the microsomal membrane. When this raiolabelled, microsome-associated protein was subjected to limit digestion with endoglycosidase H, its molecular mass was reduced to a one essentially identical to that of the primary translation product. Partial endoglycosidase H digestion generated an additional band of intermediate size, that likely consists of molecules that contain a single residual core glycosylation unit. These results indicate that the addition of core oligosaccharide structures is responsible for the increase in size of the protein observed in the co-translation experiments. These observations indicate that the two potential N-glycosylation sites found within the predicted fucosyltranserase amino acid sequence are glycosylated during translocation across the microsomal membrane.

Additional support for the predicted transmembrane topology of the fucosyltransferase was provided by the results of protease protection experiments. Co-translation in the presence of microsomes yields a 42,000 Da polypeptide that is resistant to digestion with protease. The protease-digested product migrates slightly faster than the undigested, microsome-associated polypeptide; this difference is most likely accounted for by removal of some or all of the 15 NH₂ -terminal amino acids predicted to be displayed on the exterior of the microsomes. Addition of microsomes after translation yielded a protease-sensitive, nonglycosylated radiolabelled product, indicating that membrane insertion of the protein is a cotranslational, but not post-translation, event. A small amount of a ˜34KDa polypeptide that is protease-sensitive and glycosylated can also be identified in these experiments. The precise nature of this protein is unknown, but it appears in a proteinase K concentration-dependent manner. The inventor therefore suspected that it represents a proteolytic fragment of the intact protein generated when the integrity of some microsomal vesicles is disrupted. In aggregate, these experiments indicate that the bulk of this polypeptide can be sequestered within the microsomal lumen by a cotranslational translocation process, ultimately yielding a product that is N-glycosylated. These results are consistent with the type II transmembrane topology predicted by the fucosyltransferase sequence.

The Fucosyltransferase Can Construct Two Distinct Glycosidic Linkages

It is believed that, in general, each glycosyltransferase is competent to perform a single transqlycosylation reaction, that in turn generates a single glycosidic linkage. However, genetic and biochemical studies indicate that the human Lewis blood group locus may determine expression of a single fucosyltransferase capable of generating subterminal Fucα(1,3) and Fucα(1,4) linkages on several type I and type II acceptor substrates. In particular, the Lewis enzyme is thought to be the only human fucosyltransferase capable of using the acceptor 2'-fucosyllactose. Since plasmid pCDM7-α(1,3/1,4)FT was isolated with an enrichment scheme involving an enzymatic assay based upon this acceptor substrate, it therefore seemed likely that its cDNA insert encodes the Lewis enzyme. To confirm this, the inventor performed a series of analyses to determine the acceptor specificities of the recombinant enzyme.

Extracts of COS-1 cells transfected with pCDM7-α(1,3/1,4)FT were tested for their ability to catalyze transglycosylations between GDP- ¹⁴ C! fucose and the type I acceptor lacto-N-biose I, or the type II acceptors lactose and N-acetyllactosamine. There are only two possible classes of monofucosylated products that can be formed from each of these acceptors by known human fucosytransferases. These are H-active products containing a Fucα(1,2) linkage on the terminal Gal of these molecules, or Lewis x- or Lewis a-active products containing fucose linked in alpha anomeric configuration to the subterminal monosaccharide of these acceptors via either the monosaccharide's C4 hydroxyl (type I acceptor) or its C3 hydroxyl (type II acceptors). The inventor therefore fractionated the reaction products with a descending paper chromatography method that could distinguish between the two classes of reaction products possible with each acceptor, and thus allow determination of enzyme specificity.

The inventor found that lactose was utilized by the recombinant enzyme to form a radiolabelled compound with the chromatographic mobility characteristic of the Lewis x trisaccharide 3-fucosyllactose, and distinct from the other possible product, the type II H trisaccharide. Likewise, these extracts also generated 3-fucosyl-N-acetyllactosamine when N-acetyllactosamine was used as an acceptor. Radiolabelled fucose was quantitatively released from each product upon digestion with α-fucosidase, indicating that the enzyme attaches fucose to these acceptor substrates in alpha anomeric configuration. These results are consistent with the flow cytometry observations indicating that pCDM7-α(1,3/1,4)FT determines expression of the Galβ(1,4) Fucα1,3!GlcNAc linkage representing the SSEA-1 determinant.

Moreover, the radiolabelled product of the type I acceptor lacto-N-biose I chromatographed with a mobility distinct from the H active standard 2'-fucosyllacto-N-biose I and consistent with its identity as the Lewis a trisaccharide 4-fucosyllacto-N-biose I. This product was also susceptible to digestion with α-fucosidase. Identical results were obtained for all three disaccharide acceptors when affinity-purified protein A-fucosyltransferase was used in these experiments. Taken together, these results indicate that the recombinant fucosyltransferase can construct both Fucα(1,3) and Fucα(1,4) glycosidic linkages on type II and type I disaccharide acceptors, respectively.

In a complementary set of analyses, type I and type II blood group H trisaccharides were tested as acceptors for the enzyme encoded by the fucosyltransferase cDNA. Radiolabelled type I and type II H molecules were prepared by fucosylating their disaccharide precursors at the C2 hydroxyl of their terminal galactose residues, using cell extracts containing the blood group H α(1,2)-FT and GDP ¹⁴ C!fucose. These HPLC-purified radiolabelled type I and type II H acceptors were then each used in reactions containing unlabelled GDP-fucose and affinity-purified fucosyltransferase activity generated by pPROTA-α(1,3/1,4)FT_(c). HPLC analysis of these reactions identified new radiolabelled compounds withchromatographic mobilities predicted for the Lewis b tetrasaccharide and the Lewis y tetrasaccharide, generated with the type I or type II acceptors, respectively. Virtually identical results were obtained with extracts prepared from COS-1 cells transfected with pCDM7-α(1,3/1,4)FT. Results of these experiments and similar ones, are summarized in Table 2.

In a third series of experiments, the inventor demonstrated that this enzyme can operate on "type I" or "type II" acceptors whose terminal galactose residues are substituted with sialic acid in α(2,3) linkage, to generate the sialyl Lewis x and sialyl Lewis a tetrasaccharide determinants. Flow cytometry analysis of COS-1 cells transfected with pCDM7-α(1,3/1,4)FT and stained with a monoclonal anti-sialyl Lewis x antibody indicates that this plasmid can determine surface expression of the sialyl Lewis x antigen (FIG. 8), that is the product of α(1,3)FT action on "type II" acceptors whose terminal galactose residues are substituted with sialic acid in α(2,3) linkage. Likewise, COS-1 cells transfected with pCDM7-α(1,3/1,4)FT and stained with a monoclonal anti-sialyl Lewis a antibody indicates that this plasmid can determine surface expression of the sialyl Lewis a antigen (FIG. 8), that is the product of α(1,4)FT action on type I acceptors whose terminal galactose residues are substituted with sialic acid in α(2,3) linkage. These analyses indicate that the fucosyltransferase encoded by pCDM7-α(1,3/1,4)FT is able to construct two distinct glycosidic linkages on the subterminal Glc or GlcNAc of type I and type II acceptors, and that this does not depend upon the α(1,2)fucosylation or α(2,3)sialylation status of the terminal galactose on these acceptors. These properties mirror those reported for the fucosyltransferase activities determined by human Lewis blood group locus, and confirm that a single fucosyltransferase can catalyze the formation of two distinct glycosidic linkages.

The Fucosyltransferase cDNA Identifies Human Genomic Sequences Syntenic to the Human Lewis Blood Group Locus

Genetic data indicate that the human Lewis blood group is determined by a locus on chromosome 19. The fucosyltransferase cDNA was therefore used for Southern blot analysis of a pair of human-mouse somatic cell hybrids that differ only by the presence or absence of human chromosome 19. The results indicate that at high stringency, the fucosyltransferase cDNA identifies cross-hybridizing sequences located on chromosome 19. Taken together with the enzymatic analyses, these data strongly suggest that this cloned cDNA represents the product of the human Lewis blood group locus.

Experimental Procedures for Example III, "Isolation of a cloned human cDNA that encodes a GDP-Fuc:β-D-Gal (1,4/1,3)-D-GlcNAc(/Glc)-α(1,3/1,4)-fucosyltransferase".

cDNA Library Construction

A cDNA library was prepared from poly(A)-plus RNA isolated from human A431 cells, using standard procedures and the mammalian expression vector pCDM7. pCDM7 lacks polyoma sequences present in the vector pCDM8, but is otherwise virtually identical. The library contained 2.6×10⁶ independent recombinants.

Cell Lines

Mouse 3T3-human hybrid cell lines KLEJ-47 and KLEJ-47/P-1 were obtained from Dr. Howard Green (Harvard University, Boston). Mouse 3T3 cells were from Dr. Vishva Dixit (University of Michigan, Ann Arbor). The origins of all other cell lines, and conditions for cell growth are as previously described in the literature.

Preparation of Panning Dishes

Panning dishes were prepared by first coating them with goat anti-mouse IgM, and then with monoclonal anti-SSEA-1 antibody (ascites generously provided by D. Solter, diluted 1:1000).

cDNA Library Screening

The A431 library was screened as described previously. Plasmid DNA was rescued from transfected COS-1 cells adherent to panning dishes and introduced into the bacterial host MC1061/P3 by transformation. Transformants were grown to saturation in liquid culture under antibiotic selection, aliquots were removed for frozen storage, and the remainder of the culture was used to prepare plasmid DNA. A portion of this plasmid DNA was used for subsequent enrichment by transfection and immunoselection on anti-SSEA-1 panning dishes.

FACS Analysis

Transfected COS-1 cells were stained with the mouse IgM anti-SSEA-1 (anti-Lewis x) monoclonal antibody (1:1000 dilution of ascites) mouse monoclonal IgM anti-H or anti-Lewis a antibodies (Chembiomed, Ltd., Edmonton; 10 μ/ml), a mouse monoclonal IgM anti-sialyl Lewis x antibody (CSLEX, P. Terasaki, 10 μg/ml), or a mouse monoclonal IgG anti-sialyl Lewis a antibody (CSLEA, P. Terasaki, 1:1000 dilution of ascites). Cells were then stained with fluorescein-conjugated goat anti-mouse IgM (Sigma; 40 μg/ml) and subjected to analysis by fluorescence activated cell sorting as described previously in the literature by the inventor.

Northern and Southern Blotting

A431 cell RNA was subjected to Northern blot analysis as previously described. The probe consisted of a 1.7 kb Xhol - Xbal fragment isolated from the 5' end of the cDNA insert in plasmid pCDM7-α(1,3/1,4)FT. This fragment does not contain the portion of this cDNA that exhibits sequence similarity to human Alu sequences. This probe was labelled by nick translation with α ³² P!dCTP to a specific activity of 6×10⁸ cpm/μg.

Genomic DNA was prepared and subjected to Southern blotting as described previously. Blots were subjected to a final wash for 30 minutes at 65° C. in O.1X SSC, 0.5% SDS. The probe used was identical to the one used for Northern blot analysis except that it was labelled with the random priming method.

Sequencing

The cDNA insert in plasmid pCDM7-α(1,3/1,4)FT was sequenced by the chain termination method using a double stranded plasmid DNA template and commercial reagents (Pharmacia). Both strands were sequenced using 17-mer or 19-mer oligonucleotide probes synthesized according to the sequence of the cDNA insert. The DNA sequence was assembled and analyzed using the Beckman Microgenie package, and the Sequence Analysis Software Package of the University of Wisconsin Genetics Computer Group.

In Vitro Transcription-Translation

Plasmid pCDM7-α(1,3/1,4)FT DNA was linearized downstream from the cloned cDNA insert by digestion with Notl. Capped RNA transcripts were then generated from this linearized template using a T7 polymerase promoter based in vitro transcription kit (Pharmacia). Transcripts initiate from the T7 promoter proximal to the cDNA cloning site in pCDM7. RNA transcripts produced in vitro were used to program a rabbit reticulocyte lysate in vitro translation system (Promega), in the presence of ³⁵ S-methionine (Amersham), according to the manufacturer's instructions. Membrane-associated radiolabelled in vitro translation products, generated in the presence of canine pancreatic microsomal membranes (Promega) (cotranslation) or generated prior to the addition of microsomes (post-translational microsome addition), were isolated from the bulk of the soluble reaction components by centrifugation through a sucrose cushion (0.5 M sucrose, 10 mM Tris 7.4, 150 mM NaCl; 170,000×g for 60 min). For endoglycosidase H digestions, pellets containing microsome- associated radiolabelled products were first resuspended in 50 mM sodium citrate pH 5.5, were made 0.2% in SDS, and were heated to 100° C. for 4 minutes. Aliquots of this material were then diluted with an equal volume of water and subjected to digestion with either 10 mU or 5 mU of endoglycosidase H for 20 hrs at 37° C., in the presence of 0.1% BSA, 0.5% Triton X-100, 0.5 mM PMSF, 40 μg/ml Bestatin, 10 μg/ml α₂ macroglobulin, and 30 μg/ml of E-64. Alternatively, the pellets were resuspended in ice cold in vitro translation buffer containing 5 mM CaCl₂, and were subjected to incubation with 150 μg/ml proteinase K, on ice for 1 hour, in the presence or absence of 1% Triton X-100. The various radiolabelled in vitro translation products were then denatured and reduced by heating to 100° C. for 4 minutes in 62.5 mM Tris pH 6.8, 100 mM dithiothreitol, 2% SDS, 10% glycerol, and 0.02% bromphenol blue. Samples were then fractionated through SDS polyacrylamide gels, and the gels were subjected to autoradiography.

Fucosyltransferase Assays

Cultured cells were washed in PBS, harvested by scraping with a rubber policeman, washed again in PBS, and pelleted by centrifugation. Cell extracts were prepared by resuspending cell pellets in 1% Triton X-100 such that the final protein concentration in the extracts was approximately 5 mg/ml (BCA method, Pierce Chemical Co.).

Fucosyltransferase assays were performed in 50 mM MOPS pH 6.5, 25 mM MnCl₂ 10 mM L-fucose, 5 mM ATP, 3 mM GDP- ¹⁴ C!fucose (specific activity of 600,000 cpm/nmol; 35,000 cpm per assay), 2.5 mM acceptor (e.g. 2'-fucosyllactose, N-acetyllactosamine, lactose or lacto-N-biose I), and up to 10 μl of cell extract, in a final volume of 20 μl. When determining α(1,3)FT specific activities achieved during the sib selection process, and in the analysis of the protein A-fucosyltransferase fusion protein experiments, the amount of added cell extract and incubation times were adjusted to yield (linear) reaction rates reflecting accurate specific activities. Reactions were incubated at 37° C. for 2 or 6 hours, and then terminated by the addition of 20 μl of ethanol, followed by dilution with 500 μl of H₂ O. The terminated, diluted reactions were then centrifuged at 15,000×g for 5 min. Fifty μl of each reaction supernatant was counted to determine total radioactivity, and 200 μl of each was fractionated by Dowex-1 chromatography. The neutral radiolabelled material eluting from the column was then counted directly as a measure of product formation. Enzyme specific activity is defined as pmol of fucose transferred from GDP-fucose to acceptor per mg cell extract protein per hour. Neutral products were also further analyzed as described below by descending paper chromatography and by HPLC to confirm their identity. Parallel reactions were done in the absence of added acceptor to allow correction for transfer to endogenous acceptor molecules and for substrate and product hydrolysis. These control experiments indicated that less than 2.6% of the radioactivity in GDP- ¹⁴ C!fucose was found as a neutral product in the absence of added acceptor, and that virtually all of this material represented ¹⁴ C! fucose.

In instances where radiolabelled, H type I and H type II molecules were used as acceptors, nonradiolabelled GDP-fucose was included instead of GDP- ¹⁴ C fucose, and reactions were allowed to proceed for 16 hours. Residual unreacted neutral radiolabelled acceptor substrate, and neutral radiolabelled product were isolated by Dowex-1 chromatography and then analyzed by HPLC.

Preparation of Radiolabelled H Type I and H Type II Acceptors

Cell extracts containing a human α(1,2)FT activity were used to synthesize radiolabelled type I H or type II H acceptor molecules. The cell extracts were prepared from mH1-12 cells, a mouse L cell transfectant containing a human DNA segment that encodes a human α(1,2)FT. These extracts contain no detectable α(1,3)FT activity or α(1,4)FT activity. Lacto-N-biose I (20 mM), or N-acetyllactosamine (20 mM), were incubated with 100 μg of mH1-12 extract protein in 40 μl of 25 mM sodium cacodylate pH 6.2 containing 3 μM GDP- ¹⁴ C!fucose, for 16 hours at 37° C. Reactions were terminated by the addition of 40 μl of ethanol followed by dilution with 200 μl of water. Precipitated protein was removed by centrifugation at 12,000×g for 5 minutes, and the neutral radiolabelled reaction products in the supernatant were isolated by Dowex-1 chromatography. The type I H trisaccharide molecules (lacto-N-biose I reaction) or type II H trisaccharide molecules (N-acetyllactosamine reaction) comprising the majority of the respective neutral radiolabelled materials were then purified by HPLC as described below.

Product Analysis bv HPLC and Descending Parer Chromatography

Neutral radiolabelled reaction products generated by affinity-purified protein A-fucosyltransferase fusion protein, or by pCDM7-α(1,3/1,4FT)-programmed COS-1 extracts, type I or type II disaccharide acceptors, and GDP- ¹⁴ C!fucose (see above, Fucosyltransferase Assays) were fractionated by descending paper chromatography or by HPLC chromatography to determine their structures. Samples analyzed by HPLC were dissolved in 70% acetonitrile and applied to a Dynamax 60A (primary amine column, Rainin Instruments, 4.14 mm×25 cm) equilibrated in acetonitrile:water (70:30). Compounds were eluted with a linear gradient of acetonitrile:water (70:30 to 40:60), in 1 hour, at a flow rate of 1 ml per minutee. The eluant was monitored with a Beckman Instruments on-line radioisotope detector.

Samples analyzed by descending paper chromatography were dissolved in water and fractionated through Whatman No. 40 in phenol/isopropanol/formic acid/water (85:5:10:100, lower layer). After chromatography (FIG. 6; 40 hours in panel A or 48 hours in panel B), air-dried chromatograms were cut into 1 cm strips and the radiolabelled compounds were eluted into 2 ml of water. The radioactivity in each eluate was determined by scintillation counting after mixing with 10 ml of scintillation cocktail. HPLC-purified ¹⁴ C-labelled type I and type II H-active trisaccharide standards were prepared as described above for the preparation of ¹⁴ C-labelled type I and type II H-active acceptor trisaccharides.

α-L-Fucosidase Digestion

Neutral, HPLC-purified, radiolabelled fucosyltransferase products were subjected to α-L-fucosidase digestion to confirm the alpha anomeric configuration of the attached fucose. (1,3)(¹⁴ C!fucosyl-N-acetyllactosamine, (1,3) ¹⁴ C!fucosyl-2'-fucosyllactose, (1,3) ¹⁴ C!fucosyllactose, and (1,4) ¹⁴ C!fucosyllacto-N-biose I were purified by HPLC, and aliquots of each (10,000 to 20,000 cpms) were digested with 100 mU of a-L-fucosidase (E.C. 3.2.1.51, Boehringer-Mannheim) in 70 μl of 100 mM Na citrate pH 5.5, at 37° C. for 22 hrs. The reactions were desalted by Dowex column chromatography and subjected to HPLC analysis using conditions described above. The products of the digestion were identified by comparison to parallel separations of L- ¹⁴ C!fucose and ¹⁴ C!fucose-labelled acceptors. In each case, quantitative release of L- ¹⁴ C!fucose was achieved by α-L-fucosidase digestion.

pPROTA-α(1,3/1,4)FT, Construction and Analysis

A 1344-bp Smal-BamHl segment of the cDNA insert containing the putative fucosyltransferase catalytic domain was isolated from pCDM7-α(1,3/1,4)FT. This fragment was blunt-ended with the Klenow fragment of DNA polymerase I, and the ends were ligated to kinased double stranded linkers (5' CGGAATTCCG 3'). The ligated fragment was gel purified, digested with EcoRI, and gel purified again. This fragment was inserted at the unique EcoRI site of PPROTA. One plasmid (pPROTA-α(1,3/1,4)FT_(c)) containing a single insert in the appropriate orientation was analyzed by DNA sequencing to confirm the predicted sequence across the junctions between the vector, linker, and insert.

Plasmids pPROTA-α(1,3/1,4)FT_(c), pCDM7-α(1,3/1,4)FT, or pPROTA, (50 μg each) were separately introduced into COS-1 cells (500,000 per 100 mm dish) by DEAE-dextranmediated transfection. After a 72-hour expression period, the media (10 ml) was harvested from each plate and subjected to low speed (300×G for 8 min) and high speed (100,000×G for 1 h) centrifugations. The supernatants were then adjusted to 0.05% Tween 20 and were either assayed directly, or were used in IgG-Sepharose binding studies. IgG-Sepharose or Sepharose 6B were preequilibrated as described by the manufacturer (Pharmacia), and then equilibrated in 10% fetal calf serum in Dulbecco's modified Eagle's medium (FCS/DMEM). Aliquots of processed supernatants containing known amounts of enzyme activity prepared from transfected COS-1 cells were incubated batchwise with 100 μl of equilibrated IgG-Sepharose or Sepharose 6B, overnight at 4° C. Supernatants were saved for assay ("Flow thru" activity). The matrices were then washed by centrifugation, 9 times with 1 ml of 50 mM Tris pH 7.5, 1 mg/ml bovine serum albumin, twice with 1 ml of 20 mM Tris pH 7.5, 5 mM CaCl₂, 0.05% Tween-20, and once with FCS/DMEM. The matrices were then resuspended in an equal volume of FCS/DMEM. This suspension was used directly for assay of α(1,3)FT activity.

Example IV

Cloning by cross-hybridization, and expression, of a DNA sequence encoding GDP-FuC:β-D-Gal(1,4)-D-GlcNAc α(1,3)fucosyltransferase (Fuc-TIV) (DNA SEQ ID NO:7. Protein SEQ ID NO:8).

The inventor had previously used a mammalian gene transfer procedure to isolate a clone cDNA (SEQ ID NO:1) that encodes the human Lewis blood group fucosyltransferase (SEQ ID NO:2). The inventor was aware of biochemical and genetic data indicating that the human genome contains two or more other structural genes that encode fucosyltransferases competent to construct surface localized Lewis x determinants (Galβ1→4 Fucα(1→3)!GlcNAc-). These other enzyme(s) were thought to be polypeptides distinct from the Lewis fucosyltransferase because they exhibit different acceptor substrate specificities and differential sensitivities to divalent cation and N-ethylmaleimide inactivation. Moreover, their expression is determined by loci distinct from the Lewis blood group fucosyltransferase locus, and they display tissue specific patterns that are different from expression patterns determined by the Lewis locus. Because these enzymes exhibit properties that are very similar to the Lewis blood group fucosyltransferase, the inventor considered it possible that their corresponding genes might be sufficiently related at the primary sequence level to be able to isolate them by cross-hybridization approaches. He considered this even though he and others had previously shown that glycosyltransferase sequences that use the same substrates are not at all related in their primary nucleic acid or amino acid sequences, since he knew that the fucosyltransferases exhibited very similar substrate requirements, and in each case constructed one or more oligosaccharide products identical to those made by the Lewis fucosyltransferase.

In consideration of the possibility that these α(1,3)fucosyltransferases might be encoded by a family of structurally-related genes, he sought to isolate other such members by cross-hybridization methods, using the cloned Lewis fucosyltransferase cDNA. Low stringency Southern blot hybridization experiments indicate that the coding region of the Lewis α(1,3)fucosyltransferase cDNA detects strongly hybridizing human DNA restriction fragments, as well as several weakly hybridizing fragments. Weakly hybridizing fragments were always detected regardless of the restriction enzyme used, suggesting that these represented one or more DNA sequences distinct from the authentic Lewis gene presumably represented by the strongly-hybridizing fragments. To further examine the molecular nature of these sequences, he screened a human lambda phage genomic DNA library at low stringency with the Lewis cDNA probe. A total of 18 phages were isolated from phages representing approximately five human genomic equivalents. Southern blot analysis of 16 of these phages allowed them to be placed into three groups, based upon their restriction patterns and hybridization signal intensity strengths. Six phages representing a class of intermediate hybridization intensity were identified. A 3.6 kb cross-hybridizing PstI restriction fragment was subcloned from a representative phage of this class. To determine the relationship of this fragment to cross-hybridizing fragments detected in human genomic DNA with the Lewis probe, a 400 bp AvaII-PvuII segment of this fragment, that cross-hybridized with the Lewis coding sequence probe, was also used to probe Southern blots at low stringency. For each enzyme used to generate the Southern blots, the AvaII-PvuII probe detected one strongly hybridizing fragment, and one or more weakly hybridizing fragments. Each strongly hybridizing fragment corresponded to one of the weakly hybridizing fragments generated by the same enzyme and detected by the Lewis probe. Likewise, the strongly hybridizing fragments detected with the Lewis probe correspond to fragments that exhibit weak hybridization to the AvaII-PvuII probe. These results suggested that this probe, and the 3.6 kb fragment from which it was derived, represented the weakly cross-hybridizing DNA sequences detected by the Lewis probe on genomic DNA Southern blots.

The homologous DNA restriction fragment maintains a single long open reading frame that predicts a polypeptide with similarity to the Lewis blood group α(1,3/1,4)fucosyltransferase cDNA

DNA sequence analysis of the 3.6 kb PstI fragment (SEQ ID NO:7) identified a single long open reading frame within its 3' portion corresponding to sequences that cross-hybridized to the Lewis cDNA probe (FIG. 4 and FIG. 5). This reading frame begins with a methionine codon that is found within a sequence context consistent with Kozak's consensus rules for mammalian translation initiation. Moreover, hydropathy analysis of the protein sequence predicted by this reading frame indicates a single hydrophobic segment at its NH₂ -terminus, suggesting that the predicted polypeptide (SEQ ID NO:8) would maintain the type II transmembrane orientation typical of mammalian glycosyltranferases. The distal portion of this reading frame shares a substantial amount of amino acid sequence identity with the corresponding portion of the Lewis fucosyltransferase (FIG. 5). These sequences share the highest degree of similarity between their COOH-terminal portions, within the catalytic domain of the Lewis fucosyltransferase. Sequence divergence occurs toward the predicted NH₂ -end, within the "stem" and transmembrane regions of the latter enzyme.

The DNA restriction fragment detects mRNA transcripts in HL-60 myeloid cells

To test the possibility that this segment represents a functional α(1,3)fucosyltransferase gene, a portion of it was used as a probe to identify transcripts in a cell line known to express such enzymes. The HL-60 human cell line was examined since these myeloid lineage cells are known to express one or more α(1,3)fucosyltransferases that are distinct from the Lewis α(1,3)fucosyltransferase. Northern blot analysis of polyadenylated mRNA isolated from these cells, using the 400 bp AvaII-PvuII segment corresponding to a portion of the open reading frame, identified four distinct transcripts. By contrast, no transcripts were detected when the same analysis was preformed using the Lewis cDNA. These results are consistent with the possibility that the fucosyltransferase(s) expressed by HL-60 cells are encoded by the open reading frame in the cloned PstI segment.

The open reading frame in the homoloaous DNA restriciton fracment determines expression of an α(1,3)fucosyltransferase.

To determine it this segment encodes an α(1,3)fucosyltransferase, the 3.6 kb PstI fragment was cloned into a mammalian expression vector and the resulting plasmid (pCDNA1-Fuc-TIV, "Experimental Procedures") was introduced into two types of mammalian host cells by transfection. Transfected cells were then analyzed for vector-dependent cell surface glycoconjugate expression and for fucosyltransferase activity. COS-1 cells and CHO cells were used as hosts for these experiments since neither cell line normally expresses any detectable α(1,3)- and α(l,4)fucosyltransferase activities. Likewise, COS-1 and CHO cells do not normally express detectable amounts of cell surface Galβ1→4 Fucα(1→3)!GlcNAc-(Lewis x, SSEA-1) moieties, or the α2→3 sialylated derivative (NeuAcα2→3Galβ1→4 Fucα(1→3)!GlcNac-, sialyl Lewis x). These cells do, however maintain surface-display of the non-fucosylated neutral and α2→3-sialylated type II oligosaccharides that can function as precursors to such molecules, via the action of the α(1,3)fucosyltransferase encoded by a transfected Lewis cDNA expression vector. COS-1 cells also maintain surface display of the type I precursors to the Lewis a (Galβ1→3 Fucα(1→4)!GlcNAc-) and sialyl Lewis a (NeuNAcα2→3Galβ1→3(Fucα(1→4)!GlcNAc-) moieties. The vector pCDNAI was used since this plasmid efficiently transcribes exogenous, subcloned sequences in mammalian hosts by virtues of the cytomegalovirus immediate early promoter sequences in the vector.

In initial biochemical analyses, extracts prepared from COS-1 cells transfected with plasmid pCDNAI-Fuc-TIV were tested for the presentce of vector-dependent fucosyltransferase activity, using several low molecular weight acceptor substrates. In a standard fucosyltransferase assay ("Experimental Procedures"), extracts prepared from pCDNA1-Fuc-TIV transfected cells, but not from control transfectants, contained a fucosyltransferase activity (296 pmol/mg-h) that utilized the type II disaccharide acceptor N-acetyllactosamine to yield a radiolabeled product with a chromatographic mobility ("Experimental Procedures") characteristic of authentic Galβ1→3 Fucα(1→4)!GlcNAc (R_(2'-fucosyl-N-acetyllactosamine) =0.85). However, under these assay conditions, two other neutral type II molecules (2'fucosyllactose, lactose) did not function as efficiently as N-acetyllactosamine as acceptor substrates for the fucosyltransferase in these extracts (17 and 10 pmol/mg-h, respectively, for 2'-fucosyllactose and lactose). Only a trace amount of transfer could be detected using the type I substrate lacto-N-biose I.

Likewise, the inventor did not detect fucose transfer to the sialylated acceptor NeuAcα(2→3)Galβ(1→4)GlcNAc (less than 1 pmol/mg-h), even in extracts that exhibited a relatively large amount of activity toward N-acetyllactosamine (474 pmol/mg-h). By contrast, under these same conditions, extracts containing the Lewis blood group fucosyltransferase utilized both the sialylated acceptor (297 pmol/mg-h) and N-acetyllactosamine (526 pmol/mg-h), to form, respectively, the sialyl Lewis x tetrasaccharide and the Lewis x trisaccharide (see "Experimental Procedures"). Thus, the restricted acceptor preference exhibited by this enzyme in vitro contrasts remarkably with that exhibited by the Lewis α(1,3/1,4)fucosyltransferase, which can efficiently utilize each of the five acceptors tested. These results are summarized in Table 2.

COS-1 cells transfected with pCDNA1-Fuc-TIV were also analyzed by flow cytometry to detect de novo, vector-dependent surface expression of these oligosaccharide products, to allow an assessment of the enzyme's in vivo acceptor substrate requirements. The transfected COS-1 cells exhbited positive staining with a monoclonal antibody directed aginst the Lewis x moiety Galβ1→4 Fucα(1→3)!GlcNAc-) (FIG. 8), whereas cells transfected with the pCDNA1 vector without insert did not express this determinant. However, COS-1 cells transfected with pCDNA1-Fuc-TIV, or with its control plasmid, did not stain with antibodies specific for the sialyl Lewis x antigen (FIG. 8). Likewise, the transfected cells did not exhibit detectable surface expression of Lewis a or sialyl Lewis a molecules (FIG. 8).

Polylactosaminoglycans with terminal α(2→3)-linked sialic acid also exist that maintain a single internal α(1,3)-linked fucose on the N-acetylglucosamine residue of the pneultimate lactosamine repeat. This determinant (NeuAcα2→3Galβ1→4GlcNAcβ1→4 Fuc.alpha.(1→3)!GlcNAc-) can be detected on the surfaces of myeloid cells by the monoclonal antibody VIM-2, and may be constructed by the action of α(1,3)fucosyltransferase(s) on type II polylactosamine acceptors whose terminal galactose residues are substituted with α(2,3)sialic acid moieties. Neither COS-1 cells transfected with the Lewis α(1,3/1,4)fucosyltransferase, nor COS-1 cells tranfected with plasmid pCDNA1-Fuc-TIV display detectable amounts of this determinant.

Virtually identical results were obtained with COS-1 cells transfected with the plasmid pCDNAI-α(1,3)FTMlu ("Experimental Procedures"). This vector encompasses sequences corresponding to bp-1904 through the end of the open reading frame in FIG. 4. These results provide additional evidence for the hypothesis that the open reading frame displayed in FIG. 4 corresponds to the coding portion of this fucosyltransferase gene.

To further demonstrate that enzymatic activity is directly associated with this protein, the putative catalytic domain of the predicted polypeptide (amino acids 50 to 405 of SEQ ID NO: 8) was fused to a secreted form of the IgG binding domain of Staphylococcus aureus protein A in the mammalian expression vector pPROTA, to generate the vector pPROTA-α(Fuc-TIV)_(c). Since this fusion protein would lack the putative transmembrane anchoring segment of the fucosyltransferase, the inventor expected it would be synthesized as a secreted molecule that could be affinity-purified on an IgG-containing matrix and subsequently tested for α(1,3)FT activity. COS-1 cells transfected with the control vectors pCDM7 or pPROTA generated no detectable cell-associated or released enzyme activity. However, conditioned media prepared from COS-1 cells transfected with pPROTA-α(Fuc-TIV), contained significant quantities of α(1,3)FT activity when assayed with N-acetyllactosmine. Virtually 100% of the released activity generated by pPROTA-α(Fuc-TIV), is specifically retained by the IgG-Sepharose matrix. These results indicate that the protein encoded by this cloned DNA segment encodes a fucosyltransferase, and demonstrate that information sufficient to generate α(1,3)FT activity resides within the enzyme's COOH-terminal 356 amino acids.

Biochemical analysis of extracts prepared from a CHO cell line transfected with pCDNA1-Fuc-TIV (CHO-FT3 cells) yielded results similar to those obtained with the transfected COS-1 cells. In the standard fucosyltransferase assay (Experimental Procedures), extracts prepared from the control transfected cell line CHO-V did not contain α(1,3)fucosyltransferase activity when tested with N-acetyllactosamine, 2'-fucosyllactose, lactose, or lacto-N-biose I, or NeuAcα(2→3)Gal(1→4)GlcNAc. By contrast, extracts prepared from the CHO-FT3 line contained an α(1,3)fucosyltransferase activity (59.1 pmol/mg-h) that utilized the type II disaccharide acceptor N-acetyllactosamine to yield a radiolabeled product characteristic of authentic Galβ(1→4) Fucα(1→3)!GlcNAc (R_(2'-fucosyl-N-acetyllactosamine) =0.85) (see "Experimental Procedures"). Under these assay conditions, the CHO-FT3 extracts utilized the type II acceptor molecules 2'-fucosyllactose and lactose with substantially lower efficiency (5.8 pmol/mg-h and 2.0 pmol/mg-h, respectively). Virtually no transfer could be detected when these extracts were tested with the type I substrate lacto-N-biose I (<1 pmol/mg-h) or with the sialyl Lewis x precursor NeuNAcα2→3Galβ1→4GlcNAc (<1 pmol/mg-h). These results confirm those obtained with extracts prepared from the transfected COS-1 cells, and indicate that, to a first approximation, the COS-1 and CHO genetic backgrounds do not strongly influence the enzyme's ability to utilize these five low molecular weight acceptor substrates.

With one striking and important exception, flow cytometry analyses with the CHO-FT3 cells were virtually identical to those obtained with the transfected COS-1 cells. CHO-FT3 cells exhibit uniform, bright staining with anti-Lewis x antibody, but not with antibody directed against the sialyl Lewis x molecule. Control transfected cells do not stain with either antibody. As expected, neither cell line stained with antibodies against the neutral and α2→3-sialylated Lewis a isomers, since CHO cells do not construct type I precursors. However, these cells differed in an important way from the transfected COS-1 cells, in that, like CHO cells transfected with the Lewis α(1,3/1,4)fucosyltransferase cDNA (pCDM7-α(1,3/1,4)FT), these cells expressed substantial amounts of the VIM-2 determinant.

Taken together with the results of the biochemical analyses performed with extracts from the transfected cells and summarized in Table 2, the flow cytometry analyses presented in FIG. 8, the protein A gene fusion experiments, and the DNA sequence analysis indicate that plasmid pCDNA1-Fuc-TIV encodes an α(1,3)fucosyltransferase. Transfection results obtained with plasmid pCDNAI-α(1,3)FTM1u also demonstrate that this enzyme is encoded by the open reading frame displayed in FIG. 4. The results further indicate that this enzyme can utilize type II precursors, but not type I precursors, and suggest that the enzyme cannot efficiently utilize α2→3-sialylated type II glycoconjugates to form the sialyl Lewis x determinant.

Experimental Procedures for Example IV, "Cloning by cross-hybridization and expression, of a DNA sequence encoding GDP-Fuc:β-D-Gal(1,4)1-D-GlcNAc α(1,3)-Fucosyltransferase: (Fuc-TIV)"

Cell Culture

The source and growth conditions of COS-1 cells, CHO transfectants, and A431 cells are as previously described (Ernst et al, J. Biol. Chem. (1989) 265:3436-3447; Rajan et al, J. Biol. Chem. (1989) 264:11158-11167). The human HL-60 cell line was obtained from Dr. Steve Kunkel (University of Michigan, Ann Arbor). HL-60 cells were grown in 10% fetal calf serum and Dulbeccols Modified Eagle's Medium.

Antibodies

The anti-Lex antibody anti-SSEA-1 Solter et al, Proc. Nat. Acad. Sci. (USA) (1978) 75:5565-5569) (mouse monoclonal IgM as ascites) was used. Anti-H and anti-Lewis a antibodies (mouse monoclonal IgM, antigen affinity purified) were purchased from Chembiomed Ltd. (Edmonton, Alberta). Anti-sialyl Lewis x antibody CSLEX1 (Fukushima et al, Cancer Res. (1984) 44:5279-5285) (mouse monoclonal IgM, HPLC purified) and anti-sialyl Lewis a antibody CSLEA1 (Chia et al, Cancer Res. (1985) 45:435-437) (mouse monoclonal IgG3, ammonium sulfate precipitate) were used. Anti-VIM-2 was obtained from Dr. Bruce Macher (San Francisco State University). A pooled mouse IgG antibody preparation (MsIg) was purchased from Coulter. Fluorescein-conjugated goat anti-mouse IgM or IgG antibodies were purchased from Sigma.

Human Genomic Library Construction

High molecular weight human genomic DNA was prepared from peripheral blood leukocytes as described previously (Ernst et al (1989)). Genomic DNA was subjected to partial digestion with the restriction endonuclease Sau3A. The partially digested genomic DNA was size fractionated by ultracentrifugation through a sodium chloride gradient. Fractions enriched for DNA fragments between 8 Kb and 20 Kb were ligated to XhoI digested lambda FIX (Stratagene) phage arms that had been partially filled in with dTTP and dCTP to make the ends compatible with the Sau3A fragments. The ligation mixture was packaged in vitro with commercial packaging extracts (Stratagene), titered on E. coli host TAP90 (Patteron et al, Nucl. Acids Res. (1987) 15:6298). Approximately 1.0×10⁶ recombinant lambda phage were screened by plaque hybridization. Plaque lifts were prepared using nitrocellulose filters (Schleicher and Schuell) and were prehybridized at 42° C. for 16 hours in 50% formamide, 5X SSC, 10X Denhart's solution, and 0.1% SDS. Filters were hybridized for 72 hours at 35° C. in prehybridization solution containing 10% dextran sulfate, and 100 micrograms per ml denatured salmon sperm DNA. The probe consisted of a 1.7 Kb XhoI-XbaI fragment isolated from the 5' end of a cDNA insert encoding the Lewis blood group α(1,3/1,4) fucosyltransferase which was labeled with α-³² P! dCTP. The filters were rinsed three times for 20 minutes each at room temperature in 2X SSC and then once for 40 minutes at 50° C. and 1X SSC, 0.5% SDS. Filters were then subjected to autoradiography. Eighteen independent hybridization-positive plaques were identified after 2 additional cycles of plaque hybridization. Phage DNAs were prepared from liquid lysates and were subsequently characterized by restriction endonuclease digestions and Southern blot analyses.

DNA Sequence Analysis

Phage DNA was digested with PstI and a 3.8 Kb fragment homologous to the human α(1,3/1,4)fucosyltransferase cDNA was gel purified and ligated into the PstI site of pTZ18R. A representative subclone containing a single insert was designated pFT-3. A 970 bp hybridization-positive AvaII-PstI fragment was isolated from insert in pFT-3 and subcloned into pTZ18R. The DNA sequence of the insert in this plasmid was determined by the dideoxy chain determination method using T7 DNA polymerase (Pharmacia LKB Biotechnology, Inc.) and oligonucleotides synthesized according to flanking plasmid sequences and subsequently according to the insert sequence. This sequence data was used to generate additional synthetic deoxynucleotides which were then used to sequence portions of the insert in pFT-3. Sequence analysis was performed using the sequence analysis software package of the University of Wisconsin Genetics Computer Group.

Southern Blot Analysis

High molecular weight human genomic DNA was prepared from whole peripheral blood. Genomic DNA (10 μg) was digested with restriction endonucleases, fractionated through a 0.8% agarose gel, and subjected to Southern blot analysis. To aid in the comparison of hybridization patterns obtained with different probes, duplicate blots were prepared from identical sets of restriction digests electrophoresed on a single gel. Southern blots were hybridized with the temperature being maintained at 35° C. Southern blots were probed with the 1.7 Kb XhoI-XbaI fragment of plasmid pCDM7-α(1,3/1,4)FT which represents the 5' end of the human cDNA encoding the Lewis α(1,3/1,4)fucosyltransferase enzyme. Alternatively, Southern blots were probed with a 400 bp AvaII-PvuII fragment isolated from the insert in pFT-3. Following hybridization, blots were rinsed twice in 2X SSC 0.5% SDS at room temperature for 10 minutes, washed, and then subjected to autoradiography.

Northern Blot Analysis

Total RNA was prepared from cultured cells Poly A+ RNA was then isolated from total RNA by p;ogp dT cellulose column chromatography using commercially supplied columns (Clontech) and procedures supplied by the manufacturer. RNA samples were electrophoresed through 1.0% agarose gels containing formaldehyde and were then transferred to a nylon tembrane (Hybond-N, Amersham). Northern blots were prehybridized for 1 hour at 61° C. in 1X PE (16), 5X SSC, 1% sodium dodecyl sulfate, and 100 μg/ml sheared salmon sperm DNA. Blots were then hybridized for at least 16 hours at 61° C. in the same hybridization solution. The probe was a radiolabelled 400 bp AvaII-PvulI fragment isolated from the insert in pFT-3. Following hybridization, blots were subjected to three, ten minute room temperature rinses in 2X SSC, and then washed for 30 minutes at 62° C. in 2X SSC, -0.2% SDS.

Transfection and Expression of the Insert in PFT-3

The 3.8 Kb PstI insert in plasmid pFT-3 was excised and cloned into the PstI site in the mammalian expression plasmid pCDNA1 (Invitrogen). One plasmid with a single insert in the sense orientation with respect to the plasmid's CMV promoter enhancer sequences was designated pCDNA1-Fuc-TIV, and was used for subsequent analysis.

Construction and radiolabeling of stably transfected CHO cell lines

CHO Ade-C cells were transfected with ScaI-linearized pCDM7-Fuc-TIV, co-precipitated in a 10-fold molar excess over EcoRI-linearized pSV2-Neo. A single, clonal, SSEA-1-positive cell line (CHO-FT3) was derived from this population. Cell extracts prepared from CHO-FT3 contained substantial amounts of α(1,3)fucosyltransferase activity when assayed with the acceptor N-acetyllactosamine.

FACS Analysis

COS-1 cells transfected with plasmid DNAs were harvested 48-72 hours after transfection, and stained with monoclonal antibodies diluted in staining media. Anti-Lewis a and anti-H antibodies (mouse IgM monoclonal; antigen-affinity purified; Chembiomed, Edmonton) were used at 10 μg/ml. Anti-SSEA-1 (mouse monoclonal IgM; ascites) was used at a dilution of 1:1000. Anti-sialyl Lewis x (mouse monoclonal IgM; HPLC purified from ascites) was used at 10 μg/ml. Anti-sialyl Lewis a (mouse monoclonal IgG3; ammonium sulfate precipitate of ascites) was used at a dilution of 1:1000. Control mouse IgG3 antibody (MsIg, Coulter) was used at a concentration of 10 μg/ml. Anti-VIM-2 antibody (mouse monoclonal IgM; ascites) was used at a dilution of 1:200. Cells were then stained with fluorescein isothiocyanate-conjugated goat anti-mouse IgM or IgG, as appropriate, and were then subjected to analysis on a FACScan (Becton-Dickinson).

Fucosyltransferase Assays

Cell extracts containing 1% Triton X-100 were prepared from transfected COS-1 cells. Fucosyltransferase assays were performed in a total volume of 20 μl, and contained 50 mM sodium cacodylate, pH 6.2, 5 mM ATP, 10 mM fucose, 20 mM MnCl₂, 3 μM GDP-¹⁴ C-fucose, and 5 μl (30 μg protein) of cell extract. Acceptor substrates were added to a final concentration of 20 mM. Reactions were incubated at 37° C. for 1 hour and terminated by addition of 20 μl ethanol, followed by addition of 600 μl of distilled water. An aliquot of each reaction (50 μl) was subjected to scintillation counting to determine total radioactivity in the reaction. Another aliquot (200 μl) was applied to a column containing 400 μl of Dowex IX2-400, formate form. The flow through fraction, and 2 μl of a subsequent water elution, were collected and pooled, and an aliquot was subjected to scintillation counting to quantitate incorporation of radioactive fucose into neutral product. Descending paper chromatography was used to confirm the structure of the product formed with the acceptor N-acetyllactosamine. The neutral product in the Dowex column eluate was concentrated by lyophilization, resuspended in a small volume of water, and fractionated through Whatman No. 40 in phenol/isopropanol/formic acid/water (85:5:10:100, lower layer). After chromatography (40 hours), the air-dried chromatogram was cut into 1 cm strips and the strips eluted into 2 ml of water. Radioactivity in each eluate was determined by scintillation counting after mixing with 10 ml of scintillation cocktail.

An affinity-purified, protein A-Lewis fucosyltransferase fusion protein was used to prepare an authentic, radiolabeled Galβ(1→4) ¹⁴ C-Fucα(1→3)!GlcNAc standard for this analysis. This fusion protein was incubated with 20 mM N-acetyllactosamine, 150 μm GDP- (¹⁴ C!fucose (sp. act.=3,800 cpms/nmol), in a standard fucosyltransferase reaction mixture. The neutral, radiolabeled product was purified by amine adsorption HPLC on a Waters Carbohydrate Analysis column, using an isocratic gradient consisting of 70% acetonitrile in water, at a flow rate of 1 ml/min. The product (17 nmol) was subjected to analysis by ¹⁴ C NMR (Center for Complex Carbohydrate Research, Athens, Ga.). The sample was exchanged repeatedly in D₂ O and subjected to NMR analysis at 500 MHz. The proton NMR spectrum was recorded on a Bruker AM500 instrument at 28° C. Chemical shifts are relative to acetate (δ1.908 ppm). The structure of the expected trisaccharide Galβ(1→4) Fucα(1→3))GlcNAc, was verified by 500 MHz spectroscopy. The spectrum, recorded in D₂ O at 28° C., showed H-1 signals for GlcNAC (α-anomer) at δ≈5.102, for Gal at δ≈4.467 and 4.454 ppm (for the α- and β-anomers, respectively, of the trisaccharide) and for Fuc at δ≈5.102 ppm. The anomeric signal for the β-anomer of GlcNAc was obscured by the residual HOD peak (δ≈4.72 ppm). The methyl signals of the GlcNAc N-acetyl group and the C6 protons of Fuc were observed at δ≈2.032 and 1.178 ppm, respectively. These chemical shifts match those published for the authentic trisaccharide Galβ(1→4)(Fucα(1→3)!GlcNAc.

α-L-Fucosidase Digestion

The neutral, chromatographically-purified radiolabeled fucosyltransferase product was subjected to a-L-fucosidase digestion to confirm the alpha anomeric configuration of the attached fucose. 3- ¹⁴ C! fucosyl-N-acetyllactosamine was purified by descending paper chromatography as described above, and an aliquot (7000 cpms) was digested with 40 mU of α-L-fucosidase (E.C. 3.2.1.51, Boehringer-Mannheim) in 20 μl of 100 mM Na citrate pH 5.5, at 37° C. for 22 hrs. The reaction was desalted by Dowex column chromatography and subjected to HPLC analysis using conditions described above for preparation of the radiolabeled standard. The product of the digestion was identified by comparison to parallel separations of L- ¹⁴ C! fucose and the 3- ¹⁴ C!fucosyl-N-acetyllactosamine starting material. Quantitative release of L- ¹⁴ C!fucose was achieved by α-L-fucosidase digestion.

Example V

Isolation of a GDP-Fuc:β-D-Gal(1,4)-D-GlcNAc α(1,3)Fucosvltransferase (Fuc-TV. DNA SEQ ID NO:10, Protein SEQ ID NO:11) through cross-hybridization

Molecular cloning of a human genomic DNA segment that cross-hybridizes to the Lewis blood group α(1,3/1,4) fucosyl-transferase cDNA: Low stringency Southern blot hybridization experiments have indicated to the inventor that the coding region of the Lewis fucosyltransferase cDNA detects strongly hybridizing restriction fragments, as well as several weakly hybridizing fragments.

The inventor expected that the strongly hybridizing fragments represented one or more genes similar to the Lewis fucosyltransferase cDNA. To further examine the molecular nature of these sequences, as noted above in Example IV, the inventor screened a human lambda phage genomic DNA library at low stringency with the Lewis cDNA probe. A total of 18 phages were isolated from phages representing approximately five human genomic equivalents. Southern blot analysis of 16 of these phages allowed them to be placed into three groups, based upon their restriction patterns and hybridization signal intensity strengths. Several phages representing a class with strong hybridization intensities were identified. Cross-hybridizing restriction fragments isolated from one of these phages was subcloned and sequenced.

The homologous DNA restriction fragment maintains a single long open reading frame that predicts a polypeptide with strong similarity to the Lewis blood group α(1,3/1,4) fucosyltransferase cDNA: DNA sequence analysis of the subcloned fragments identified a single long open reading frame within its 3' portion, beginning at base pair 1 and ending at base pair 1125 (SEQ ID NO:10) (see FIG. 6). This reading frame begins with a methionine codon that is found within a sequence context consistent with Kozak's consensus rules for mammalian translation initiation. A hydropathy analysis of the protein sequence predicted by this reading frame (SEQ ID NO:11) would predict a single hydrophobic segment at its NH₂ -terminus, suggesting that the predicted polypeptide would maintain the type II transmembrane orientation typical of mammalian glycosyltransferases. Virtually the entire length of this reading frame shares a strikingly high amount of amino acid and nucleic acid sequence identity with the corresponding portion of the Lewis fucosyltransferase cDNA (FIG. 6). This sequence similarity diverges in just a few positions, most notably at base pair 139 within the open reading frame. This 33 base pair insertion, relative to the Lewis cDNA, would create a peptide insertion of 11 amino acids, relative to the Lewis fucosyltransferase. Because of the substantial sequence similarity between these two DNA sequences, and their derived protein sequences, the inventor expected this new cross-hybridizing sequence to represent a single exonic sequence, representing a heretofore undefined gene, that encodes a fucosyltransferase.

The homologous DNA restriction fragment determines expression of an α(1,3)fucosyltransferase: To determine if this segment encodes a functional fucosyltransferase, a 1.94 kb Earl-Xbal fragment containing the entire open reading frame was cloned into a mammalian expression vector, and the resulting plasmid (pCDNA1-Fuc-TV) was introduced into mammalian host cells by transfection. COS-1 cells were used as hosts for these experiments since these cells normally express virtually undetectable α(1,3)- and α(1,4)fucosyltransferase activities. Likewise, COS-1 cells do not normally express detectable amounts of cell surface Galβ1→4(Fucα(1→3))GlcNAc- (Lewis x, SSEA-1) or Galβ1→3 Fucα(1→4)!GlcNAc- (Lewis a) moieties, whereas they do maintain surface-display of non-fucosylated type II and type I oligosaccharide precursors necessary for the construction of such molecules. The vector pCDNAI was used, since this plasmid efficiently transcribes exogenous, subcloned sequences in COS-1 hosts by virtue of the cytomegalovirus immediate early promoter sequences in the vector, and is maintained in these cells as a multicopy episome.

COS-1 cells transfected with pCDNA1-Fuc-TV were first analyzed by flow cytometry to detect de novo, vector-dependent surface expression of these oligosaccharides. A substantial fraction of these transfected cells exhibited bright staining with a monoclonal antibody directed against the Lewis x moiety (Galβ(1→4) Fucα(1→3)!GlcNAc-) (FIG. 8), whereas cells transfected with the pCDNA1 vector without insert did not express this determinant. By contrast, COS-1 cells transfected with pCDNA1-Fuc-TV, or with its control plasmid, did not stain with antibodies specific for the type I-based Lewis a trisaccharide (FIG. 8).

Taken together, these results are consistent with the results of DNA sequence analysis indicating that this segment encodes an α(1,3)fucosyltransferase competent to utilize neutral type II oligosaccharide precursor, but that the enzyme cannot efficiently utilize type I glycoconjugates and thus does not exhibit strong α(1,4)fucosyltransferase activity.

There is evidence supporting the possibility that one or more human α(1,3)fucosyltransferases exist that can utilize type II acceptors whose terminal galactose residues are substituted with α(2,3)sialic acid moieties. Such enzymes can fucosylate these molecules to form the sialyl-Lewis x determinant (NeuAcα(2→3)Galβ(1→4) Fucα(1→3)!GlcNAc-). The Lewis fucosyltransferase, for example, is competent to perform this reaction.

It was therefore of interest to determine if the fucosyltransferase apparently encoded by plasmid pCDNA1-Fuc-TV would be capable of constructing sialyl-Lewis X determinants. COS-1 cells maintain surface-expressed glycoconjugates terminating in (NeuAcα(2,3)Galβ(1,4)GlcNAC-; these represent acceptor substrates for sialyl-Lewis construction determined via the action of enzymes encoded by transfected fucosyltransferase expression vectors. COS-1 cells were therefore transfected with plasmid pCDNA1-Fuc-TV, stained with a monoclonal anti-sialyl Lewis x antibody, and subjected to flow cytometry analysis. A significant amount of staining was detected, relative to control cell transfected with the vector alone, or relative to pCDNA1-α(1,3)-Fuc-TV-transfected cells stained with a negative control antibody (anti-H). (FIG. 8).

However, these cells did not stain with an antibody specific for the type I-based, sialyl-Lewis a determinant (NeuAcα(2,3)Galβ(1,3) Fucα(1,4)!GlcNAc-) (FIG. 8). By contrast, the inventor had previously observed that a substantial fraction of COS-1 cells transfected with the Lewis fucosyltransferase expression plasmid pCDM7-α(1,3/1,4)FT exhibit bright staining with the sialyl-Lewis x and sialyl-Lewis a antibodies, as predicted by biochemical analysis of the acceptor substrate specificity of this enzyme.

The conclusion that plasmid pCDNA1-Fuc-TV encodes an α(1,3)fucosyltransferase was confirmed by biochemical analysis of the acceptor substrate requirements of the enzyme in extracts of COS-1 cells transfected with plasmid pCDNA1-Fuc-TV. As expected, the enzyme in these extracts utilized the type II disaccharide acceptor N-acetyllactosmine, to yield the predicted product Galβ(1→4) Fucα(1→3)!GlcNAc. The enzyme also efficiently utilized the trisaccharide NeuAcα(2,3)Galβ(1,4)GlcNac to form the sialyl Lewis x tetrasaccharide (Table 2). Under these conditions, other type II molecules, including lactose, and the α(1,2)fucosylated type II acceptor Fucα(1→2)Galβ(1→4)Glc, did function as acceptor substrates for the fucosyltransferase in these extracts, although with substantially lower efficiencies. (Summarized in Table 2).

Interestingly, the type I substrate lacto-N-biose I is also utilized by this enzyme, although again at low efficiency (Table 2). This suggests that the enzyme can also function as an α(1,4)fucosyltransferase, but at a very low efficiency, as also suggested by the absence of α(1,4) structures on flow cytometry analysis. The acceptor preferences exhibited by this enzyme contrast with that exhibited by the Lewis fucosyltransferase, which is able to efficiently utilize each of the four acceptors tested.

To further demonstrate that enzymatic activity is directly associated with this protein, the putative catalytic domain of the predicted polypeptide (amino acids 43 to 374 of SEQ ID NO: 11) was fused to a secreted form of the IgG binding domain of Staphylococcus aureus protein A in the mammalian expression vector pPROTA, to generate the vector pPROTA-Fuc-TV. Since this fusion protein would lack the putative transmembrane anchoring segment of the fucosyltransferase, the inventor expected it would be synthesized as a secreted molecule that could be affinity-purified on an IgG-containinq matrix and subsequently tested for α(1,3)fucosyltransferase activity. COS-1 cells transfected with the control vector pCDM7 or pPROTA generated no detectable cell-associated or released enzyme activity. However, conditioned media prepared from COS-1 cells transfected with pPROTA-Fuc-TV, contained significant quantities of α(1,3)fucosyltransferase activity when assayed with N-acetyllactosmine. Virtually 100% of the released activity generated by pPROTA-Fuc-TV, is specifically retained by the IgG-Sepharose matrix. These results indicate that the protein encoded by this cloned DNA segment encodes a fucosyltransferase, and demonstrate that information sufficient to generate α(1,3)fucosyltransferase activity resides within the enzyme's COOH-terminal 332 amino acids.

Taken together with the results of the flow cytometry analyses and DNA sequence analysis, these experiments indicate that plasmid pCDNA1-Fuc-TV encodes a novel α(1,3)fucosyltransferase.

Experimental Procedures for Example V, "Isolation of a GDP-Fuc-β-D-Gal (1,4)-D-GlcNAc α(1 3)Fucosyltransfedrase (Fuc-TV) through cross-hybridization

Cell Culture

The source and growth conditions of COS-1 cells used are as previously described. See Ernst et al;, J. Biol. Chem. (1989) 265:3436-3447 and Rajan et al. J. Biol. Chem. (1989) 264:11158-11167.

Antibodies

The anti-Lex antibody anti-SSEA-1 (mouse monoclonal IgM as ascites) was used. Solter et al, Proc. Nat. Acad. Sci. (USA) (1978), 75:5565-5569. Anti-H and anti-Lewis a antibodies (mouse monoclonal IgM, antigen affinity purified) were purchased from Chembiomed Ltd. (Edmonton, Alberta). Anti-sialyl Lewis x antibody CSLEX1 (Fukushima et al, Cancer Res. (1984) 44:5279-5285) (mouse monoclonal IgM, HPLC purified) and anti-sialyl Lewis a antibody CSLEA1 (Chia et al, Cancer Res. (1985) 45:435-437) (mouse monoclonal IgG3, ammonium sulfate precipitate) were used. Fluorescein-conjugated goat anti-mouse IgM or IgG antibodies were purchased from Sigma.

Human Genomic Library Construction

High molecular weight human genomic DNA was prepared from peripheral blood leukocytes. Genomic DNA was subjected to partial digestion with the restriction endonuclease Sau3A. The partially digested genomic DNA was size fractionated by ultracentrifugation through a sodium chloride gradient. Fractions enriched for DNA fragments between 8 Kb and 20 Kb were ligated to Xhol digested lambda FIX (Stratagene) phage arms that had been partially filled in with dTTP and dCTP to make the ends compatible with the Sau3A fragments.

The ligation mixture was packaged in vitro with commercial packaging extracts (Stratagene), tittered on E. coli host TAP90. Approximately 1.0×10⁶ recombinant lambda phage were screened by plaque hybridization. Plaque lifts were prepared using nitrocellulose filters (Schleicher and Schuell) and were prehybridized at 42° C. for 16 hours in 50% formamide, 5X SSC, 10X Denhart's solution, and 0.1% SDS. Filters were hybridized for 72 hours at 35° C. in prehybridization solution containing 10% dextran sulfate, and 100 micrograms per ml denatured salmon sperm DNA. The probe consisted of a 1.7 Kb Xhol-Xbal fragment isolated from the 5' end of a cDNA insert encoding the Lewis blood group α(1,3/1,4) fucosyltransferase, which was labeled with α-³² P! dCTP. The filters were rinsed three times for 20 minutes each at room temperature in 2X SSC and then once for 40 minutes at 50° C. and 1X SSC, 0.5% SDS. Filters were then subjected to autoradiography. Eighteen independent hybridization-positive plaques were identified after 2 additional cycles of plaque hybridization. Phage DNAs were prepared from liquid lysates and were subsequently characterized by restriction endonuclease digestions and Southern blot analyses.

DNA Sequence Analysis

Phage DNA was digested with various restriction enzymes, and fragments homologous to the human α(1,3/1,4)fucosyltransferase cDNA were gel purified and ligated into the multicloning site of pTZ18R. Representative subclones were sequenced by the dideoxy chain determination method using T7 DNA polymerase (Pharmacia LKB Biotechnology, Inc.) and oligonucleotides synthesized according to flanking plasmid sequences and subsequently according to the insert sequence. This sequence data was used to generate additional synthetic deoxynucleotides which were then used to sequence remaining portions of the inserts. Sequence analysis was performed using the sequence analysis software package of the University of Wisconsin Genetics Computer Group.

Construction of Plasmid pCDNA1-Fuc-TV

A 1.94 kb Earl-Xbal fragment was isolated from a representative phage taken from a strongly hybridizing class of phages, made blunt with the Klenow fragment of E. coli DNA polymerase I, and cloned into the EcoRV and Xbal sites in the mammalian expression plasmid pCDNA1 (Invitrogen). One plasmid with a single insert in the sense orientation with respect to the plasmid's CMV promoter enhancer sequences was designated pCDNA1-Fuc-TV.

FACS Analysis

COS-1 cells transfected with plasmid DNAs were harvested 48-72 hours after transfection, and stained with monoclonal antibodies diluted in staining media. Anti-Lewis a and anti-H antibodies (mouse IgM monoclonal; antigen-affinity purified; Chembiomed, Edmonton) were used at 10 μg/ml. Anti-SSEA-1 (mouse monoclonal IgM; ascites) was used at a dilution of 1:1000. Anti-sialyl-Lewis x (mouse monoclonal IgM; HPLC purified from ascites) was used at 10 μg/ml. Anti-sialyl Lewis a (mouse monoclonal IgG3; ammonium sulfate precipitate of ascites) was used at a dilution of 1:500. Cells were then stained with fluorescein isothiocyanate-conjugated goat anti-mouse IgM or IgG, as appropriate, and were then subjected to analysis on a FACScan (Becton-Dickinson).

Fucosyltransferase Assays

Cell extracts containing 1% Triton X-100 were prepared from transfected COS-1 cells. Fucosyltransferase assays were performed in a total volume of 20 μl, and contained 25 mM sodium cacodylate, pH6.2, 5 mM ATP, 10 mM L-fucose, 10 mM MnCl₂, 3 μM GDP-¹⁴ C-fucose, and 5 μl of cell extract. Acceptor substrates were added to a final concentration of 20 mM. Reactions were incubated at 37° C. for 1 hour and terminated by addition of 20 μl ethanol, followed by addition of 600 μl of distilled water. An aliquot of each reaction (50 μl) was subjected to scintillation counting to determine total radioactivity in the reaction. Another aliquot (200 μl) was applied to a column containing 400 μl of Dowex 1X2-400, formate form. The flow through fraction, and 2 μl of a subsequent water elution, were collected and pooled, and an aliquot was subjected to scintillation counting to quantitate incorporation of radioactive fucose into neutral product.

Example VI

Cloning and expression of a DNA sequence encoding a GDP-Fuc:β-D-Gal(1,4)-D-GlcNAc α(1,3)fucosyltransferase" (Fuc-TVI:DNA SEQ ID NO:13, protein SEQ ID NO:14) through cross hybridization.

Biochemical and genetic studies indicate that the human genome encodes two or more distinct GDP-L-fucose:β-D-Galactoside 3-α-L-Fucosyltransferases (Potvin et al, J. Biol. Chem., 265:1615-1622, 1990; Watkins, Adv. Hum. Genet., 10:1-116, 1980). the inventor has recently described a cloned cDNA that encodes one of these enzymes, that is thought to represent the product of the human Lewis blood group locus (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990) (DNA SEQ ID NO:1 and Protein SEQ ID NO:2). In consideration of the possibility that these GDP-L-fucose:β-D-Galactoside 3-α-L-Fucosyltransferases might be encoded by a family of structurally-related genes, the inventor sough to isolate other such members by cross-hybridization methods, using the cloned Lewis fucosyltransferase cDNA.

Molecular cloning of a human genomic DNA sectment that crosshybridizes to the Lewis blood group α(1,3/1,4)fucosyltransferase cDNA As noted above in Examples IV and V, low stringency Southern blot hybridization experiments indicate that the coding region of the Lewis fucosyltransferase cDNA detects strongly hybridizing restriction fragments, as well as several weakly hybridizing fragments. To further examine the molecular nature of these sequences, the inventor screened a human lambda phage genomic DNA library at low stringency with the Lewis cDNA probe. A total of 18 phages were isolated from phages representing approximately five human genomic equivalents. Southern blot analysis of 16 of these phages allowed them to be placed into three groups, based upon their restriction patterns and hybridization signal intensity strengths. Several phages representing a class with strong hybridization intensities were identified. Cross-hybridizing restriction fragments isolated from one of these phages was subcloned and sequenced.

The homologous DNA restriction fragment maintains a single long open reading frame that predicts a polypentide with strong similarity to the Lewis blood group α(1,3/1,4)fucosyltransferase cDNA

DNA sequence analysis of the cross-hybridizing subcloned fragment (SEQ ID NO:13) identified a single long open reading frame, beginning at base pair 1 and ending at base pair 1080 (FIG. 7). This reading frame begins with a methionine codon that is found within a sequence context consistent with Kozak's consensus rules for mammalian translation initiation. A hydropathy analysis of the protein sequence predicted by this reading frame (SEQ ID NO:14) predicts a single hydrophobic segment at its NH₂ -terminus, suggesting that it represents a 359 amino acid protein (SEQ ID NO:14) that is predicted to maintain the type II transmembrane orientation typical of mammalian glycosyltransferases. Virtually the entire length of this reading frame shares a strikingly high amount of amino acid (not shown) and nucleic acid sequence identity with the corresponding portion of the Lewis fucosyltransferase cDNA (FIG. 7). Because of the substantial sequence similarity between these two DNA sequences, and their derived protein sequences, we expected this new cross-hybridizing sequence to represent a single exonic sequence, representing a heretofore undefined gene, that encodes a fucosyltransferase.

The homologous DNA restriction fragment determines expression of an α(1,3)fucosyltransferase

To determine if this segment encodes a functional fucosyltransferase, a 1.2 kb fragment containing the entire open reading frame was generated by the polymerase chain reaction and was cloned into a mammalian expression vector, and the resulting plasmid (pCDNA1-Fuc-TVI) was introduced into mammalian host cells by transfection. COS-1 cells were used as hosts for these experiments since these cells normally express virtually undetectable α(1,3)- and α(1,4)fucosyltransferase activities (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990). Likewise, COS-1 cells do not normally express detectable amounts of cell surface Galβ1→4 Fucα(1→3)!GlcNAc-(Lewis x, SSEA-1) or Galβ1→3 Fucα(1→4)!GlcNAc-(Lewis a) moieties, whereas they do maintain surface-display of non-fucosylated type II and type I oligosaccharide precursors necessary for the construction of such molecules (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990). The vector pCDNA1 was used since this plasmid efficiently transcribes exogenous, subcloned sequences in COS-1 hosts by virtue of the cytomegalovirus immediate early promoter sequences in the vector, and is maintained in these cells as a multicopy episome (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990). COS-1 cells transfected with pCDNA1-Fuc-TVI were first analyzed by flow cytometry to detect de novo, vector-dependent surface expression of these oligosaccharides. A substantial fraction of these transfected cells exhibited bright staining with a monocional antibody directed against the Lewis x moiety (Galβ(1→4) Fucα(1→3)!GlcNAc-) (FIG. 8), whereas cells transfected with the pCDNA1 vector without insert did not express this determinant. By contrast, COS-1 cells transfected with pCDNA1-Fuc-TVI, or with its control plasmid, did not stain with antibodies specific for the type I-based Lewis a trisaccharide (FIG. 8). Taken together, these results are consistent with the results of the DNA sequence analysis indicating that this segment encodes an α(1,3)fucosyltransferase competent to utilize neutral type II oligosaccharide precursor, but that the enzyme cannot utilize type I glycoconjugates and thus does not exhibit α(1,4)fucosyltransferase activity.

There is evidence supporting the possibility that one or more human α(1,3)fucosyltransferases exist that can utilize type II acceptors whose terminal galactose residues are substituted with α(2,3)sialic acid moieties (Potvin et-al, J. Biol. Chem., 265:1615-1622, 1990; Holmes et al, J. Biol. Chem., 261:3737-3743, 1986; Palcic et al, Carbohyd. Res., 190:1-11, 1989). Such enzymes can fucosylate these molecules to form the sialyl-Lewis x determinant (NeuAcα(2→3)Galβ(1→4) Fucα(1→3)!GlcNAc-). The Lewis fucosyltransferase, for example, is competent to perform this reaction (Palcic et al, Carbohyd. Res., 190:1-11, 1989; Lowe et al, Cell, 63:475-484, 1990). It was therefore of interest to determine if the fucosyltransferase apparently encoded by plasmid pCDNA1-α(1,3)Fuc-TVI would be capable of constructing sialyl-Lewis x determinants. COS-1 cells maintain surface-expressed glycoconjugates terminating in (NeuAcα(2,3)Galβ(1,4)GlcNAc- (Lowe et al, Cell, 63:475-484, 1990; Fukuda et al, J. Biol. Chem., 263:5314-5318, 1988); these represent acceptor substrates for sialyl-Lewis x construction determined via the action of enzymes encoded by transfected fucosyltransferase expression vectors (Lowe et al, Cell, 63:475-484, 1990). COS-1 cells were therefore transfected with plasmid pCDNA1-Fuc-TVI, stained with a monoclonal anti-sialyl Lewis x antibody, and subjected to flow cytometry analysis. A significant amount of staining was detected, relative to control cell transfected with the vector alone, or relative to pCDNA1-Fuc-TVI-transfected cells stained with a negative control antibody (anti-H, FIG. 8). However, these cells did not stain with an antibody specific for the type I-based, sialyl-Lewis a determinant (NeuAcα(2,3)Galβ(1,3) Fucα(1,4)!GlcNAc-) (FIG. 8). By contrast, the inventor has previously demonstrated that a substantial fraction of COS-1 cells transfected with the Lewis fucosyltransferase expression plasmid pCDM7-α(1,3/1,4)FT exhibit bright staining with the sialyl-Lewis x and sialyl-Lewis a antibodies (Lowe et al, Cell, 63:475-484, 1990), as predicted by biochemical analysis of the acceptor substrate specificity of this enzyme (Palcic et al, Carbohyd. Res., 190:1-11, 1989).

The conclusion that plasmid pCDNA1-Fuc-TVI encodes an α(1,3)fucosyltransferase was confirmed by biochemical analysis of the acceptor substrate requirements of the enzyme in extracts of COS-1 cells transfected with plasmid pCDNA1-Fuc-TVI. As expected, the enzyme in these extracts utilized the type II disaccharide acceptor N-acetyllactosmine, to yield the predicted product Galβ(1→4) Fucα(1→3)!GlcNAc. The enzyme also efficeintly utilized the trisaccharide NeuAcα(2,3)Galβ(1,4)GlcNAc to form the sialyl Lewis X tetrasaccharide (Table 2). Under these conditions, other type II molecules, including lactose, and the α(1,2)fucosylated type II acceptor Fucα(1→2)Galβ(1→4)Glc, did not function as acceptor substrates for the fucosyltransferase in these extracts, with any detectable efficiency. The type I substrate lacto-N-biose I was also not utilized by this enzyme. This suggests that the enzyme can function effectively only as an α(1,3)fucosyltransferase, as also suggested by the flow cytometry analyses. The acceptor preferences exhibited by this enzyme contrast significantly with those exhibited by the Lewis fucosyltransferase, which is able to efficiently utilize each of the four acceptors tested (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990; Mollicone et al, Eur. J. Biochem., 191:169-176, 1990). Taken together with the results of the flow cytometry analyses presented in FIG. 8, and DNA sequence analysis, these biochemical experiments, summarized in Table 2, indicate that plasmid pCDNA1-Fuc-TVI encodes a novel fucosyltransferase with its own distinct acceptor specificity.

Since the protein sequence of Fuc-TVI is so very similar to the sequences of FUC-TV and FUC-TIII, it may be expected that a catalytically active, secreted protein A-Fuc-TVI fusion porotein may be generated by fusing residues 43 through 359 of Fuc-TVI (SEQ ID NO:14) to the protein A segment, in a manner identicial to that used to generate pPROTA-α(1,3/1,4)FT:, and pPROTA-Fuc-TV_(c).

Experimental Procedures for Example VI "Cloning and expression of a DNA sequence encoding GDP-Fuc-β-D-Gal(1,4)-D-GlcNAc α(1,3) fucosyltransferasel" (Fuc-TVI; DNA SEQ ID NO:14. protein SEQ ID NO:15) through cross hybridation

Cell Culture

The source and growth conditions of COS-1 cells are as previously described (Ernst et al, J. Biol. Chem., 265:3436-3447, 1989; Rajan et al, J. Biol. Chem., 264:11158-11167, 1989).

Antibodies

The anti-Lex antibody anti-SSEA-1 (Solter et al, Proc. Natl. Acad. Sci. USA, 75:5565-5569, 1978) (mouse monoclonal IgM as ascites) was provided by Dr. Davor Solter (Wistar Institute, Philadelphia). Anti-H and anti-Lewis a antibodies (mouse monoclonal IgM, antigen affinity purified) were purchased from Chembiomed Ltd. (Edmonton, Alberta). Anti-sialyl Lewis x antibody CSLEX1 (Fukushima et al, Cancer Res., 44:5279-5285, 1984) (mouse monoclonal IgM, HPLC purified) and anti-sialyl Lewis a antibody CSLEA1 (Galton et al, Ninth Int. Convoc. Immuno., Amherst, N.Y., pp. 117-125, Karger, Basel; Chia et al, Cancer Res., 45:435-437, 1985) (mouse monoclonal IgG3, ammonium sulfate precipitate) were provided by Dr. P. Terasaki (UCLA, Los Angeles). A pooled mouse IgG antibody preparation (MsIg) was purchased from Coulter. Fluorescein-conjugated goat anti-mouse IgM or IgG antibodies were purchased from Sigma.

Human Genomic Library Construction

High molecular weight human genomic DNA was prepared from peripheral blood leukocytes as described previously (Ernst et al, J. Biol. Chem., 265:3436-3447, 1989). Genomic DNA was subjected to partial digestion with the restriction endonuclease Sau3A. The partially digested genomic DNA was size fractionated by ultracentrifugation through a sodium chloride gradient. Fractions enriched for DNA fragments between 8 Kb and 20 Kb were ligated to XhoI digested lambda FIX (Stratagene) phage arms that had been partially filled in with dTTP and dCTP to make the ends compatible with the Sau3A fragments. The ligation mixture was packaged in vitro with commercial packaging extracts (Stratagene), titered on E. coli host TAP90 (Patterson et al, Nucl. Acids. Res., 15:6298, 1987). Approximately 1.0×10⁶ recombinant lambda phage were screened by plaque hybridization. Plaque lifts were prepared using nitrocellulose filters (Schleicher and Schuell) and were prehybridized at 42° C. for 16 hours in 50% formamide, 5X SSC, 10X Denhart's solution, and 0.1% SDS. Filters were hybridized for 72 hours at 35° C. in prehybridization solution containing 10% dextran sulfate, and 100 micrograms per ml denatured salmon sperm DNA. The probe consisted of a 1.7 Kb XhoI-XbaI fragment isolated from the 5' end of a cDNA insert encoding the Lewis blood group α(1,3/1,4) fucosyltransferase (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990), which was labeled (Feinberg et al, Anal. Biochem., 132:6-13, 1983) with α-³² P! dCTP. The filters were rinsed three times for 20 minutes each at room temperature in 2X SSC and then once for 40 minutes at 50° C. and 1X SSC, 0.5% SDS. Filters were then subjected to autoradiography. Eighteen independent hybridization-positive plaques were identified after 2 additional cycles of plaque hybridization. Phage DNAs were prepared from liquid lysates (Maniatis et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Lab., Cold Spring Harbor, N.Y., 1982) and were subsequently characterized by restriction endonuclease digestions and Southern blot analyses.

DNA Sequence Analysis

Phage DNA was digested with various restriction enzymes, and fragments homologous to the human α(1,3/1,4)fucosyltransferase cDNA were gel purified and ligated into the multicloning site of pTZ18. Representative subclones were sequenced by the dideoxy chain determination method (Sanger et al, Proc. Natl. Acad. Sci. USA, 74:5463-5467, 1977) using T7 DNA polymerase (Pharmacia LKB Biotechnology, Inc.) and oligonucleotides synthesized according to flanking plasmid sequences and subsequently according to the insert sequence. This sequence data was used to generate additional synthetic deoxynucleotides which were then used to sequence remaining portions of the inserts. Sequence analysis was performed using the sequence analysis software package of the University of Wisconsin Genetics Computer Group (Devereux et al, Nucl. Acids. Res., 12:387-395, 1984).

Transfection and expression of the insert in pCDNA1-Fuc-TVI

A 1.2 kb fragment was generated by the PCR, using DNA isolated from a representative phage taken from a strongly hybridizing class of phages, and cloned into the HindIII site in the mammalian expression plasmid pCDNA1 (Invitrogen). One plasmid with a single insert in the sense orientation with respect to the plasmid's CMV prmnoter enhancer sequences was designated pCDNA1-Fuc-TVI.

FACS Analysis

COS-1 cells transfected with plasmid DNAs were harvested (Rajan et al, J. Biol. Chem., 264:11158-11167, 1989) 48-72 hours after transfection, and stained with monoclonal antibodies diluted in staining media, as previously described (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990; Ernst et al, J. Biol. Chem., 265:3436-3447, 1989). Anti-Lewis a and anti-H antibodies (mouse IgM monoclonal; antigen-affinity purified; Chembiomed, Edmonton) were used at 10 μg/ml. Anti-SSEA-1 (mouse monoclonal IgM; ascites) was used at a dilution of 1:1000. Anti-sialyl-Lewis x (mouse monoclonal IgM; HPLC purified from ascites) was used at 10 μg/ml. Anti-sialyl lewis a (mouse monoclonal IgG3; ammonium sulfate precipitate of ascites) was used at a dilution of 1:1000. Control mouse IgG3 antibody (MsIg, Coulter) was used at a concentration of 10 μg/ml. Anti-VIM-2 antibody (mouse monoclonal IgM; ascites) was used at a dilution of 1:200. Cells were then stained with fluorescein isothiocyanate-conjugated goat anti-mouse IgM or IgG, as appropriate, and were then subjected to analysis on a FACScan (Becton-Dickinson), as described previously (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990).

Fucosyltransferase Assays

Cell extracts containing 1% Triton X-100 were prepared from transfected COS-1 cells, using procedures described previously (Kukowska-Latallo et al, Genes Devel., 4:1288-1303, 1990). Fucosyltransferase assays were performed in a total volume of 20 μl, and contained 50 mM sodium cacodylate, pH 6.2, 5 mM ATP, 10 mM fucose, 20 mM MnCl₂, 3 μM GDP-¹⁴ C-fucose, and 5 μl (30 μg protein) of cell extract. Acceptor substrates were added to a final concentration of 20 mM. Reactions were incubated at 37° C. for 1 hour and terminated by addition of 20 μl ethanol, followed by addition of 600 μl of distilled water. An aliquot of each reaction (50 μl) was subjected to scintillation counting to determine total radioactivity in the reaction. Another aliquot (200 μl) was applied to a column containing 400 μl of Dowex 1X2-400, formate form (Rajan et al, J. Biol. Chem., 264:11158-11167, 1989). The flow through fraction, and 2 μl of a subsequent water elution, were collected and pooled, and an aliquot was subjected to scintillation counting to quantitate incorporation of radioactive fucose into neutral product.

                                      TABLE 4     __________________________________________________________________________     Substrate Utilzation Properties of Human α(1,3)Fucosyltransferases                           Relative Activity (%) with                           each α(1,3)Fucosyltransferase     Acceptor substrate                    Product Name                           Fuc-TIII                                Fuc-TIV                                     Fuc-TV                                         Fuc-TVI     __________________________________________________________________________     N-acetyllactosamine (20 mM)                    Lewis x                           100  100  100 100     lactose (20 mM)                    Lewis x                           145  3    11  <1     α(2,3)sialyllactosamine (20 mM)                    sialyl Lewis x                            56  <1   115 110     2'-fucosyllactose (5 mM)                    Lewis y                           254  6    42  <1     lacto-N-biose I (20 mM)                    Lewis a                           420  <1   10  <1     __________________________________________________________________________

Table 2 presents the relative product formation rates obtained with low molecular weight acceptor substrates using cell extracts containing recombinant human α(1,3)fucosyltransferases expressed in transfected COS-1 cells, as described in the preceding sections. Fucosyltransferase assays were performed as described in detail in Lowe et al, J. Biol. Chem., (1991), 266:17467-17477; Weston et al, J. Biol. Chem. (1992), 267:4152-4160; and Kukowska-Latallo et al, Genes. Devel., (1990), 4:1288-1303. For each enzyme, the same extract was used, with saturating amounts of each acceptor oligosaccharide (20 mM, except for 2'-fucosylactose which was used at 5 mM), and GDP- ¹⁴ C!fucose was present at 3 μM. Reaction times and enzyme amounts were adjusted to ensure a linear rate of product formation (less than 15% of the GDP-fucose substrate consumed). Products were separated by column chromatography and quantitated by liquid scintillation counting, and their structures were confirmed by high performance liquid chromatography, as described in Lowe et al, J. Biol. Chem., (1991), 266:17467-17477; Weston et al, J. Biol. Chem. (1992), 267:4152-4160; and Kukowska-Latallo et al, Genes. Devel., (1990), 4:1288-1303.

Obviously, numerous modifications and variations of the present invention are possible in light of the above teachings. It is therefore to be understood that, within the scope of the appended claims, the invention may be practiced otherwise than as specifically described herein.

    __________________________________________________________________________     #             SEQUENCE LISTING     - (1) GENERAL INFORMATION:     -    (iii) NUMBER OF SEQUENCES: 14     - (2) INFORMATION FOR SEQ ID NO:1:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 2043 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: cDNA     -     (iv) ANTI-SENSE: NO     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:     - AGGAAACCTG CCATGGCCTC CTGGTGAGCT GTCCTCATCC ACTGCTCGCT GC - #CTCTCCAG       60     - ATACTCTGAC CCATGGATCC CCTGGGTGCA GCCAAGCCAC AATGGCCATG GC - #GCCGCTGT      120     - CTGGCCGCAC TGCTATTTCA GCTGCTGGTG GCTGTGTGTT TCTTCTCCTA CC - #TGCGTGTG      180     - TCCCGAGACG ATGCCACTGG ATCCCCTAGG GCTCCCAGTG GGTCCTCCCG AC - #AGGACACC      240     - ACTCCCACCC GCCCCACCCT CCTGATCCTG CTATGGACAT GGCCTTTCCA CA - #TCCCTGTG      300     - GCTCTGTCCC GCTGTTCAGA GATGGTGCCC GGCACAGCCG ACTGCCACAT CA - #CTGCCGAC      360     - CGCAAGGTGT ACCCACAGGC AGACACGGTC ATCGTGCACC ACTGGGATAT CA - #TGTCCAAC      420     - CCTAAGTCAC GCCTCCCACC TTCCCCGAGG CCGCAGGGGC AGCGCTGGAT CT - #GGTTCAAC      480     - TTGGAGCCAC CCCCTAACTG CCAGCACCTG GAAGCCCTGG ACAGATACTT CA - #ATCTCACC      540     - ATGTCCTACC GCAGCGACTC CGACATCTTC ACGCCCTACG GCTGGCTGGA GC - #CGTGGTCC      600     - GGCCAGCCTG CCCACCCACC GCTCAACCTC TCGGCCAAGA CCGAGCTGGT GG - #CCTGGGCG      660     - GTGTCCAACT GGAAGCCGGA CTCAGCCAGG GTGCGCTACT ACCAGAGCCT GC - #AGGCTCAT      720     - CTCAAGGTGG ACGTGTACGG ACGCTCCCAC AAGCCCCTGC CCAAGGGGAC CA - #TGATGGAG      780     - ACGCTGTCCC GGTACAAGTT CTACCTGGCC TTCGAGAACT CCTTGCACCC CG - #ACTACATC      840     - ACCGAGAAGC TGTGGAGGAA CGCCCTGGAG GCCTGGGCCG TGCCCGTGGT GC - #TGGGCCCC      900     - AGCAGAAGCA ACTACGAGAG GTTCCTGCCA CCCGACGCCT TCATCCACGT GG - #ACGACTTC      960     - CAGAGCCCCA AGGACCTGGC CCGGTACCTG CAGGAGCTGG ACAAGGACCA CG - #CCCGCTAC     1020     - CTGAGCTACT TTCGCTGGCG GGAGACGCTG CGGCCTCGCT CCTTCAGCTG GG - #CACTGGAT     1080     - TTCTGCAAGG CCTGCTGGAA ACTGCAGCAG GAATCCAGGT ACCAGACGGT GC - #GCAGCATA     1140     - GCGGCTTGGT TCACCTGAGA GGCCGGCATG GTGCCTGGGC TGCCGGGAAC CT - #CATCTGCC     1200     - TGGGGCCTCA CCTGCTGGAG TCCTTTGTGG CCAACCCTCT CTCTTACCTG GG - #ACCTCACA     1260     - CGCTGGGCTT CACGGCTGCC AGGAGCCTCT CCCCTCCAGA AGACTTGCCT GC - #TAGGGACC     1320     - TCGCCTGCTG GGGACCTCGC CTGTTGGGGA CCTCACCTGC TGGGGACCTC AC - #CTGCTGGG     1380     - GACCTTGGCT GCTGGAGGCT GCACCTACTG AGGATGTCGG CGGTCGGGGA CT - #TTACCTGC     1440     - TGGGACCTGC TCCCAGAGAC CTTGCCACAC TGAATCTCAC CTGCTGGGGA CC - #TCACCCTG     1500     - GAGGGCCCTG GGCCCTGGGG AACTGGCTTA CTTGGGGCCC CACCCGGGAG TG - #ATGGTTCT     1560     - GGCTGATTTG TTTGTGATGT TGTTAGCCGC CTGTGAGGGG TGCAGAGAGA TC - #ATCACGGC     1620     - ACGGTTTCCA GATGTAATAC TGCAAGGAAA AATGATGACG TGTCTCCTCA CT - #CTAGAGGG     1680     - GTTGGTCCCA TGGGTTAAGA GCTCACCCCA GGTTCTCACC TCAGGGGTTA AG - #AGCTCAGA     1740     - GTTCAGACAG GTCCAAGTTC AAGCCCAGGA CCACCACTTA TAGGGTACAG GT - #GGGATCGA     1800     - CTGTAAATGA GGACTTCTGG AACATTCCAA ATATTCTGGG GTTGAGGGAA AT - #TGCTGCTG     1860     - TCTACAAAAT GCCAAGGGTG GACAGGCGCT GTGGCTCACG CCTGTAATTC CA - #GCACTTTG     1920     - GGAGGCTGAG GTAGGAGGAT TGATTGAGGC CAAGAGTTAA AGACCAGCCT GG - #TCAATATA     1980     - GCAAGACCAC GTCTCTAAAT AAAAAATAAT AGGCCGGCCA GGAAAAAAAA AA - #AAAAAAAA     2040     #           2043     - (2) INFORMATION FOR SEQ ID NO:2:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 361 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:     - Met Asp Pro Leu Gly Ala Ala Lys Pro Gln Tr - #p Pro Trp Arg Arg Cys     #                15     - Leu Ala Ala Leu Leu Phe Gln Leu Leu Val Al - #a Val Cys Phe Phe Ser     #            30     - Tyr Leu Arg Val Ser Arg Asp Asp Ala Thr Gl - #y Ser Pro Arg Ala Pro     #        45     - Ser Gly Ser Ser Arg Gln Asp Thr Thr Pro Th - #r Arg Pro Thr Leu Leu     #    60     - Ile Leu Leu Trp Thr Trp Pro Phe His Ile Pr - #o Val Ala Leu Ser Arg     #80     - Cys Ser Glu Met Val Pro Gly Thr Ala Asp Cy - #s His Ile Thr Ala Asp     #                95     - Arg Lys Val Tyr Pro Gln Ala Asp Thr Val Il - #e Val His His Trp Asp     #           110     - Ile Met Ser Asn Pro Lys Ser Arg Leu Pro Pr - #o Ser Pro Arg Pro Gln     #       125     - Gly Gln Arg Trp Ile Trp Phe Asn Leu Glu Pr - #o Pro Pro Asn Cys Gln     #   140     - His Leu Glu Ala Leu Asp Arg Tyr Phe Asn Le - #u Thr Met Ser Tyr Arg     145                 1 - #50                 1 - #55                 1 -     #60     - Ser Asp Ser Asp Ile Phe Thr Pro Tyr Gly Tr - #p Leu Glu Pro Trp Ser     #               175     - Gly Gln Pro Ala His Pro Pro Leu Asn Leu Se - #r Ala Lys Thr Glu Leu     #           190     - Val Ala Trp Ala Val Ser Asn Trp Lys Pro As - #p Ser Ala Arg Val Arg     #       205     - Tyr Tyr Gln Ser Leu Gln Ala His Leu Lys Va - #l Asp Val Tyr Gly Arg     #   220     - Ser His Lys Pro Leu Pro Lys Gly Thr Met Me - #t Glu Thr Leu Ser Arg     225                 2 - #30                 2 - #35                 2 -     #40     - Tyr Lys Phe Tyr Leu Ala Phe Glu Asn Ser Le - #u His Pro Asp Tyr Ile     #               255     - Thr Glu Lys Leu Trp Arg Asn Ala Leu Glu Al - #a Trp Ala Val Pro Val     #           270     - Val Leu Gly Pro Ser Arg Ser Asn Tyr Glu Ar - #g Phe Leu Pro Pro Asp     #       285     - Ala Phe Ile His Val Asp Asp Phe Gln Ser Pr - #o Lys Asp Leu Ala Arg     #   300     - Tyr Leu Gln Glu Leu Asp Lys Asp His Ala Ar - #g Tyr Leu Ser Tyr Phe     305                 3 - #10                 3 - #15                 3 -     #20     - Arg Trp Arg Glu Thr Leu Arg Pro Arg Ser Ph - #e Ser Trp Ala Leu Asp     #               335     - Phe Cys Lys Ala Cys Trp Lys Leu Gln Gln Gl - #u Ser Arg Tyr Gln Thr     #           350     - Val Arg Ser Ile Ala Ala Trp Phe Thr     #       360     - (2) INFORMATION FOR SEQ ID NO:3:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 1500 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: cDNA     -     (iv) ANTI-SENSE: NO     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:     - CCTTCCCTTG TAGACTCTTC TTGGAATGAG AAGTACCGAT TCTGCTGAAG AC - #CTCGCGCT       60     - CTCAGGCTCT GGGAGTTGGA ACCCTGTACC TTCCTTTCCT CTGCTGAGCC CT - #GCCTCCTT      120     - AGGCAGGCCA GAGCTCGACA GAACTCGGTT GCTTTGCTGT TTGCTTTGGA GG - #GAACACAG      180     - CTGACGATGA GGCTGACTTT GAACTCAAGA GATCTGCTTA CCCCAGTCTC CT - #GGAATTAA      240     - AGGCCTGTAC TACATTTGCC TGGACCTAAG ATTTTCATGA TCACTATGCT TC - #AAGATCTC      300     - CATGTCAACA AGATCTCCAT GTCAAGATCC AAGTCAGAAA CAAGTCTTCC AT - #CCTCAAGA      360     - TCTGGATCAC AGGAGAAAAT AATGAATGTC AAGGGAAAAG TAATCCTGTT GA - #TGCTGATT      420     - GTCTCAACCG TGGTTGTCGT GTTTTGGGAA TATGTCAACA GAATTCCAGA GG - #TTGGTGAG      480     - AACAGATGGC AGAAGGACTG GTGGTTCCCA AGCTGGTTTA AAAATGGGAC CC - #ACAGTTAT      540     - CAAGAAGACA ACGTAGAAGG ACGGAGAGAA AAGGGTAGAA ATGGAGATCG CA - #TTGAAGAG      600     - CCTCAGCTAT GGGACTGGTT CAATCCAAAG AACCGCCCGG ATGTTTTGAC AG - #TGACCCCG      660     - TGGAAGGCGC CGATTGTGTG GGAAGGCACT TATGACACAG CTCTGCTGGA AA - #AGTACTAC      720     - GCCACACAGA AACTCACTGT GGGGCTGACA GTGTTTGCTG TGGGAAAGTA CA - #TTGAGCAT      780     - TACTTAGAAG ACTTTCTGGA GTCTGCTGAC ATGTACTTCA TGGTTGGCCA TC - #GGGTCATA      840     - TTTTACGTCA TGATAGACGA CACCTCCCGG ATGCCTGTCG TGCACCTGAA CC - #CTCTACAT      900     - TCCTTACAAG TCTTTGAGAT CAGGTCTGAG AAGAGGTGGC AGGATATCAG CA - #TGATGCGC      960     - ATGAAGACCA TTGGGGAGCA CATCCTGGCC CACATCCAGC ACGAGGTCGA CT - #TCCTCTTC     1020     - TGCATGGACG TGGATCAAGT CTTTCAAGAC AACTTCGGGG TGGAAACTCT GG - #GCCAGCTG     1080     - GTAGCACAGC TCCAGGCCTG GTGGTACAAG GCCAGTCCCG AGAAGTTCAC CT - #ATGAGAGG     1140     - CGGGAACTGT CGGCCGCGTA CATTCCATTC GGAGAGGGGG ATTTTTACTA CC - #ACGCGGCC     1200     - ATTTTTGGAG GAACGCCTAC TCACATTCTC AACCTCACCA GGGAGTGCTT TA - #AGGGGATC     1260     - CTCCAGGACA AGAAACATGA CATAGAAGCC CAGTGGCATG ATGAGAGCCA CC - #TCAACAAA     1320     - TACTTCCTTT TCAACAAACC CACTAAAATC CTATCTCCAG AGTATTGCTG GG - #ACTATCAG     1380     - ATAGGCCTGC CTTCAGATAT TAAAAGTGTC AAGGTAGCTT GGCAGACAAA AG - #AGTATAAT     1440     - TTGGTTAGAA ATAATGTCTG ACTTCAAATT GTGATGGAAA CTTGACACTA TT - #TCTAACCA     1500     - (2) INFORMATION FOR SEQ ID NO:4:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 394 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:     - Met Ile Thr Met Leu Gln Asp Leu His Val As - #n Lys Ile Ser Met Ser     #                15     - Arg Ser Lys Ser Glu Thr Ser Leu Pro Ser Se - #r Arg Ser Gly Ser Gln     #            30     - Glu Lys Ile Met Asn Val Lys Gly Lys Val Il - #e Leu Leu Met Leu Ile     #        45     - Val Ser Thr Val Val Val Val Phe Trp Glu Ty - #r Val Asn Arg Ile Pro     #    60     - Glu Val Gly Glu Asn Arg Trp Gln Lys Asp Tr - #p Trp Phe Pro Ser Trp     #80     - Phe Lys Asn Gly Thr His Ser Tyr Gln Glu As - #p Asn Val Glu Gly Arg     #                95     - Arg Glu Lys Gly Arg Asn Gly Asp Arg Ile Gl - #u Glu Pro Gln Leu Trp     #           110     - Asp Trp Phe Asn Pro Lys Asn Arg Pro Asp Va - #l Leu Thr Val Thr Pro     #       125     - Trp Lys Ala Pro Ile Val Trp Glu Gly Thr Ty - #r Asp Thr Ala Leu Leu     #   140     - Glu Lys Tyr Tyr Ala Thr Gln Lys Leu Thr Va - #l Gly Leu Thr Val Phe     145                 1 - #50                 1 - #55                 1 -     #60     - Ala Val Gly Lys Tyr Ile Glu His Tyr Leu Gl - #u Asp Phe Leu Glu Ser     #               175     - Ala Asp Met Tyr Phe Met Val Gly His Arg Va - #l Ile Phe Tyr Val Met     #           190     - Ile Asp Asp Thr Ser Arg Met Pro Val Val Hi - #s Leu Asn Pro Leu His     #       205     - Ser Leu Gln Val Phe Glu Ile Arg Ser Glu Ly - #s Arg Trp Gln Asp Ile     #   220     - Ser Met Met Arg Met Lys Thr Ile Gly Glu Hi - #s Ile Leu Ala His Ile     225                 2 - #30                 2 - #35                 2 -     #40     - Gln His Glu Val Asp Phe Leu Phe Cys Met As - #p Val Asp Gln Val Phe     #               255     - Gln Asp Asn Phe Gly Val Glu Thr Leu Gly Gl - #n Leu Val Ala Gln Leu     #           270     - Gln Ala Trp Trp Tyr Lys Ala Ser Pro Glu Ly - #s Phe Thr Tyr Glu Arg     #       285     - Arg Glu Leu Ser Ala Ala Tyr Ile Pro Phe Gl - #y Glu Gly Asp Phe Tyr     #   300     - Tyr His Ala Ala Ile Phe Gly Gly Thr Pro Th - #r His Ile Leu Asn Leu     305                 3 - #10                 3 - #15                 3 -     #20     - Thr Arg Glu Cys Phe Lys Gly Ile Leu Gln As - #p Lys Lys His Asp Ile     #               335     - Glu Ala Gln Trp His Asp Glu Ser His Leu As - #n Lys Tyr Phe Leu Phe     #           350     - Asn Lys Pro Thr Lys Ile Leu Ser Pro Glu Ty - #r Cys Trp Asp Tyr Gln     #       365     - Ile Gly Leu Pro Ser Asp Ile Lys Ser Val Ly - #s Val Ala Trp Gln Thr     #   380     - Lys Glu Tyr Asn Leu Val Arg Asn Asn Val     385                 3 - #90     - (2) INFORMATION FOR SEQ ID NO:5:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 8174 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (iv) ANTI-SENSE: NO     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:     - GAATTCCATC GTGGCAAGGG CAGCCTGAAT GGATGATGTA ACCTGGGGTC CT - #TTCAATGG       60     - AGGGCCAGAC TCCTGGGTCT AGGGGATGAG GGAGGGGAGG ATCGGGTTAG CT - #GGGACCCA      120     - GGTGAAAGGG GCTGGGGGCC CACATTCCTG AGTCTCAGAG AGAAGGATCT GG - #GGTCTCAA      180     - GCACCTGAGT CGGAGGGAGG AGGGGTGCTG GGCTCCTGGA AAAACCACCT CT - #TGGACCAT      240     - CTATGCAGAT CACGCAGAAC AAGAGAAATT TCTGCGCCCC ATCTGAATTT CT - #AAGTTTGG      300     - GGGGAGGGCG TGATCTGACA CTGAGGTTCC TTGATCCTCA GCAAGGCGGC AA - #TTGCTGTA      360     - TGAAAGAAGC GACCGCATCT GAGACACAAG TATCCTGCCT TGGAAGCCTC TC - #ACCTGGCC      420     - GTGGGCCAAC CTCAACCTCA TCTGTCCCTG CTCAGATGCT CAGACCCTGG AC - #ATCCCAGC      480     - CTCCTCCTCC CTGATGCAAT CCTGGTGTTT CTTTCACCAG AGAAGCCATC CC - #AGGCCCAG      540     - GCAGGTGCTC CTGAAATAAC CTGGGGGGAG GGGTGGCTGA AAGTCCCTGA CT - #GGAGTTGG      600     - CAGCCAAGCC AGGCCCTGGA GTGGGCACCC AGAGGGAAGA CAGGTTGGCT AA - #TTTCCTGG      660     - AGCCCCTAAG GGTGCAAGGG TAGGCCTTCT GTGTCTGAGG GAGGAGGGCT GG - #GGCTCTGG      720     - ACTCCTGGGT CTGAGGGAGG AGGGGTGGGG GGCCTGGACT CCTGGGTCTG AG - #GGAGGAGG      780     - GTCTGGGCCT GTACTCCTGG ATCTGAGGGA GGAGGGGCTG GGGAACTTGG GC - #TCCTGGGT      840     - CTGAGGGAGG AGGGAGCTTT GGTCTGGACT CCTGGGTCTG AGGGAGTAGG GG - #CTAGGGAT      900     - CTGGACTCGT GGGTGTGAGG AAGGAGGGGC TGGGGTCCTG GACTCCTGGG TC - #TGAGGAAG      960     - GAGGGGCAGG GGGCTTGGAC TCCTGGGTCT GAGGAAGGAG GGGCCGGGAG CC - #TGGACTCC     1020     - TAAGTCTGAG GGAGGAGGGT CTGGGGGCCT GGACTGCTGG GTGTGAGCAG AA - #GGGTCTGG     1080     - GTGCTGGGAG TCCCGAGCCT GGGGAGATGA TGGTTAAACT TCTGGGAATC AA - #GTCAAACT     1140     - CCTGAGTCTT TGACATTGAT GTATCTTGAA TGGGAGGGTC AGTCTGTGGG GA - #AGGATTAC     1200     - CCAGGTGCCG AGGCAAGAGA CTGAAGGCAC AAACTGTTTC AGTATAATAA AG - #AAAATAGT     1260     - TAGAATAAGA ATAGTTATCA TACAAATTAG ATATAGAGAT GATCATGGAC AG - #TATCAATC     1320     - ATTAGTGTAA ACATTATTAA TCATTAGCTA TTACTTTTAT TCTTTGTTGT AT - #AACTAATA     1380     - TAACCAGGAA ACAACCGGTG GGTATAGGGT CAGGTACTGA AGGGACATTG TG - #AGAAGTGA     1440     - CCTAGAAGGC AAGAGGTGAG CCTTCTGTCA CACCGGCATA AGGGCCTCTT GA - #GGGCTCCT     1500     - TGGTCAAGCG GGAACGCCAG TGTCTGGGAA GGCACCCGTT ACTCAGCAGA CC - #ACGAAAGG     1560     - GAATCTCCTT TTCTTGGAGG AGTCAGGGAA CACTCTGCTC CACCAGCTTC TT - #GTGGGAGG     1620     - CTGGGTATTA TCTAGGCCTG CCCGCAGTCA TCCTGCTGTG CTGTGCTTCA AT - #GGTCACGC     1680     - TCCTTGTCCT CTTGCATTTT CCTCCCGTAC TCCTGGTTCC TCTTTGAAGT TC - #GTAGTAGA     1740     - TAGCGGTAGA AGAAATAGTG AAAGCCTTTT TTTTTTTTTT TTTGAGGCGG AG - #TCTCGCTC     1800     - TGTCCCCCAG GCTGGAGTGC AGTGGCGTGA TCTCGGCTCA CTGCAATCTC CG - #CCTCCTGG     1860     - GTTCACACCA TTCTCCTGCC TCACCCTCCC AAATAGCTAG GACTACAGGC GC - #CCTCCACC     1920     - ACGCGCCCGG ATAATTTTTT GTATTTTTAG TAGAGACAGG GTTTCACCGT GT - #TAGCCAGG     1980     - ATGGCCTCCA CCTCCTGACC TTGTGATCCG CCCGCCTCAG CCTCCCAAAG TG - #CTGGGATT     2040     - ACAGGCGTGA GCCACCGCGC CCGCCCGAAA TAGTGAAAGT CTTAAAGTCT TT - #GATCTTTC     2100     - TTATAAGTGC AGAGAAGAAA ACGCTGACAT ATGCTGCCTT CTCTTTCTGC TT - #CGGCTGCC     2160     - TAAAAGGGAA GGGCCCCCTG TCCCATGATC ACGTGACTTG CTTGACCTTA TC - #AGTCATTT     2220     - GGACGACTCA CCCTCCTTAT CCTGCCCCCC CTTGTCTTGT ATACAATAAA TA - #TCAGCGCG     2280     - CCCAGCCATT CGGGGCCACT ACCGGTCTCT GCGTCTTGAT GGTAGTGGTC CC - #CCGGGCCC     2340     - AGCTGTTTTC TCTTTATCTC TTTGTCTTGT GTCTTTATTT CTTACAATCT CT - #CCTCTCCT     2400     - CACAGGGGAA GAACACCCAC CCGCAAAGCC CCGTAGGGCT GGACCCTACG TT - #AGCCTGCC     2460     - CTGCTCGGGG TTGGCGATGC TGGAGGTGGG CCTTGGACCA GAGAAAATGC TT - #TAATTAGG     2520     - TGACAAGCGG GCAGAGGCCT TTGTCTCTGG CGCCGGCAGC CACGGCCCCC GC - #TGACGGCG     2580     - TGGGAAACAG ACCCTGTTCC ACTCCGGTCT CCAGCCTTGG AATGGTTGCC TT - #CGTGCAGT     2640     - GCAGGTCTGG AAAGTAGCAG TTTGGCACGG GACCCTAGAA TTCCCCAAAA GG - #AGTGACTA     2700     - GGGGCTGGGA TTCTGGAATT TGAGTGTGGA CGGTGAGGCG GGGGGTGTGG GA - #GATCGGAG     2760     - ACCCTGGTGG GCGCGGGAGC ACCTGCAGGC TGGAGGCCCT CGCGCGCTCC GG - #CGGCAGCC     2820     - TGGCAAACAG GTTCTCCATC CCCCAGGAGG ACGCGGCAGA GGGCGGACGA TC - #GCTCCACT     2880     - CGCCGGGACC AGGTGCGGGG GCCCTGCCCA GCCGCTGGGG CGTGGCCAGG CT - #CGAAGCAC     2940     - CCAGGTGTCG GGGGCCGACT CTAAGCCCTG GCACCGGAAG AGAGAGGGCG GC - #GGATTGGA     3000     - CCTCCCGGCT CCAGCATTGC AACTGGGCGC TCCGTCTCCT GGTCCACGCA AT - #GATGCTGC     3060     - GGCTGCTCAG AAGCCAGGTA GCCTGCCCTG GGTGAAGCCT TCGCGCAGGT CA - #ATGACGGG     3120     - GCGGAGGGGC AGGGCGCGGT CCCCTGCATC CCCGATCTGG GGAGCGGTGG GC - #CCAGGGGC     3180     - CATCGCCTTA GCCCCTGGCG CTGGGGCTCG GCGCCAAGTG ACGGGCGGGG CT - #CCACCTTC     3240     - CAGCCATCCG CCCGGCCCGG GAGGGCGGAC GCTGCGAGAC TCCCGGCCGC GC - #CCTCTCCT     3300     - TCCTCTCCTC CCCAAGCCCT CGCTGCCAGT CCGGACAGGC TGCGCGGAGG GG - #AGGGCTGC     3360     - CGGGCCGGAT AGCCGGACGC CTGGCGTTCC AGGGGCGGCC GGATGTGGCC TG - #CCTTTGCG     3420     - GAGGGTGCGC TCCGGCCACG AAAAGCGGAC TGTGGATCTG CCACCTGCAA GC - #AGCTCGGG     3480     - TAAGTGGGGA CTGCCCCACT CAGTTGTTCC TGGGACCCAG GAACAACTCC TT - #CAGAACCA     3540     - GGAGGTGCAC CCCCAACCTC TTCTCCAGGT CTTCCTAAGG CCCTAGGAAT CT - #CCGCCACC     3600     - TCCCCAGCCA TTACTCCTCC AGGAACCAAG ATGCTCCTTC CGCTCCTGAC CC - #TCCAGCCT     3660     - CTCTTGTTTT ACTTGAACTA TCGTTTCCCA TCACCACCTC TGTGGTGGAT TT - #TGCGCCTC     3720     - ACAGACAGGT ACTCCTGAGA AACAGGCTGG TGGAAGAGTC CAGTATCAGC GG - #AACTTACA     3780     - GGAGGGGAGA CTCGAGATTC CTTCAGGAAA GGTGTAGGAA CCTGGACCAC TT - #TCTTTTTT     3840     - TTTTTTTTTT TTTTTTTAAG ACAGGGTCCC TCTCTGTCGC GCAAGCTGGA GT - #GCAGTCAG     3900     - CGGTGCTATC GCGGCTCATT GTGAGCTCCG GGGATCCTCC CGCCTTAGCA TC - #CGGTGTAG     3960     - CTGAGACCAC AGACATGTGC CACCATGCCA AGCTAATTTT ATTTATTTTT TT - #TTGGAGAC     4020     - GGAGTTTCAC TCTTGTTGCC CAGGCTGGAG TGTAATGGCA TGATCTCAGC TC - #ACCGCAAC     4080     - TCCCGCCCCC CGGGTTCAGG CGATTCTCCT GCCTCAGCCT CCCGAGTGGC TG - #GGATTACA     4140     - GGCATGCGCC ACCATGCCCG GCTAATTTTG TATTTTAAGT AGAGACAGGG TT - #TCTCCACG     4200     - TTGGTCAGGC TGGTCTCGAA CTCCCAACCT CAGGTGATCC ACCCACCTTG GC - #CTCCCAAA     4260     - GTGCTGGGAT TACAGGTGTG AGCCACCGCG CCTGGCCCAT GCCAAGCTAA TT - #TTAAAATT     4320     - TTTTTGTAAG AGTGCTCTGT TGCCCAGGCT GATCTTGAAC TCCTGGGCTC AA - #GGGATCCT     4380     - CCCATCTCAG CCTCCCAATA TGCTGGGATT ACAGGTGTGA GCCACAGTGC CC - #AGCCAAAC     4440     - CATGGCTATC TTGAAAACCA CTTGTCTTCC AGTCCCCATG CCCCGAAATT CC - #AAGGCTCT     4500     - CATCCCTGAA ACCTAGGACT CAGGCTCTCC CTACCTCAGC CCCAGGAGTC TA - #AACCTTTA     4560     - ACTTCCTCTT TCCCTGGGAC TAAGGAGTGC TGCACCCCAG GCGCCTCCCT TA - #CCCCACAT     4620     - CCCTCCTCAG CCTCCCCTCC TCAGCCTCAG TGCATTTGCT AATTCGCCTT TC - #CTCCCCTG     4680     - CAGCCATGTG GCTCCGGAGC CATCGTCAGC TCTGCCTGGC CTTCCTGCTA GT - #CTGTGTCC     4740     - TCTCTGTAAT CTTCTTCCTC CATATCCATC AAGACAGCTT TCCACATGGC CT - #AGGCCTGT     4800     - CGATCCTGTG TCCAGACCGC CGCCTGGTGA CACCCCCAGT GGCCATCTTC TG - #CCTGCCGG     4860     - GTACTGCGAT GGGCCCCAAC GCCTCCTCTT CCTGTCCCCA GCACCCTGCT TC - #CCTCTCCG     4920     - GCACCTGGAC TGTCTACCCC AATGGCCGGT TTGGTAATCA GATGGGACAG TA - #TGCCACGC     4980     - TGCTGGCTCT GGCCCAGCTC AACGGCCGCC GGGCCTTTAT CCTGCCTGCC AT - #GCATGCCG     5040     - CCCTGGCCCC GGTATTCCGC ATCACCCTGC CCGTGCTGGC CCCAGAAGTG GA - #CAGCCGCA     5100     - CGCCGTGGCG GGAGCTGCAG CTTCACGACT GGATGTCGGA GGAGTACGCG GA - #CTTGAGAG     5160     - ATCCTTTCCT GAAGCTCTCT GGCTTCCCCT GCTCTTGGAC TTTCTTCCAC CA - #TCTCCGGG     5220     - AACAGATCCG CAGAGAGTTC ACCCTGCACG ACCACCTTCG GGAAGAGGCG CA - #GAGTGTGC     5280     - TGGGTCAGCT CCGCCTGGGC CGCACAGGGG ACCGCCCGCG CACCTTTGTC GG - #CGTCCACG     5340     - TGCGCCGTGG GGACTATCTG CAGGTTATGC CTCAGCGCTG GAAGGGTGTG GT - #GGGCGACA     5400     - GCGCCTACCT CCGGCAGGCC ATGGACTGGT TCCGGGCACG GCACGAAGCC CC - #CGTTTTCG     5460     - TGGTCACCAG CAACGGCATG GAGTGGTGTA AAGAAAACAT CGACACCTCC CA - #GGGCGATG     5520     - TGACGTTTGC TGGCGATGGA CAGGAGGCTA CACCGTGGAA AGACTTTGCC CT - #GCTCACAC     5580     - AGTGCAACCA CACCATTATG ACCATTGGCA CCTTCGGCTT CTGGGCTGCC TA - #CCTGGCTG     5640     - GCGGAGACAC TGTCTACCTG GCCAACTTCA CCCTGCCAGA CTCTGAGTTC CT - #GAAGATCT     5700     - TTAAGCCGGA GGCGGCCTTC CTGCCCGAGT GGGTGGGCAT TAATGCAGAC TT - #GTCTCCAC     5760     - TCTGGACATT GGCTAAGCCT TGAGAGCCAG GGAGACTTTC TGAAGTAGCC TG - #ATCTTTCT     5820     - AGAGCCAGCA GTACGTGGCT TCAGAGGCCT GGCATCTTCT GGAGAAGCTT GT - #GGTGTTCC     5880     - TGAAGCAAAT GGGTGCCCGT ATCCAGAGTG ATTCTAGTTG GGAGAGTTGG AG - #AGAAGGGG     5940     - GACGTTTCTG GAACTGTCTG AATATTCTAG AACTAGCAAA ACATCTTTTC CT - #GATGGCTG     6000     - GCAGGCAGTT CTAGAAGCCA CAGTGCCCAC CTGCTCTTCC CAGCCCATAT CT - #ACAGTACT     6060     - TCCAGATGGC TGCCCCCAGG AATGGGGAAC TCTCCCTCTG GTCTACTCTA GA - #AGAGGGGT     6120     - TACTTCTCCC CTGGGTCCTC CAAAGACTGA AGGAGCATAT GATTGCTCCA GA - #GCAAGCAT     6180     - TCACCAAGTC CCCTTCTGTG TTTCTGGAGT GATTCTAGAG GGAGACTTGT TC - #TAGAGAGG     6240     - ACCAGGTTTG ATGCCTGTGA AGAACCCTGC AGGGCCCTTA TGGACAGGAT GG - #GGTTCTGG     6300     - AAATCCAGAT AACTAAGGTG AAGAATCTTT TTAGTTTTTT TTTTTTTTTT TT - #GGAGACAG     6360     - GGTCTCGCTC TGTTGCCCAG GCTGGAGTGC AGTGGCGTGA TCTTGGCTCA CT - #GCAACTTC     6420     - CGCCTCCTGT GTTCAAGCGA TTCTCCTGTC TCAGCCTCCT GAGTAGATGG GA - #CTACAGGC     6480     - ACAGGCCATT ATGCCTGGCT AATTTTTGTA TTTTTAGTAG AGACAGGGTT TC - #ACCATGTT     6540     - GGCCGGGATG GTCTCGATCT CCTGACCTTG TCATCCACCT GTCTTGGCCT CC - #CAAAGTGC     6600     - TGGGATTACT GGCATGAGCC ACTGTGCCCA GCCCGGATAT TTTTTTTTAA TT - #ATTTATTT     6660     - ATTTATTTAT TTATTGAGAC GGAGTCTTGC TCTGTAGCCC AGGCCAGAGT GC - #AGTGGCGC     6720     - GATCTCAGCT CACTGCAAGC TCTGCCTCCC GGGTTCATGC CATTCTGCCT CA - #GCCTCCTG     6780     - AGTAGCTGGG ACTACAGGCG CCCGCCACCA CGCCCGGCTA ATTTTTTTTG TA - #TTTTTAGT     6840     - AGAGACGGGG TTTCATCGTG TTAACCAGGA TGGTCTCGAT CTCCTGACCT CG - #TGATCTGC     6900     - CCACCTCGGC CTCCCACAGT GCTGGGATTA CCGGCGTGAG CCACCATGCC TG - #GCCCGGAT     6960     - AATTTTTTTT AATTTTTGTA GAGACGAGGT CTTGTGATAT TGCCCAGGCT GT - #TCTTCAAC     7020     - TCCTGGGCTC AAGCAGTCCT CCCACCTTGG CCTCCCAGAA TGCTGGGTTT AT - #AGATGTGA     7080     - GCCAGCACAC CGGGCCAAGT GAAGAATCTA ATGAATGTGC AACCTAATTG TA - #GCATCTAA     7140     - TGAATGTTCC ACCATTGCTG GAAAAATTGA GATGGAAAAC AAACCATCTC TA - #GTTGGCCA     7200     - GCGTCTTGCT CTGTTCACAG TCTCTGGAAA AGCTGGGGTA GTTGGTGAGC AG - #AGCGGGAC     7260     - TCTGTCCAAC AAGCCCCACA GCCCCTCAAA GACTTTTTTT TGTTTGTTTT GA - #GCAGACAG     7320     - GCTAAAATGT GAACGTGGGG TGAGGGATCA CTGCCAAAAT GGTACAGCTT CT - #GGAGCAGA     7380     - ACTTTCCAGG GATCCAGGGA CACTTTTTTT TAAAGCTCAT AAACTGCCAA GA - #GCTCCATA     7440     - TATTGGGTGT GAGTTCAGGT TGCCTCTCAC AATGAAGGAA GTTGGTCTTT GT - #CTGCAGGT     7500     - GGGCTGCTGA GGGTCTGGGA TCTGTTTTCT GGAAGTGTGC AGGTATAAAC AC - #ACCCTCTG     7560     - TGCTTGTGAC AAACTGGCAG GTACCGTGCT CATTGCTAAC CACTGTCTGT CC - #CTGAACTC     7620     - CCAGAACCAC TACATCTGGC TTTGGGCAGG TCTGAGATAA AACGATCTAA AG - #GTAGGCAG     7680     - ACCCTGGACC CAGCCTCAGA TCCAGGCAGG AGCACGAGGT CTGGCCAAGG TG - #GACGGGGT     7740     - TGTCGAGATC TCAGGAGCCC CTTGCTGTTT TTTGGAGGGT GAAAGAAGAA AC - #CTTAAACA     7800     - TAGTCAGCTC TGATCACATC CCCTGTCTAC TCATCCAGAC CCCATGCCTG TA - #GGCTTATC     7860     - AGGGAGTTAC AGTTACAATT GTTACAGTAC TGTTCCCAAC TCAGCTGCCA CG - #GGTGAGAG     7920     - AGCAGGAGGT ATGAATTAAA AGTCTACAGC ACTAACCCGT GTCTCTGTAG CT - #TTTTTGGA     7980     - GCCAGAGCCA CTGTGTATGT GTGTGTGGGT TTGTGTGTGT GTGTGTGTGT GT - #GTGTGTGT     8040     - AAGAGAGTGG AGGAAAAGGT GGGGTACTTC TGAAGACTTT TATTTTTTTT TA - #ATTAATTT     8100     - ATTTTTTTTC AGAGATCGAG TCTTGCTCTG TGGCCCAGGC TGGAGTGCAG TA - #GTGTGATC     8160     #   8174     - (2) INFORMATION FOR SEQ ID NO:6:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 365 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:     - Met Trp Leu Arg Ser His Arg Gln Leu Cys Le - #u Ala Phe Leu Leu Val     #                15     - Cys Val Leu Ser Val Ile Phe Phe Leu His Il - #e His Gln Asp Ser Phe     #            30     - Pro His Gly Leu Gly Leu Ser Ile Leu Cys Pr - #o Asp Arg Arg Leu Val     #        45     - Thr Pro Pro Val Ala Ile Phe Cys Leu Pro Gl - #y Thr Ala Met Gly Pro     #    60     - Asn Ala Ser Ser Ser Cys Pro Gln His Pro Al - #a Ser Leu Ser Gly Thr     #80     - Trp Thr Val Tyr Pro Asn Gly Arg Phe Gly As - #n Gln Met Gly Gln Tyr     #                95     - Ala Thr Leu Leu Ala Leu Ala Gln Leu Asn Gl - #y Arg Arg Ala Phe Ile     #           110     - Leu Pro Ala Met His Ala Ala Leu Ala Pro Va - #l Phe Arg Ile Thr Leu     #       125     - Pro Val Leu Ala Pro Glu Val Asp Ser Arg Th - #r Pro Trp Arg Glu Leu     #   140     - Gln Leu His Asp Trp Met Ser Glu Glu Tyr Al - #a Asp Leu Arg Asp Pro     145                 1 - #50                 1 - #55                 1 -     #60     - Phe Leu Lys Leu Ser Gly Phe Pro Cys Ser Tr - #p Thr Phe Phe His His     #               175     - Leu Arg Glu Gln Ile Arg Arg Glu Phe Thr Le - #u His Asp His Leu Arg     #           190     - Glu Glu Ala Gln Ser Val Leu Gly Gln Leu Ar - #g Leu Gly Arg Thr Gly     #       205     - Asp Arg Pro Arg Thr Phe Val Gly Val His Va - #l Arg Arg Gly Asp Tyr     #   220     - Leu Gln Val Met Pro Gln Arg Trp Lys Gly Va - #l Val Gly Asp Ser Ala     225                 2 - #30                 2 - #35                 2 -     #40     - Tyr Leu Arg Gln Ala Met Asp Trp Phe Arg Al - #a Arg His Glu Ala Pro     #               255     - Val Phe Val Val Thr Ser Asn Gly Met Glu Tr - #p Cys Lys Glu Asn Ile     #           270     - Asp Thr Ser Gln Gly Asp Val Thr Phe Ala Gl - #y Asp Gly Gln Glu Ala     #       285     - Thr Pro Trp Lys Asp Phe Ala Leu Leu Thr Gl - #n Cys Asn His Thr Ile     #   300     - Met Thr Ile Gly Thr Phe Gly Phe Trp Ala Al - #a Tyr Leu Ala Gly Gly     305                 3 - #10                 3 - #15                 3 -     #20     - Asp Thr Val Tyr Leu Ala Asn Phe Thr Leu Pr - #o Asp Ser Glu Phe Leu     #               335     - Lys Ile Phe Lys Pro Glu Ala Ala Phe Leu Pr - #o Glu Trp Val Gly Ile     #           350     - Asn Ala Asp Leu Ser Pro Leu Trp Thr Leu Al - #a Lys Pro     #       365     - (2) INFORMATION FOR SEQ ID NO:7:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 3647 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (iv) ANTI-SENSE: NO     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:     - CTGCAGAGAG CGCCACCCGG AAGCCACTTT TATAGAAGCT TTTACACACA AT - #GCTTGATT       60     - TTTTTTTTTT TTTTCCGAGA CGGAGTCTCG CTTTGTCGCC CAGGCTGGAG TG - #CAGTGGCG      120     - CGATCTGGGC TCACTGCAAG CTCCGCCTCC TGGGTTGACG CCATTCTCCT GC - #CTCAGCTT      180     - CCCGAGTAGC TGGGACTACA GGCGCCCGCC ACCAAGCCTG GCTAATTTTT TT - #TTATTTTT      240     - AGTGGAGACA GAGTTTCACC GTGTTAGCCA GGATGGTCTC GATCTCCTGA CC - #TCGGGATC      300     - CGCCCGCCTC GGCCTCCCAA AGTGCTGGGA GTATAGGCGT GAGCCACCGC GC - #CTGGCCTA      360     - TACTTGATTT TTAATGAAAA CATTCTTAAA TTCATATGGC TAACGCAAAT TT - #ATTTTCTG      420     - TAGGCATAAC ATCAAAAACA CCTGGCAGGA CTGCCCCATT CCCAGCACTG TC - #TAGTTCTC      480     - CCCTAGTATC AGTGGGACTC CACTGATGCA CAGCTGTGAT CTACTAAAAC TT - #CTCTCAAA      540     - ACTTTCTCCT CTCCTTAGGT CAGCAGCCCC GCCCCTGATC TATTTGGAAA TC - #CCCTGAAT      600     - AAAAGTTGAA TATCATAAAC CAAAGCGAAC ACCCAGAAAT TCAAATTCAA CC - #CGTAGGTA      660     - AAAAATTTCT CAAGTGACTG TAGACGTAGA TGTCTCCAGT GTCGCCTAAT AA - #GGTAGAAG      720     - AGGCCAGTGC GATACTGTCT TTACACCCTT AACTTGGGTG CTAGAATATT TA - #TCTTCGTC      780     - ATCATTTTAT CATCCAAACT ATTTTGCATA ACTTTCATGG GTGCAGAAAA TG - #TTTTTTAA      840     - GTGCTTGGTA AAATTAATAG TGATATTCAT TCATTCATCT CACTGAACAG GC - #AATAAATT      900     - CCTTGACGAC AAGGGCCTTG GGGGGGGCCA CATCTTCATC TTTGGTTTAT GA - #GTCCTGTG      960     - CGTCTTGGTA CAAGCAATAC TACTATGAGC CGGCAAGTCA GACTTATTTG GT - #AGGGGACC     1020     - AAAGGAAAGA ACATGTTTTG ATTGCTAAGA AAACATTTTG TTCTCTATCC TT - #TACTGGGC     1080     - TGGCAGGCAA AGGAAATGTT CTTATGAGCA CTCACATTGA AAACTTAAGT TC - #TTCACCAA     1140     - ATGCAGAGAC TCTGAAGGCC ACGCCGCTGC GGGCTGCCTC CACAATTCGA CC - #GTCTCGGC     1200     - GGGCCACGAG ATCCTGGCCA CGGATGCGGT GGCCGCGCCT CTGCTCGCAC GT - #TCCCCCGG     1260     - CCTCTGGACT CCCTCCCTCC CTCAATCCCT CCCTCCGGCG GGCGTCGCTG GC - #GGGTGGCT     1320     - AGGCCCAACG GCAGGAAGCC GACGCTATCC TCCGTTCCGC GGCGCCGGGT CC - #GCCTTCCG     1380     - TCTGTTCTAG GGCCTGCTCC TGCGCGGCAG CTGCTTTAGA AGGTCTCGAG CC - #TCCTGTAC     1440     - CTTCCCAGGG ATGAACCGGG CCTTCCCTCT GGAAGGCGAG GGTTCGGGCC AC - #AGTGAGCG     1500     - AGGGCCAGGG CGGTGGGCGC GCGCAGAGGG AAACCGGATC AGTTGAGAGA GA - #ATCAAGAG     1560     - TAGCGGATGA GGCGCTTGTG GGGCGCGGCC CGGAAGCCCT CGGGCGCGGG CT - #GGGAGAAG     1620     - GAGTGGGCGG AGGCGCCGCA GGAGGCTCCC GGGGCCTGGT CGGGCCGGCT GG - #GCCCCGGG     1680     - CGCAGTGGAA GAAAGGGACG GGCGGTGCCC GGTTGGGCGT CCTGGCCAGC TC - #ACCTTGCC     1740     - CTGGCGGCTC GCCCCGCCCG GCACTTGGGA GGAGCAGGGC AGGGCCCGCG GC - #CTTTGCAT     1800     - TCTGGGACCG CCCCCTTCCA TTCCCGGGCC AGCGGCGAGC GGCAGCGACG GC - #TGGAGCCG     1860     - CAGCTACAGC ATGAGAGCCG GTGCCGCTCC TCCACGCCTG CGGACGCGTG GC - #GAGCGGAG     1920     - GCAGCGCTGC CTGTTCGCGC CATGGGGGCA CCGTGGGGCT CGCCGACGGC GG - #CGGCGGGC     1980     - GGGCGGCGCG GGTGGCGCCG AGGCCGGGGG CTGCCATGGA CCGTCTGTGT GC - #TGGCGGCC     2040     - GCCGGCTTGA CGTGTACGGC GCTGATCACC TACGCTTGCT GGGGGCAGCT GC - #CGCCGCTG     2100     - CCCTGGGCGT CGCCAACCCC GTCGCGACCG GTGGGCGTGC TGCTGTGGTG GG - #AGCCCTTC     2160     - GGGGGGCGCG ATAGCGCCCC GAGGCCGCCC CCTGACTGCC CGCTGCGCTT CA - #ACATCAGC     2220     - GGCTGCCGCC TGCTCACCGA CCGCGCGTCC TACGGAGAGG CTCAGGCCGT GC - #TTTTCCAC     2280     - CACCGCGACC TCGTGAAGGG GCCCCCCGAC TGGCCCCCGC CCTGGGGCAT CC - #AGGCGCAC     2340     - ACTGCCGAGG AGGTGGATCT GCGCGTGTTG GACTACGAGG AGGCAGCGGC GG - #CGGCAGAA     2400     - GCCCTGGCGA CCTCCAGCCC CAGGCCCCCG GGCCAGCGCT GGGTTTGGAT GA - #ACTTCGAG     2460     - TCGCCCTCGC ACTCCCCGGG GCTGCGAAGC CTGGCAAGTA ACCTCTTCAA CT - #GGACGCTC     2520     - TCCTACCGGG CGGACTCGGA CGTCTTTGTG CCTTATGGCT ACCTCTACCC CA - #GAAGCCAC     2580     - CCCGGCGACC CGCCCTCAGG CCTGGCCCCG CCACTGTCCA GGAAACAGGG GC - #TGGTGGCA     2640     - TGGGTGGTGA GCCACTGGGA CGACCGCCAG GCCCGGGTCC GCTACTACCA CC - #AACTGAGC     2700     - CAACATGTGA CCGTGGACGT GTTCGGCCGG GGCGGGCCGG GGCAGCCGGT GC - #CCGAAATT     2760     - GGGCTCCTGC ACACAGTGGC CCGCTACAAG TTCTACCTGG CTTTCGAGAA CT - #CGCAGCAC     2820     - CTGGATTATA TCACCGAGAA GCTCTGGCGC AACGCGTTGC TCGCTGGGGC GG - #TGCCGGTG     2880     - GTGCTGGGCC CAGACCGTGC CAACTACGAG GCGTTTGTGC CCCGCGGCGC CT - #TCATCCAC     2940     - GTGGACGACT TCCCAAGTGC CTCCTCCCTG GCCTCGTACC TGCTTTTCCT CG - #ACCGCAAC     3000     - CCCGCGGTCT ATCGCCGCTA CTTCCACTGG CGCCGGAGCT ACGCTGTCCA CA - #TCACCTCC     3060     - TTCTGGGACG AGCCTTGGTG CCGGGTGTGC CAGGCTGTAC AGAGGGCTGG GG - #ACCGGCCC     3120     - AAGAGCATAC GGAACTTGGC CAGCTGGTTC GAGCGGTGAA GCCGCGCTCC CC - #TGGAAGCG     3180     - ACCCAGGGGA GCCCAAGTTG TCAGCTTTTT GATCCTCTAC TGTGCATCTC CT - #TGACTGCC     3240     - GCATCATGGG AGTAAGTTCT TCAAACACCC ATTTTTGCTC TATGGGAAAA AA - #ACGATTTA     3300     - CCAATTAATA TTACTCAGCA CAGAGATGGG GGCCCGGTTT CCATATTTTT TG - #CACAGCTA     3360     - GCAATTGGGC TCCCTTTGCT GCTGATGGGC ATCATTGTTT AGGGGTGAAG GA - #GGGGGTTC     3420     - TTCCTCACCT TGTAACCAGT GCAGAAATGA AATAGCTTAG CGGCAAGAAG CC - #GTTGAGGC     3480     - GGTTTCCTGA ATTTCCCCAT CTGCCACAGG CCATATTTGT GGCCCGTGCA GC - #TTCCAAAT     3540     - CTCATACACA ACTGTTCCCG ATTCACGTTT TTCTGGACCA AGGTGAAGCA AA - #TTTGTGGT     3600     #              3647TTGG TGGAGAGTGG AAGGACTGTG GCTGCAG     - (2) INFORMATION FOR SEQ ID NO:8:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 405 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:     - Met Gly Ala Pro Trp Gly Ser Pro Thr Ala Al - #a Ala Gly Gly Arg Arg     #                15     - Gly Trp Arg Arg Gly Arg Gly Leu Pro Trp Th - #r Val Cys Val Leu Ala     #            30     - Ala Ala Gly Leu Thr Cys Thr Ala Leu Ile Th - #r Tyr Ala Cys Trp Gly     #        45     - Gln Leu Pro Pro Leu Pro Trp Ala Ser Pro Th - #r Pro Ser Arg Pro Val     #    60     - Gly Val Leu Leu Trp Trp Glu Pro Phe Gly Gl - #y Arg Asp Ser Ala Pro     #80     - Arg Pro Pro Pro Asp Cys Pro Leu Arg Phe As - #n Ile Ser Gly Cys Arg     #                95     - Leu Leu Thr Asp Arg Ala Ser Tyr Gly Glu Al - #a Gln Ala Val Leu Phe     #           110     - His His Arg Asp Leu Val Lys Gly Pro Pro As - #p Trp Pro Pro Pro Trp     #       125     - Gly Ile Gln Ala His Thr Ala Glu Glu Val As - #p Leu Arg Val Leu Asp     #   140     - Tyr Glu Glu Ala Ala Ala Ala Ala Glu Ala Le - #u Ala Thr Ser Ser Pro     145                 1 - #50                 1 - #55                 1 -     #60     - Arg Pro Pro Gly Gln Arg Trp Val Trp Met As - #n Phe Glu Ser Pro Ser     #               175     - His Ser Pro Gly Leu Arg Ser Leu Ala Ser As - #n Leu Phe Asn Trp Thr     #           190     - Leu Ser Tyr Arg Ala Asp Ser Asp Val Phe Va - #l Pro Tyr Gly Tyr Leu     #       205     - Tyr Pro Arg Ser His Pro Gly Asp Pro Pro Se - #r Gly Leu Ala Pro Pro     #   220     - Leu Ser Arg Lys Gln Gly Leu Val Ala Trp Va - #l Val Ser His Trp Asp     225                 2 - #30                 2 - #35                 2 -     #40     - Glu Arg Gln Ala Arg Val Arg Tyr Tyr His Gl - #n Leu Ser Gln His Val     #               255     - Thr Val Asp Val Phe Gly Arg Gly Gly Pro Gl - #y Gln Pro Val Pro Glu     #           270     - Ile Gly Leu Leu His Thr Val Ala Arg Tyr Ly - #s Phe Tyr Leu Ala Phe     #       285     - Glu Asn Ser Gln His Leu Asp Tyr Ile Thr Gl - #u Lys Leu Trp Arg Asn     #   300     - Ala Leu Leu Ala Gly Ala Val Pro Val Val Le - #u Gly Pro Asp Arg Ala     305                 3 - #10                 3 - #15                 3 -     #20     - Asn Tyr Glu Arg Phe Val Pro Arg Gly Ala Ph - #e Ile His Val Asp Asp     #               335     - Phe Pro Ser Ala Ser Ser Leu Ala Ser Tyr Le - #u Leu Phe Leu Asp Arg     #           350     - Asn Pro Ala Val Tyr Arg Arg Tyr Phe His Tr - #p Arg Arg Ser Tyr Ala     #       365     - Val His Ile Thr Ser Phe Trp Asp Glu Pro Tr - #p Cys Arg Val Cys Gln     #   380     - Ala Val Gln Arg Ala Gly Asp Arg Pro Lys Se - #r Ile Arg Asn Leu Ala     385                 3 - #90                 3 - #95                 4 -     #00     - Ser Trp Phe Glu Arg                     405     - (2) INFORMATION FOR SEQ ID NO:9:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 1488 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:     - ATGGGGGCAC CGTGGGGCTC GCCGACGGCG GCGGCGGGCG GGCGGCGCGG GT - #GGCGCCGA       60     - GGCCCGGGGC TGCCATGGAC CGTCTGTGTG CTGGCGGCCG CCGGCTTGAC GT - #GTACGGCG      120     - CTGATCACCT ACGCTTGCTG GGGGCAGCTG CCGCCGCTGC CCTGGGCGTC GC - #CAACCCCG      180     - TCGCGACCGG TGGGCGTGCT GCTGTGGTGG GAGCCCTTCG GGGGGCGCGA TA - #GCGCCCCG      240     - AGGCCGCCCC CTGACTGCTG CTGGGGGCAG CTGCCGCCGC TGCCCTGGGC GT - #CGCCAACC      300     - CCGTCGCGAC CGGTGGGCGT GCTGCTGTGG TGGGAGCCCT TCGGGGGGCG CG - #ATAGCGCC      360     - CCGAGGCCGC CCCCTGACTG CCCGCTGCGC TTCAACATCA GCGGCTGCCG CC - #TGCTCACC      420     - GACCGCGCGT CCTACGGAGA GGCTCAGGCC GTGCTTTTCC ACCACCGCGA CC - #TCGTGAAG      480     - GGGCCCCCCG ACTGGCCCCC GCCCTGGGGC ATCCAGGCGC ACACTGCCGA GC - #CGCTGCGC      540     - TTCAACATCA GCGGCTGCCG CCTGCTCACC GACCGCGCGT CCTACGGAGA GG - #CTCAGGCC      600     - GTGCTTTTCC ACCACCGCGA CCTCGTGAAG GGGCCCCCCG ACTGGCCCCC GC - #CCTGGGGC      660     - ATCCAGGCGC ACACTGCCGA GGAGGTGGAT CTGCGCGTGT TGGACTACGA GG - #AGGCAGCG      720     - GCGGCGGCAG AAGCCCTGGC GACCTCCAGC CCCAGGCCCC CGGGCCAGCG CT - #GGGTTTGG      780     - ATGAACTTCG AGTCGCCCTC GCACTCCCCG GGGCTGCGAA GCCTGGCAAG TA - #ACCTCTTC      840     - AACTGGACGC TCTCCTACCG GGCGGACTCG GACGTCTTTG TGCCTTATGG CT - #ACCTCTAC      900     - CCCAGAAGCC ACCCCGGCGA CCCGCCCTCA GGCCTGGCCC CGCCACTGTC CA - #GGAAACAG      960     - GGGCTGGTGG CATGGGTGGT GAGCCACTGG GACGAGCGCC AGGCCCGGGT CC - #GCTACTAC     1020     - CACCAACTGA GCCAACATGT GACCGTGGAC GTGTTCGGCC GGGGCGGGCC GG - #GGCAGCCG     1080     - GTGCCCGAAA TTGGGCTCCT GCACACAGTG GCCCGCTACA AGTTCTACCT GG - #CTTTCGAG     1140     - AACTCGCAGC ACCTGGATTA TATCACCGAG AAGCTCTGGC GCAACGCGTT GC - #TCGCTGGG     1200     - GCGGTGCCGG TGGTGCTGGG CCCAGACCGT GCCAACTACG AGCGCTTTGT GC - #CCCGCGGC     1260     - GCCTTCATCC ACGTGGACGA CTTCCCAAGT GCCTCCTCCC TGGCCTCGTA CC - #TGCTTTTC     1320     - CTCGACCGCA ACCCCGCGGT CTATCGCCGC TACTTCCACT GGCGCCGGAG CT - #ACGCTGTC     1380     - CACATCACCT CCTTCTGGGA CGAGCCTTGG TGCCGGGTGT GCCAGGCTGT AC - #AGAGGGCT     1440     #              1488GCAT ACGGAACTTG GCCAGCTGGT TCGAGCGG     - (2) INFORMATION FOR SEQ ID NO:10:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 1316 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:     - TTTATGACAA GCTGTGTCAT AAATTATAAC AGCTTCTCTC AGGACACTGT GG - #CCAGGAAG       60     - TGGGTGATCT TCCTTAATGA CCCTCACTCC TCTCTCCTCT CTTCCCAGCT AC - #TCTGACCC      120     - ATGGATCCCC TGGGCCCAGC CAAGCCACAG TGGCTGTGGC GCCGCTGTCT GG - #CCGGGCTG      180     - CTGTTTCAGC TGCTGGTGGC TGTGTGTTTC TTCTCCTACC TGCGTGTGTC CC - #GAGACGAT      240     - GCCACTGGAT CCCCTAGGCC AGGGCTTATG GCAGTGGAAC CTGTCACCGG GG - #CTCCCAAT      300     - GGGTCCCGCT GCCAGGACAG CATGGCGACC CCTGCCCACC CCACCCTACT GA - #TCCTGCTG      360     - TGGACGTGGC CTTTTAACAC ACCCGTGGCT CTGCCCCGCT GCTCAGAGAT GG - #TGCCCGGC      420     - GCGGCCGACT GCAACATCAC TGCCGACTCC AGTGTGTACC CACAGGCAGA CG - #CGGTCATC      480     - GTGCACCACT GGGATATCAT GTACAACCCC AGTGCCAACC TCCCGCCCCC CA - #CCAGGCCG      540     - CAGGGGCAGC GCTGGATCTG GTTCAGCATG GAGTCCCCCA GCAACTGCCG GC - #ACCTGGAA      600     - GCCCTGGACG GATACTTCAA TCTCACCATG TCCTACCGCA GCGACTCCGA CA - #TCTTCACG      660     - CCCTACGGCT GGCTGGAGCC GTGGTCCGGC CAGCCTGCCC ACCCACCGCT CA - #ACCTCTCG      720     - GCCAAGACCG AGCTGGTGGC CTGGGCGGTG TCCAACTGGA AGCCGGACTC GG - #CCAGGGTG      780     - CGCTACTACC AGAGCCTGCA GGCTCATCTC AAGGTGGACG TGTACGGACG CT - #CCCACAAG      840     - CCCCTGCCCA AGGGGACCAT GATGGAGACG CTGTCCCGGT ACAAGTTCTA TC - #TGGCCTTC      900     - GAGAACTCCT TGCACCCCGA CTACATCACC GAGAAGCTGT GGAGGAACGC CC - #TGGAGGCC      960     - TGGGCCGTGC CCGTGGTGCT GGGCCCCAGC AGAAGCAACT ACGAGAGGTT CC - #TGCCGCCC     1020     - GACGCCTTCA TCCACGTGGA TGACTTCCAG AGCCCCAAGG ACCTGGCCCG GT - #ACCTGCAG     1080     - GAGCTGGACA AGGACCACGC CCGCTACCTG AGCTACTTTC GCTGGCGGGA GA - #CGCTGCGG     1140     - CCTCGCTCCT TCAGCTGGGC ACTGGCTTTC TGCAAGGCCT GCTGGAAGCT GC - #AGCAGGAA     1200     - TCCAGGTACC AGACGGTGCG CAGCATAGCG GCTTGGTTCA CCTGAGAGGC CG - #GCATGGGG     1260     - CCTGGGCTGC CAGGGACCTC ACTTTCCCAG GGCCTCACCT ACCTAGGGTC TC - #TAGA     1316     - (2) INFORMATION FOR SEQ ID NO:11:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 374 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:     - Met Asp Pro Leu Gly Pro Ala Lys Pro Gln Tr - #p Leu Trp Arg Arg Cys     #                15     - Leu Ala Gly Leu Leu Phe Gln Leu Leu Val Al - #a Val Cys Phe Phe Ser     #            30     - Tyr Leu Arg Val Ser Arg Asp Asp Ala Thr Gl - #y Ser Pro Arg Pro Gly     #        45     - Leu Met Ala Val Glu Pro Val Thr Gly Ala Pr - #o Asn Gly Ser Arg Cys     #    60     - Gln Asp Ser Met Ala Thr Pro Ala His Pro Th - #r Leu Leu Ile Leu Leu     #80     - Trp Thr Trp Pro Phe Asn Thr Pro Val Ala Le - #u Pro Arg Cys Ser Glu     #                95     - Met Val Pro Gly Ala Ala Asp Cys Asn Ile Th - #r Ala Asp Ser Ser Val     #           110     - Tyr Pro Gln Ala Asp Ala Val Ile Val His Hi - #s Trp Asp Ile Met Tyr     #       125     - Asn Pro Ser Ala Asn Leu Pro Pro Pro Thr Ar - #g Pro Gln Gly Gln Arg     #   140     - Trp Ile Trp Phe Ser Met Glu Ser Pro Ser As - #n Cys Arg His Leu Glu     145                 1 - #50                 1 - #55                 1 -     #60     - Ala Leu Asp Gly Tyr Phe Asn Leu Thr Met Se - #r Tyr Arg Ser Asp Ser     #               175     - Asp Ile Phe Thr Pro Tyr Gly Trp Leu Glu Pr - #o Trp Ser Gly Gln Pro     #           190     - Ala His Pro Pro Leu Asn Leu Ser Ala Lys Th - #r Glu Leu Val Ala Trp     #       205     - Ala Val Ser Asn Trp Lys Pro Asp Ser Ala Ar - #g Val Arg Tyr Tyr Gln     #   220     - Ser Leu Gln Ala His Leu Lys Val Asp Val Ty - #r Gly Arg Ser His Lys     225                 2 - #30                 2 - #35                 2 -     #40     - Pro Leu Pro Lys Gly Thr Met Met Glu Thr Le - #u Ser Arg Tyr Lys Phe     #               255     - Tyr Leu Ala Phe Glu Asn Ser Leu His Pro As - #p Tyr Ile Thr Glu Lys     #           270     - Leu Trp Arg Asn Ala Leu Glu Ala Trp Ala Va - #l Pro Val Val Leu Gly     #       285     - Pro Ser Arg Ser Asn Tyr Glu Arg Phe Leu Pr - #o Pro Asp Ala Phe Ile     #   300     - His Val Asp Asp Phe Gln Ser Pro Lys Asp Le - #u Ala Arg Tyr Leu Gln     305                 3 - #10                 3 - #15                 3 -     #20     - Glu Leu Asp Lys Asp His Ala Arg Tyr Leu Se - #r Tyr Phe Arg Trp Arg     #               335     - Glu Thr Leu Arg Pro Arg Ser Phe Ser Trp Al - #a Leu Ala Phe Cys Lys     #           350     - Ala Cys Trp Lys Leu Gln Gln Glu Ser Arg Ty - #r Gln Thr Val Arg Ser     #       365     - Ile Ala Ala Trp Phe Thr         370     - (2) INFORMATION FOR SEQ ID NO:12:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 1086 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:     - ATGGATCCCC TGGGTGCAGC CAAGCCACAA TGGCCATGGC GCCGCTGTCT GG - #CCGCACTG       60     - CTATTTCAGC TGCTGGTGGC TGTGTGTTTC TTCTCCTACC TGCGTGTGTC CC - #GAGACGAT      120     - GCCACTGGAT CCCCTAGGGC TCCCAGTGGG TCCTCCCGAC AGGACACCAC TC - #CCACCCGC      180     - CCCACCCTCC TGATCCTGCT ATGGACATGG CCTTTCCACA TCCCTGTGGC TC - #TGTCCCGC      240     - TGTTCAGAGA TGGTGCCCGG CACAGCCGAC TGCCACATCA CTGCCGACCG CA - #AGGTGTAC      300     - CCACAGGCAG ACACGGTCAT CGTGCACCAC TGGGATATCA TGTCCAACCC TA - #AGTCACGC      360     - CTCCCACCTT CCCCGAGGCC GCAGGGGCAG CGCTGGATCT GGTTCAACTT GG - #AGCCACCC      420     - CCTAACTGCC AGCACCTGGA AGCCCTGGAC AGATACTTCA ATCTCACCAT GT - #CCTACCGC      480     - AGCGACTCCG ACATCTTCAC GCCCTACGGC TGGCTGGAGC CGTGGTCCGG CC - #AGCCTGCC      540     - CACCCACCGC TCAACCTCTC GGCCAAGACC GAGCTGGTGG CCTGGGCGGT GT - #CCAACTGG      600     - AAGCCGGACT CAGCCAGGGT GCGCTACTAC CAGAGCCTGC AGGCTCATCT CA - #AGGTGGAC      660     - GTGTACGGAC GCTCCCACAA GCCCCTGCCC AAGGGGACCA TGATGGAGAC GC - #TGTCCCGG      720     - TACAAGTTCT ACCTGGCCTT CGAGAACTCC TTGCACCCCG ACTACATCAC CG - #AGAAGCTG      780     - TGGAGGAACG CCCTGGAGGC CTGGGCCGTG CCCGTGGTGC TGGGCCCCAG CA - #GAAGCAAC      840     - TACGAGAGGT TCCTGCCACC CGACGCCTTC ATCCACGTGG ACGACTTCCA GA - #GCCCCAAG      900     - GACCTGGCCC GGTACCTGCA GGAGCTGGAC AAGGACCACG CCCGCTACCT GA - #GCTACTTT      960     - CGCTGGCGGG AGACGCTGCG GCCTCGCTCC TTCAGCTGGG CACTGGATTT CT - #GCAAGGCC     1020     - TGCTGGAAAC TGCAGCAGGA ATCCAGGTAC CAGACGGTGC GCAGCATAGC GG - #CTTGGTTC     1080     #         1086     - (2) INFORMATION FOR SEQ ID NO:13:     -      (i) SEQUENCE CHARACTERISTICS:     #pairs    (A) LENGTH: 1654 base               (B) TYPE: nucleic acid               (C) STRANDEDNESS: unknown               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: DNA (genomic)     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:     - TTTTCTCATC TGTGAAACAG GAATAATAAC AGCTCTTCTC AGGACTCATG GC - #CTGGAGCT       60     - TTGGTAAGCA GGAGATTGTC ATCAATGACC CTCACTCCTC TCTCCCCACT TC - #CCAGAGAC      120     - TCTGACCCAT GGATCCCCTG GGCCCGGCCA AGCCACAGTG GTCGTGGCGC TG - #CTGTCTGA      180     - CCACGCTGCT GTTTCAGCTG CTGATGGCTG TGTGTTTCTT CTCCTATCTG CG - #TGTGTCTC      240     - AAGACGATCC CACTGTGTAC CCTAATGGGT CCCGCTTCCC AGACAGCACA GG - #GACCCCCG      300     - CCCACTCCAT CCCCCTGATC CTGCTGTGGA CGTGGCCTTT TAACAAACCC AT - #AGCTCTGC      360     - CCCGCTGCTC AGAGATGGTG CCTGGCACGG CTGACTGCAA CATCACTGCC GA - #CCGCAAGG      420     - TGTATCCACA GGCAGACGCG GTCATCGTGC ACCACCGAGA GGTCATGTAC AA - #CCCCAGTG      480     - CCCAGCTCCC ACGCTCCCCG AGGCGGCAGG GGCAGCGATG GATCTGGTTC AG - #CATGGAGT      540     - CCCCAAGCCA CTGCTGGCAG CTGAAAGCCA TGGACGGATA CTTCAATCTC AC - #CATGTCCT      600     - ACCGCAGCGA CTCCGACATC TTCACGCCCT ACGGCTGGCT GGAGCCGTGG TC - #CGGCCAGC      660     - CTGCCCACCC ACCGCTCAAC CTCTCGGCCA AGACCGAGCT GGTGGCCTGG GC - #AGTGTCCA      720     - ACTGGGGGCC AAACTCCGCC AGGGTGCGCT ACTACCAGAG CCTGCAGGCC CA - #TCTCAAGG      780     - TGGACGTGTA CGGACGCTCC CACAAGCCCC TGCCCCAGGG AACCATGATG GA - #GACGCTGT      840     - CCCGGTACAA GTTCTATCTG GCCTTCGAGA ACTCCTTGCA CCCCGACTAC AT - #CACCGAGA      900     - AGCTGTGGAG GAACGCCCTG GAGGCCTGGG CCGTGCCCGT GGTGCTGGGC CC - #CAGCAGAA      960     - GCAACTACGA GAGGTTCCTG CCACCCGACG CCTTCATCCA CGTGGACGAC TT - #CCAGAGCC     1020     - CCAAGGACCT GGCCCGGTAC CTGCAGGAGC TGGACAAGGA CCACGCCCGC TA - #CCTGAGCT     1080     - ACTTTCGCTG GCGGGAGACG CTGCGGCCTC GCTCCTTCAG CTGGGCACTC GC - #TTTCTGCA     1140     - AGGCCTGCTG GAAACTGCAG GAGGAATCCA GGTACCAGAC ACGCGGCATA GC - #GGCTTGGT     1200     - TCACCTGAGA GGCTGGTGTG GGGCCTGGGC TGCCAGGAAC CTCATTTTCC TG - #GGGCCTCA     1260     - CCTGAGTGGG GGCCTCATCT ACCTAAGGAC TCGTTTGCCT GAAGCTTCAC CT - #GCCTGAGG     1320     - ACTCACCTGC CTGGGACGGT CACCTGTTGC AGCTTCACCT GCCTGGGGAT TC - #ACCTACCT     1380     - GGGTCCTCAC TTTCCTGGGG CCTCACCTGC TGGAGTCTTC GGTGGCCAGG TA - #TGTCCCTT     1440     - ACCTGGGATT TCACATGCTG GCTTCCAGGA GCGTCCCCTG CGGAAGCCTG GC - #CTGCTGGG     1500     - GATGTCTCCT GGGGACTTTG CCTACTGGGG ACCTCGGCTG TTGGGGACTT TA - #CCTGCTGG     1560     - GACCTGCTCC CAGAGACCTT CCACACTGAA TCTCACCTGC TAGGAGCCTC AC - #CTGCTGGG     1620     #      1654        CACT GGGCCCTGGG AACT     - (2) INFORMATION FOR SEQ ID NO:14:     -      (i) SEQUENCE CHARACTERISTICS:     #acids    (A) LENGTH: 359 amino               (B) TYPE: amino acid               (D) TOPOLOGY: unknown     -     (ii) MOLECULE TYPE: protein     -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:     - Met Asp Pro Leu Gly Pro Ala Lys Pro Gln Tr - #p Ser Trp Arg Cys Cys     #                15     - Leu Thr Thr Leu Leu Phe Gln Leu Leu Met Al - #a Val Cys Phe Phe Ser     #            30     - Tyr Leu Arg Val Ser Gln Asp Asp Pro Thr Va - #l Tyr Pro Asn Gly Ser     #        45     - Arg Phe Pro Asp Ser Thr Gly Thr Pro Ala Hi - #s Ser Ile Pro Leu Ile     #    60     - Leu Leu Trp Thr Trp Pro Phe Asn Lys Pro Il - #e Ala Leu Pro Arg Cys     #80     - Ser Glu Met Val Pro Gly Thr Ala Asp Cys As - #n Ile Thr Ala Asp Arg     #                95     - Lys Val Tyr Pro Gln Ala Asp Ala Val Ile Va - #l His His Arg Glu Val     #           110     - Met Tyr Asn Pro Ser Ala Gln Leu Pro Arg Se - #r Pro Arg Arg Gln Gly     #       125     - Gln Arg Trp Ile Trp Phe Ser Met Glu Ser Pr - #o Ser His Cys Trp Gln     #   140     - Leu Lys Ala Met Asp Gly Tyr Phe Asn Leu Th - #r Met Ser Tyr Arg Ser     145                 1 - #50                 1 - #55                 1 -     #60     - Asp Ser Asp Ile Phe Thr Pro Tyr Gly Trp Le - #u Glu Pro Trp Ser Gly     #               175     - Gln Pro Ala His Pro Pro Leu Asn Leu Ser Al - #a Lys Thr Glu Leu Val     #           190     - Ala Trp Ala Val Ser Asn Trp Gly Pro Asn Se - #r Ala Arg Val Arg Tyr     #       205     - Tyr Gln Ser Leu Gln Ala His Leu Lys Val As - #p Val Tyr Gly Arg Ser     #   220     - His Lys Pro Leu Pro Gln Gly Thr Met Met Gl - #u Thr Leu Ser Arg Tyr     225                 2 - #30                 2 - #35                 2 -     #40     - Lys Phe Tyr Leu Ala Phe Glu Asn Ser Leu Hi - #s Pro Asp Tyr Ile Thr     #               255     - Glu Lys Leu Trp Arg Asn Ala Leu Glu Ala Tr - #p Ala Val Pro Val Val     #           270     - Leu Gly Pro Ser Arg Ser Asn Tyr Glu Arg Ph - #e Leu Pro Pro Asp Ala     #       285     - Phe Ile His Val Asp Asp Phe Gln Ser Pro Ly - #s Asp Leu Ala Arg Tyr     #   300     - Leu Gln Glu Leu Asp Lys Asp His Ala Arg Ty - #r Leu Ser Tyr Phe Arg     305                 3 - #10                 3 - #15                 3 -     #20     - Trp Arg Glu Thr Leu Arg Pro Arg Ser Phe Se - #r Trp Ala Leu Ala Phe     #               335     - Cys Lys Ala Cys Trp Lys Leu Gln Glu Glu Se - #r Arg Tyr Gln Thr Arg     #           350     - Gly Ile Ala Ala Trp Phe Thr             355     __________________________________________________________________________ 

What is claimed as new and desired to be secured by Letters Patent of the United States is:
 1. An isolated DNA fragment, comprising a nucleotide sequence which encodes the amino acid sequence of SEQ ID NO:11.
 2. The DNA fragment of claim 1, wherein said nucleotide sequence has the sequence of SEQ ID NO:10.
 3. The DNA fragment of claim 1, wherein said nucleotide sequence has the sequence of from position 247 to position 1111 of SEQ ID NO:10.
 4. A vector, comprising a DNA sequence which encodes the amino acid sequence of SEQ ID NO:11.
 5. The vector of claim 4, wherein said DNA sequence comprises from base 247 to base 1111 of SEQ ID NO:10.
 6. The vector of claim 4, wherein said DNA sequence comprises SEQ ID NO:10.
 7. A transformed microorganism, comprising a vector, said vector comprising a DNA sequence which encodes the amino acid sequence of SEQ ID NO:11.
 8. The transformed microorganism of claim 7, wherein said DNA sequence comprises from base 247 to base 1111 of SEQ ID NO:10.
 9. The transformed microorganism of claim 7, wherein said DNA sequence comprises SEQ ID NO:10.
 10. An isolated DNA fragment, comprising a nucleotide sequence which encodes the amino acid sequence of from position 43 to position 374 of SEQ ID NO:11.
 11. The DNA fragment of claim 10, wherein said nucleotide sequence has the sequence of SEQ ID NO:10.
 12. The DNA fragment of claim 10, wherein said nucleotide sequence has the sequence of from position 247 to position 1111 of SEQ ID NO:10.
 13. A vector, comprising a DNA sequence which encodes the amino acid sequence of from position 43 to position 374 of SEQ ID NO:11.
 14. The vector of claim 13, wherein said DNA sequence comprises from base 247 to base 1111 of SEQ ID NO:10.
 15. The vector of claim 13, wherein said DNA sequence comprises SEQ ID NO:10.
 16. A transformed microorganism, comprising a vector, said vector comprising a DNA sequence which encodes the amino acid sequence of from position 43 to position 374 SEQ ID NO:11.
 17. The transformed microorganism of claim 16, wherein said DNA sequence comprises from base 247 to base 1111 of SEQ ID NO:10.
 18. The transformed microorganism of claim 16, wherein said DNA sequence comprises SEQ ID NO:10. 